秋延大棚网纹甜瓜改良型有机生态无土基质栽培技术

网纹甜瓜原产于非洲、中亚大陆，属于葫芦科甜瓜属厚皮甜瓜。其外形美观，营养丰富，质脆味甜气香，惹人喜爱，是饭店、宾馆、馈赠礼品及家庭鲜食的热销水果。近年来我市在基质无土栽培的基础上，改良了基质配方，成功地进行了网纹甜瓜的秋延栽培，探索出了一套亩产在2500kg以上的改良型有机生态无土栽培的相关配套技术，现将其总结如下：     

新《兽药管理条例》实施后化药技术审评工作介绍

新《兽药管理条例》实施后的化药技术审评工作也是企业关心的一个热点,此篇为汪霞在2004年12月25～26日,中国动物保健品协会举办的“兽药地方标准清理研讨班”上的讲稿,本刊特刊出,以期能对兽药企业的工作有所指导。     

Expression of endostatin in ischemic myocardium of myocardial infarction rats

AIM: To study the expression of endostatin in ischemic myocardium of myocardial infarction (MI) rats in various periods and the correlation with VEGF expression and microvascular density (MVD).METHODS: Thirty-two male Sprague-Dawley rats after myocardial infarction were randomly divided into 7, 14, 21 and 28 days group.The sham group was normal control group (eight rats in each group).The expression of endostatin, VEGF and MVD in ischemic myocardium were observed by immunohistochemistry.RESULTS: The expression of endostatin significantly increased in the ischemic myocardium after MI, peaked at 7 days, then gradually decreased at 14, 21 and 28 days.The endostatin level at 28 days was the same as the shams.The changing trends of expression of endostatin in ischemic myocardium after MI were similar to that of VEGF and were significantly correlated with the MVD.CONCLUSION: The expression of endostatin increased in ischemic myocardium of myocardial infarction rats.The changing trends of endostatin were similar to that of VEGF and positively correlated with the MVD.These data suggest that endostatin may modulate ischemic myocardium angiogenesis after myocardial infarction.     

浅析皖南建立竹造纸厂的可行性

随着木材资源的紧缺,以竹浆为原料的造纸产业应运而生。安徽皖南山区毛竹的种植面积广,且形成一定规模。因此可就地建厂,进行竹浆造纸,实现以竹代木做浆,建立林纸一体化的竹浆生产基地。这可成为节约木材资源,增加农民收入,振兴皖南经济,并使制浆造纸工业得以持续发展的有效途径。本文对以竹造纸及在皖南地区建厂的可行性作一分析。     

细胞培养的脑炎原虫感染家兔实验

用在体外连续培养１０代的兔脑炎原虫，接种于１２只家兔，动态地观察了脑炎原虫对家免的感染情况。实验发现，肾脏产生的病变明显早于大脑，即感染３周后，用Ｇｒａｍ＇ｓ和Ｇｏｏｄｐａｓｔｕｒｅ＇ｓ染色，即可在髓袢和远曲小管内脱落的上皮细胞中发现兔脑炎原虫。６周后即可见到间质性肾炎变化，而脑组织于感染９～１２周时才见有细胞性肉芽肿的形成；脑炎原虫具有较强的抗原性，即在感染３周后，血液中的抗体含量足以进行诊断。     

不同培养条件对赤霉病菌毒素产生的影响

 本试验以MS培养基为基本培养基,研究不同培养条件对赤霉病菌的产毒能力及其形态指标。     

基于主从策略的两层约束变尺度决策方法

本文针对上层为多目标，下层多人多目标且具有优先级的两层决策问题，建立了其数学模型和决策机制，随后提出了一种交互式决策方法，该方法具有交互过程简单，计算量较小等优点。     

胭脂萝卜新品种胭脂红3号的选育

胭脂红3 号是以不育系09S77A 为母本，以自交系0484B 为父本配制而成的胭脂萝卜一代杂种。播种至收获100～120 d（天），羽状裂叶，叶片绿色，叶柄及叶脉紫红色；肉质根短圆锥形，皮紫红色，肉深红色，横径7 cm 左右，纵径8 cm 左右，单根质量250 g 左右，色素含量17‰左右；每667 m² 产量1 800 kg 左右，适宜重庆地区作色素提取和泡菜加工品种栽培。     

长白猪血浆酶活性与胴体性状的相关研究

采用通用线性模型分析研究了 4种猪血浆酶活性与 4个胴体性状的相关关系。结果表明 :GPT对皮厚、γ- GT对胴体长达到显著的正效应 (P<0 .0 5 ) ,Amy对眼肌面积达到极显著正效应 (P<0 .0 1)     

Cloning and expression of interferon-alpha/gamma from a domestic porcine breed and its effect on classical swine fever virus

To further evaluate the clinical impact of recombinant PoIFN-alpha/gamma, PoIFN-alpha/gamma genes from a Chinese domestic big-white porcine breed were cloned using PCR, and expressed in a high-level prokaryotic system. The antiviral activities of rPoIFN-alpha/gamma on vesicular stomatitis virus (VSV), porcine reproductive and respiratory syndrome virus (PRRSV), and classical swine fever virus (CSFV) were investigated in different cell lines. The cloned PoIFN-alpha gene encodes a protein of 166 amino acids and has been named PoIFN-alphac. In a comparison of PoIFN-alphac with reported PoIFN-alphaI genes, eight amino acid substitutions at positions 43 (F to L), 78 (N to D), 86 (Y to C), 104 (A to V), 118 (R to L), 128 (T to P), 151 (S to V), and 156 (R to T) were observed, and resulted in no potential N-glycosylation site in the deduced PoIFN-alpha amino acid sequences. In contrast to PoIFN-alphac, one nucleotide substitution was found at position 462 (A to G), hence 0.1% synonymity is specific for the PoIFN-gamma gene. Both PoIFN-alphac and PoIFN-gamma genes were inserted into a prokaryotic vector pQE30, and expressed in E. coli M15 (pREP4) or SC11103 (pREP4) with the N-terminal six consecutive histidine residues, respectively. rPoIFN-alphac and rPoIFN-gamma proteins were detected by SDS-PAGE and Western blotting analysis at 20.7 and 18.0 kDa, respectively. In addition, the rPoIFN-alphac and rPoIFN-gamma protein were purified using Ni-NTA metal-affinity chromatography, and their anti-VSV, anti-PRRSV, and anti-CSFV activities were surveyed in homologous and heterologous cell lines. The results suggested that rPoIFN-alpha and rPoIFN-gamma could inhibit classical swine fever virus and other important viral pathogens in different cell lines.