Ocular localization of mycobacterial lesions in tank‐reared juvenile cobia,Rachycentron canadum

Severe clinical mycobacteriosis with consistent ocular lesion localization was diagnosed in a population of 800 juvenile tank‐reared Cobia (Rachycentron canadum) which experienced a sudden increase in mortality approximately 5 months after arriving into Trinidad and Tobago from Florida, USA. Moderate daily mortality (15–20 animals per day) persisted for just over 1 month. Moribund fish displayed circling behaviour and had an open‐mouth gape upon death. Fish consistently presented with bilateral exophthalmia, corneal cloudiness and hyphema. Non‐branching acid‐fast rods were detected in aqueous humour touch preparations. Histological analysis revealed severe bilateral intra‐ocular granulomatous responses in all specimens. Mycobacterium sp. was identified using a real‐time PCR assay detecting the RNA polymerase β‐subunit (rpoB) gene in different tissue samples. Specimens did not present with characteristic granulomatous responses usually seen in viscera. To the best of our knowledge, this represents only the third documentation of piscine mycobacterial infection presenting with only localized ocular lesions, and the second documented case of mycobacteriosis in cobia. It is, however, the first documentation of an ocular presentation of mycobacteriosis in a marine species and is the first documentation of such a presentation in cobia.     

Potential Use of Raman Spectroscopy for Determination of Amylose Content in Maize Starch

An analytical method using Raman spectroscopy was developed for the determination of amylose concentration in maize starches. FT-Raman spectra of four maize starches with amylose content varying from 3.3 to 66% were obtained. A Raman band at ≈1657 cm^-1 correlated linearly with amylose concentration in the four maize starches, and a calibration curve for Raman band intensity versus amylose content was developed. The linear correlation of the I₁₆₅₇/I₉₀₀ integrated areas with amylose content was r = 0.997. The Raman-based calibration curve allows fast and nondestructive determination of the amylose content in maize starches with minimal sample preparation.     

Quantitative Analysis of Benzyl Modification in Waxy Maize Starch by Fourier Transform (FT) Raman Spectroscopy

Waxy maize starch was chemically modified to varying benzylation levels and the degree of benzylation substitution was measured using a nuclear magnetic resonance (NMR) method. Fourier Transform (FT) Raman spectra of the chemically modified starches were acquired and aromatic C=C stretch Raman bands characteristic of the benzylation modification were used to derive a calibration curve for the Raman intensity of these marker bands versus the degree of benzylation substitution. The best‐fit linear regression to the plotted data gave a linear correlation coefficient of 0.997. The FT‐Raman technique provides a fast, nondestructive method for the measurement of the degree of benzylation substitution of modified waxy maize starches and should be applicable for use with benzylated starches from other botanical sources.     

Implications of climate change scenarios for soil erosion potential in the USA

Atmospheric general circulation models (GCMs) project that increasing atmospheric concentrations of CO2 and other greenhouse gases May, result in global changes in temperature and precipitation over the next 40-100 years. Equilibrium climate scenarios from four GCMs run under doubled CO2 conditions were examined for their effect on the climatic potential for sheet and rill erosion in the conterminous USA. Changes in the mean annual rainfall factor (R) in the Universal Soil Loss Equation (USLE) were calculated for each cropland, pastureland and rangeland sample point in the 1987 National Resources Inventory. Projected annual precipitation changes were assumed to be from differences in either storm frequency or storm intensity. With all other USLE factors held constant these changes in R translated to changes in the sheet and rill erosion national average of +2 to +16 per cent in croplands, -2 to +10 per cent in pasturelands and -5 to +22 per cent in rangelands under the eight scenarios. Land with erosion rates above the soil loss tolerance (T) level and land classified as highly erodible (eredibility index >8) also increased slightly. the results varied from model to model, region to region and depended on the assumption of frequency versus intensity changes. These results show the range of sensitivity of soil erosion potential by water under projected climate change scenarios. However, actual changes in soil erosion could be mitigated by alterations in cropping patterns and other management practices, or possibly by increased crop growth and residue production under higher atmospheric CO2 concentrations.