番鸭新病——花肝病的研究：Ⅰ．分离毒的致病必及感染途径试验

番鸭花肝的是近年流行的一种新的番鸭疫病，其特征性病变是死亡番鸭的肝、脾、小肠等部位出现灰白色的坏死点，本文自广东省主要疫区分离到几株病毒，这些病毒在番鸭胚上可继代繁殖，其胚液可使番鸭妇病。不同途径感染试验表明该病毒通过肌肉注射、爪垫部注射、口服，同居感染均可使番鸭发病和死亡，并具有典型病变，分离毒不能使半番鸭，本地鸭，鸡发病。     

美国开放式保护主义及我国银行业安全的法律规制

美国银行业安全的法律规制经历了近百年的演进,形成了以紧跟金融创新发展步伐的渐进性,维护国家金融主权的保护性,寻求体制内各种法律制度互补的平衡性为特征的开放式保护主义法律规制体系.借鉴美国经验,我国在金融开放,实现金融现代化的进程中,也应以公益性、安全性、程序性等诉求为依据,以法律法规的定向约束为手段,以国家利益至上为宗旨,以对国外竞争者设置制度化的市场障碍和金融市场参与者稳健经营为目的,对我国银行业安全法律规制进行完善,以维护国家金融安全.     

ELISA鉴别条件下的野生羊肚菌菌种分离方法

为了提高分离的羊肚菌菌种的可靠性,在传统菌种分离的基础上,增加了酶联免疫吸附试验(ELISA)鉴别检验。对采自于吉林省吉林市的野生羊肚菌发生地的羊肚菌土壤基质及子实体分别进行了菌种分离,对分离得到的3株疑似羊肚菌的菌株进行了间接酶联免疫吸附法(间接ELISA)免疫学方法检测,确定其中的2株纯培养菌丝为羊肚菌菌种。     

Role of 17-AAG in inducing apoptosis and cell cycle arrest of HCT-15 cells

AIM: To investigate the effects of 17-AAG on apoptosis and cell cycle of HCT-15 cells and to clarify the related mechanisms. METHODS: MTT method was employed to evaluate the inhibitory effects of 17-AAG with Aifferent time and different doses on the proliferation of HCT-15 cells. The cells were stained with Annexin V-FITC/propidiumiodide and measured by flow cytometry. The expression of STAT3, cyclin D1, Cyt C, caspase 9 and caspase 3 at mRNA and protein levels was determined by RT-PCR and Western blotting. RESULTS: Treatment with 17-AAG at concentration of 1.25~20 mg/L for 24 h and 48 h significantly inhibited the activity of HCT-15 cells at both time-and concentration-dependent manners. Treatment with 17-AAG at concentrations of 0.425, 0.85 and 1.7 mg/L for 48 h significantly induced apoptosis and cell cycle arrest of HCT-15 cells. The exposure of 17-AAG at concentrations of 0.425, 0.85 and 1.7 mg/L for 48 h to the HCT-15 cells significantly down-regulated the expression of STAT3 and cyclin D1 at mRNA and protein levels, but up-regulated Cyt C, caspase 9 and caspase 3 mRNA and protein in a concentration-dependent manner. CONCLUSION: 17-AAG inhibits the cell activity, induces apoptosis and G₁ arrest by down-regulating the expression of cyclin D1, and promoting the mitochondria apoptosis through STAT3 pathway.     

香石竹DcMAPK1和DcMAPK2的克隆及功能初#br# 步分析

 分离了香石竹（Dianthus caryophyllus）两个促分裂原活化蛋白质激酶（mitogen-ac-tivated protein kinase,MAPK）基因cDNA 全长,分别命名为DcMAPK1 和DcMAPK2,其中DcMAPK1 与拟南 芥AtMAPK6 高度同源。DcMAPK1 在香石竹花瓣衰老过程表达增加,乙烯处理显著促进其表达,瞬时抑 制DcMAPK1 后切花瓶插寿命显著延长。而DcMAPK2 在花瓣衰老过程中组成型表达,乙烯处理对其表达 无显著影响,瞬时抑制DcMAPK2 对切花瓶插寿命无显著影响。     

海南省西甜瓜细菌性果斑病的发生与防治

<正>近年来细菌性果斑病在海南西瓜、甜瓜生产上发生严重,其原因不仅与当地气候条件、栽培管理不当等有关,也与各地进行南繁的瓜类品种繁杂、种子消毒不彻底、病原远距离传播扩散有关。西瓜、甜瓜是海南省种植面积较为广泛、经济效益较高的农产品,2013年种植面积达1.97万hm2(29.55万亩)(农业部,2015)。细菌性果斑病是西瓜、甜瓜生产上的一种毁灭性病害,引起该病的病原菌为西瓜嗜酸菌(Acidovorax citrulli)(Schaad     

蒋卫杰博士:聚焦生产一线(十三) 增城区菜薹(菜心)多茬高产高效栽培技术

<正>增城区菜心种植面积约3 500 hm2(5.25万亩)。当地菜农通过早熟、中熟和晚熟3种类型品种搭配种植,采用不同播种方式,勤施、早施、薄施肥,以及制水、制肥等系列生产措施,一年可以种植约8茬,每茬每667m2效益达3 000~4 500元。广东省广州市增城区菜薹(菜心)种植历史悠久,菜心类型和品种资源丰富,常年种植面积约3 500 hm2(5.25万亩),可周年生产、周年供应,复种指数高,一年可以种植约8茬,每茬每667 m2产量为1 000~1 500 kg,效益3 000~4 500元。     

Cx43 is involved in electrical remodeling of atrial myocytes through regulating L-type calcium current

AIM: To investigate whether the association of connexin 43(Cx43) and L-type calcium channel involved in the pathogenesis of atrial fibrillation (AF). METHODS: The biochemical assays and whole-cell patch-clamp technique were used to study the expression of Cx43 in human atrial tissue. The co-localization of Cx43 and L -type calcium channel, and the regulation of L-type calcium current in atrial myocytes were investigated. RESULTS: The expression of Cx43 at mRNA and protein levels was decreased in human atrial tissues of AF patients. In cultured atrium-derived myocytes (HL-1 cells), knockdown of Cx43 significantly inhibited the mRNA expression of L-type calcium channel α1c subunit, as well as L-type calcium current. Co-localization of Cx43 with L-type calcium channel α1c subunit in mouse atrial myocytes was observed. CONCLUSION: The decrease in Cx43 is involved in the pathogenesis of AF, probably through reducing the L-type calcium current in atrial myoctyes by co-localization with L-type calcium channel, thus representing the potential pathogenesis in atrial fibrillation.     

豫西黄土丘陵区生态环境条件分析与生态果园复合模式研究

为了更好地在豫西黄土丘陵区建设生态果园,分析了该区气候、地貌、土地、植被及水土流失等生态环境条件,介绍了"草-牧-沼-窖-果"、"草-牧-果"和以旅游观光为主的3种生态果园复合模式。并对生态果园建设中果园的规划和布局、水土保持措施建设、保水耕作技术及生物防治技术进行了分析和探讨,以期为生态果园建设提供理论基础和生产指导。