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111.
112.
宁夏中宁等县市区个体养猪户的猪出现以败血性出血和两耳、胸前、腹下、四肢皮肤呈现紫绀为特征的疾病,并引起猪群,尤其是仔猪大批死亡.经临床观察、病理剖检、实验实检查,确诊为猪附红细胞体病.经用金霉素、土霉素拌料饲喂,肌注血虫净及后期用强力霉素拌料喂服和肌注磷酸伯氨喹、青蒿素,病情获控制. 相似文献
113.
谢德琼 《四川畜牧兽医学院学报》2005,3(4):133-136
讨论了目前大学英语课程设置单一的现状,并分析其形成原因。文章以ESP研究关于需求分析的理论为依据,提出大学应根据学生的实际需要、新时期国家和社会对人才培养需要,区别不同层次、不同程度、不同专业学生的需求,而专门设置一系列大学英语课程。说明了设置个性化的大学英语课程的理由和好处。 相似文献
114.
本文从吉林省荒地资源的实际出发 ,在荒地资源生态适宜性评价基本理论的指导下 ,采用地理信息系统方法 ,对吉林省荒地资源进行了生态适宜性评价。通过空间数据和属性数据的融合、分析、处理 ,可以直观获得荒地资源的生态适宜度等级 ,从而提高评价效率 ,为荒地资源的合理开发利用提供科学依据。 相似文献
115.
AIM:To investigate the effect of cyclophosphamide(CTX) on proliferation and apoptosis of mesangial cells(GMC) of rat in vitro. METHODS:GMC proliferat ion was detected by MTT method,GMC apoptosis was examined by inverted microscopy for phase-contract and fluoroscopy and flow cytometry analysis.The levels of Fas and Bcl-2 were also detected by immunohistology. RESULTS:The proliferation of GMC were inhibited by CTX, methylprednisolone(MP), low molecular weight heparin(LMWH). Apoptosis of GMC was induced by CTX, the apoptosis rate of GMC was 8.2%, and the Fas level was increased. CONCLUSION:CTX could inhibit proliferation and induce apoptosis of GMC possibly by enhancing the Fas level. 相似文献
116.
117.
‘三棱榄''橄榄果实香气成分分析 总被引:7,自引:1,他引:7
1 材料与方法选取广东优良鲜食橄榄品种‘三棱榄’,2001年12月6日采样,采用固相微萃取法(SPME)富集香气成分(鲜橄榄果肉于15℃下捣碎后取样1.0 g放入4 mL聚四氟乙烯硅橡胶垫密封螺口玻璃瓶中,插入100μm聚二甲基硅氧烷纤维头于室温25-30℃顶空取样2 h),用美国Finnigan TRACE GC-MS气相色谱-质谱联用仪进行分析。气相色谱柱为DB-1弹性毛细管柱30 m×0.25 mm,载气为He(99.99%),流速1.0mL/min。程序升温从40℃开始先保持10 min,后以2℃/min的升温速率升至150℃保持10 min。质谱条件:电子能量70 eV,离子源温度250℃,质量范围35-450 aum,不分流进样。2002年12月18日采样重复分析。 相似文献
118.
LEI Jun-xia LI Hao-wei HUANG Chun-nong ZHU Mei-ling WEN Guan-mei ZHANG Xiu-ming LI Yan LI Shu-nong 《园艺学报》2003,19(10):1325-1330
AIM:To investigate multi-potential of rat bone marrow mesenchymal stem cells (rBMMSC) and mutation inclination, the rBMMSC were long passaged in vitro. METHODS:Cellular cycles of different passages were assayed by FACSan flow cytometry and karyotypes of passage 6, passage 25 and passage 45 were compared by G-binding analysis. RESULTS:The early passages and long-term passages all showed strong proliferation; passage 6, passage 25 and passage 45 all showed normal karyotype. CONCLUSION:Long-term culture and passage of rBMMSC still remains strong proliferation. With this capability, the mutation inclination is not enhanced. 相似文献
119.
ZHOU Yan-bin YE Ren-gao XIE Can-mao XU Han-shi GUAN Wei-ming YANG Xiao YANG Nian-sheng 《园艺学报》2003,19(9):1221-1223
AIM:To investigate the role of CD134 (OX40) and NF-κB in the pathogenesis of lupus nephritis (LN).METHODS:Renal in situ CD134 and NF-κB expression were examined in 40 biopsy specimens from LN patients by immunohistochemistry and microwave-based immunohistochemistry, respectively. The relationship between expression of CD134 and NF-κB was analyzed.RESULTS:The expression of glomerular and tubular CD134 and NF-κB in LN were higher than that in normal control, especially in class Ⅳ LN, where there was intense staining of endothelial cell, distal tubules, and interstitial mononuclear cell. The CD134 expression of glomerular and tubular was closely related to NF-κB expression, respectively (r=0.5542, P<0.05;r=0.6279, P<0.05). CONCLUSIONS:The abnormal expression of costimulatory molecule CD134 was well evidenced in LN. Strong expression of renal in situ NF-κB was likely mediated by CD134 signal pathway, which may play an important role in the pathogenesis of LN. 相似文献
120.
AIM:To investigate the effects of fibronectin (FN) on the proliferation and collagen synthesis in cultured rat cardiac fibroblasts (CFb) derived from SHR (CFbSHR) and WKY (CFbWKY). METHODS:CFb derived from 12-week-old spontaneously hypertensive rat (SHR) and WKY was cultured by outgrowth of tissue block. Cell proliferation of CFb was measured by cell number counting and[3H]-TdR incorporation using 24-well plates pre-coated with 5 μg/cm2 of FN. Collagen synthesis was determined by [3H]-proline incorporation. RESULTS:As compared with control, the cell number of fibroblasts derived from SHR and WKY were significantly increased to 163.75% and 170.42% respectively after 72 h incubation with FN in the presence of 0.4% FCS from a intial cell density of 1×104 cells/mL. DNA synthesis of CFb was markedly promoted by FN. FN induced an increased in [3H]-proline incorporation in both CFbSHR and CFbWKY. CONCLUSION:FN is able to promote cell proliferation and collagen synthesis of CFb derived both from SHR and WKY. 相似文献