Effect of mfn2 on mitochondrial function in steatosis hepatocytes

AIM: To investigate the effect of mfn2 on mitochondrial function in steatosis hepatocytes. METHODS: Plasmid pEGFP-mfn2 was transfected into hepatocyte strain L02 by Lipofectamine 2000 in vitro, then the steatosis model of hepatocytes was establish by oleic acid induction. RT-PCR was used to evaluate mRNA expression and Western blotting was use to detect the protein expression. ATP level was determined by firefly luciferase bioluminescent. ROS production was measured by fluorescence probe DCFH-DA. Chondrosome transmembrane potential of L02 was observed by labeling of JC-1 and FCM. RESULTS: The stable expression of ectogenesis mitofusin2 in L02 cells was confirmed by RT-PCR and Western blotting. In the model of oleic acids induced lipid formation, Mfn2 obviously inhibited the descent of chondrosome transmembrane potential and ATP level, and increased ROS production in L02 cells. CONCLUSION: Up-regulated expression of mfn2 attenuates mitochondria dysfunction caused by oleic acids induced lipid formation.     

进口棉花数字化样品标识系统的建立

针对进口棉花到货时间相对集中、检测工作量大、检验人员和仪器设备配置相对不足的现状,开发了进口棉花数字化样品标识系统。本文介绍了该系统的开发背景、硬件构成、系统架构和数据库管理设计,分析了其取代纸质抄录样品标识的优势,如通过实现样品标识信息传递自动化、检验数据传递及其处理的自动化提升工作效率。     

高密度二氧化碳对蛋清液中沙门氏菌和大肠杆菌杀菌效果和杀菌动力学的研究

研究了不同温度、压力和时间条件下,高密度二氧化碳(DPCD)处理对蛋清液中沙门氏菌和大肠杆菌的杀菌效果,并对DPCD处理蛋清液中大肠杆菌的杀菌动力学进行了分析。结果表明,在15 MPa,45℃下DPCD处理60 min,沙门氏菌和大肠杆菌分别降低了4.46和5.57个对数值,其中大肠杆菌对DPCD处理较沙门氏菌敏感。30 MPa,45℃,DPCD处理30 min,可以完全杀灭蛋清液中的大肠杆菌。线性模型能较好地拟合DPCD对大肠杆菌的杀菌效果,其中30 MPa,45℃下D值最小为5.830。     

The influence of wax-sizing on dimension stability and mechanical properties of bagasse particleboard

Bagasse particleboards (BPBs) were made using polymeric methylene diphenyl diisocyanate (pMDI) resin as binder and wax emulsion as dimension stabilizer. A factorial experiment was conducted to measure the effects of wax and pMDI resin content on particleboard dimension stability and mechanical properties. The data were compared with respective properties specified in the ANSI A208.1 standard for commercial M3 grade wood-based particleboard.Wax-sizing improved the linear expansion (LE) of the particleboards under both pMDI resin contents used in this research and all LE values were controlled under the critical value of 0.35%. The use of wax significantly reduced 24-h water absorption and thickness swelling compared to the control panels without wax. Wax-sizing at the moderate levels also showed positive influence on long-term water absorption and thickness swelling properties. Wax content levels, however, did not significantly influence water absorption and thickness swelling behavior. Wax-sizing had no evident negative effects on the bending properties of MDI-bonded bagasse particleboards under both resin contents, while it caused slightly negative effect on internal bond strength. Mechanical properties of all boards far exceeded the minimum values specified in ANSI A208.1 standard. The entire properties of the 5% pMDI BPBs were better than those of the 3% pMDI panels as expected.     

三个典型植烟生态区土壤养分适宜性评价

通过明确不同烤烟种植区土壤肥力状况及植烟适宜性,为不同生态区烟草科学施肥方案的制定奠定基础。采用描述统计法、相关系数法和模糊综合评价法对四川会东、河南洛阳和安徽皖南3个植烟生态区的418份土壤样品进行分析。结果表明,会东烟区土壤pH为5.36～8.66,77.88%的土壤pH大于7.50,有机质含量较低,67%的土壤碱解氮含量低于90mg/kg,全氮含量适中,速效磷和速效钾含量丰富;洛阳烟区土壤pH呈弱碱性,有机质和碱解氮含量匮乏,70%以上的土壤有机质和碱解氮处于“缺”及以下水平,全氮和速效磷含量适中,速效磷含量变异系数达79.38%,速效钾含量丰富;皖南烟区土壤pH、有机质和全氮含量适中,碱解氮含量较高,速效钾含量缺乏,82.82%的土壤速效钾含量低于150mg/kg,速效磷含量丰富但异质性强。3个生态区植烟土壤综合肥力指数（IFI）存在显著差异（P<0.05）,表现为皖南（0.69）>会东（0.52）>洛阳（0.43）,其中皖南烟区75.76%的土壤IFI处于Ⅱ级以上,会东烟区处于Ⅱ级和Ⅲ级的比例分别为31.73%和46.15%,而洛阳烟区近50%的土壤为Ⅳ级。...     

稻瘟病菌无毒基因研究进展

水稻与稻瘟菌间存在广泛而特异的相互作用,是研究寄主与病原物互作的重要模式系统。无毒基因是病原物遗传因子,能诱发寄主植物产生抗病性,是稻瘟菌与水稻互作最重要的激发子。为寻求稻瘟病防治的新策略,本研究概述了研究稻瘟病菌无毒基因的意义,归纳了抗性基因与无毒基因互作的3种模式,总结了国内外稻瘟病菌无毒基因的克隆及功能研究,探讨了无毒基因在生产上病害流行预测中的应用,指出利用抗性基因与无毒基因的关系对稻瘟菌分类将会更为科学和实用,应进一步加快稻瘟病菌无毒基因的克隆。     

胚龄和基因型对小麦幼胚体细胞胚性无性系的诱导

幼胚胚龄和供体植株的基因型都会影响小麦幼胚体细胞胚性无性系的产生。胚龄对小麦幼胚体细胞胚性无性系的高频率诱导有显著影响 ,表现为愈伤组织诱导率 (出愈率 )和胚性愈伤组织诱导率在不同胚龄下差异显著。在最适胚龄条件下 ,基因型对小麦幼胚培养出愈率的影响不大 ,而主要影响胚性愈伤组织的发生频率。在培养当代 ,以小偃 2 2、千斤早和西农 88(1)的培养效果为最好     

简析我国农用三轮车产业现状

对我国农用三轮车产业发展阶段及产业特征进行分析,用数据说明了该产业的市场分布状况;同时对农用三轮车消费人群的消费心理、消费习惯进行深入分析,最后指出国家政策法规对农用三轮车产业发展的制约和影响。     

福建省地级市代谢系统差异性研究

以福建省9个地级市的代谢系统为研究对象,根据城市代谢的基本原理,运用能值理论和城市代谢系统互动关系分析方法,从代谢系统生态流和代谢系统中自然子系统与社会经济子系统之间的互动关系这两个方面对9个地级市代谢系统差异性研究,并结合GIS技术对其结果进行等级划分和空间差异性比较。结果表明代谢系统的可更新资源能值投入和进出口能值存在明显的海陆差异性;代谢系统的不可更新资源能值投入存在明显的南北差异性;福州市、厦门市、泉州市和漳州市这4个地级市代谢系统中自然生态子系统对社会经济子系统存在着限制关系,三明市、南平市、龙岩市、宁德市和莆田市这5个地级市代谢系统的社会经济子系统对自然生态子系统存在掠夺关系。     

Responses of soil enzyme activity and microbial community compositions to nitrogen addition in bulk and microaggregate soil in the temperate steppe of Inner Mongolia

In order to explore the responses of soil enzyme activities and microbial community compositions to long-term nitrogen (N) addition in both bulk soil and microaggregate of chestnut soil, we conducted a 7-year urea addition experiment with N treatments at 6 levels (0, 56, 112, 224, 392 and 560 kg N ha^–1 yr^–1) in a temperate steppe of Inner Mongolia in China. Soil properties and the activities of four enzymes involved in carbon (C), nitrogen (N) and phosphorus (P) cycling were measured in both bulk soil and microaggregate, and phospholipid fatty acids (PLFAs) were measured in bulk soil. The results indicated that: 1) in bulk soil, N addition significantly decreased β-1,4-glucosidase (BG) and leucine aminopeptidase (LAP) activities at the treatment amounts of 224, 392 and 560 kg N ha^–1 yr^–1, and obviously suppressed β-1,4-N-acetylglucosaminidase (NAG) activity at the treatment amount of 560 kg N ha^–1 yr^–1. N addition enhanced total PLFAs (totPLFAs) and bacterial PLFAs (bacPLFAs) at the treatment amounts of 392 and 560 kg N ha^–1 yr^–1, respectively, but fungal PLFAs showed no response to N addition. The activities of BG, NAG and LAP were positively correlated with soil pH, but negatively correlated with the concentration of NH ₄⁺-N; 2) in microaggregate (53–250 μm), the activities of BG, NAG and AP showed no response to increased addition of N, but the significantly decreased LAP activity was observed at the treatment amount of 392 kg N ha^–1 yr^–1. These results suggested that enzyme activities were more sensitive to N addition than PLFA biomarkers in soil, and LAP activity in microaggregate may be a good indicator for evaluating N cycle response to long-term N addition.