松突圆蚧空间分布型的初步研究

昆虫的空间分布型是昆虫种群属性之一,也是昆虫生态学的内容。探明昆虫的空间分布型,可以深入了解其生物学特性,通过种群分布的信息,揭示种群的空间结构及分布形式,为确定抽样技术、取样数量和分析提供依据,对生态学理论和生产上都有重要意义。为探索松突圆蚧Hemiberlesia pitysophila Takagi分布特点,我们于1987～1988年,对广东省惠     

Immunolocalization of steroidogenic enzymes in the corpus luteum and placenta of the Japanese Shiba goat

In this study, we performed immunohistochemistry of cholesterol side-chain cleavage cytochrome P450 (P450scc), 3beta-hydroxysteroid dehydrogenase (3betaHSD), cytochrome 17alpha-hydroxylase P450 (P450c17), and cytochrome P450 aromatase (P450arom) in the corpus luteum and placenta of Shiba goats. The aim was to clarify the steroidogenic capability of the corpus luteum and placenta of Shiba goats. Ovaries containing corpora lutea were obtained from four adult Shiba goats during the luteal phase (day10; n=2) and pregnancy (90 and 120 days of gestation). Placenta was obtained from one Shiba goat on day 120 of gestation. The sections of the ovaries and placentae were immunostained using the avidin-biotin-peroxidase complex method (ABC) with polyclonal antibodies generated against steroidogenic enzymes of mammalian origin. All luteal cells expressed P450scc, 3betaHSD, P450c17 and P450arom. The distribution of P450scc, 3betaHSD, P450c17 and P450arom were not different during the luteal phase and pregnancy. P450arom showed a weak positive staining in late pregnancy (120 days). In addition, immunoreactions for P450c17 and P450arom were observed in syncytiotrophoblast of the placenta of one Shiba goat. These results indicate that, in Shiba goats, corpus luteum is not only an important source of progesterone but also has the ability to synthesize androgen and estrogen during the luteal phase and pregnancy. Also the placenta has the ability to synthesize androgen and estrogen in late pregnancy.     

Male Pseudohermaphroditism in a raccoon dog (Nyctereutes procynoides)

A wild raccoon dog (Nyctereutes procynoides) which died due to a traffic accident on 18 October 2001, and was determined to be 4.5 years old, was examined. Visual appearance of the external genitalia in this animal showed to be female with a large penis-like clitoris protruding from the vulvar juncture. Visual examination of the internal genitalia revealed that the animal possessed both testes and uterus. Histological appearance of the removed gonads showed only Sertoli cells but no spermatogenesis. Using polymerase chain reaction with skin biopsy directed against the sex-determining region on the Y chromosome (SRY) gene, the genomic SRY gene was expressed as a single band and sequenced. Based on these findings, this raccoon dog was diagnosed as male pseudohermaphrodite.     

Immunolocalization of nerve growth factor (NGF) and its receptors (TrkA and p75LNGFR) in the reproductive organs of Shiba goats

The objective of this study was to determine the immunolocalization of NGF and its receptors (TrkA and p75LNGFR) in the reproductive tract of the Japanese Shiba goats. Five adult goats were used in this study and sections of ovaries, uteri and oviducts were immunostained by the avidin-biotin-peroxidase complex method (ABC). The results showed that NGF and its receptors (TrkA and p75LNGFR) were expressed in granulosa cells, theca cells, interstitial cells and lutein cells in ovaries. Immunoreactions for NGF, TrkA and p75LNGFR were also detectable in epithelial cells and muscle cells of the ampulla and isthmus of the oviduct, and in epithelial cells and uterine glands of the uterus. These results strongly suggest autocrine and paracrine regulation of reproductive function by NGF in the reproductive tract of female Shiba goats.     

氮肥施用对柑橘果园土壤有机碳矿化的影响

土壤有机碳矿化是土壤向大气释放CO2的最大净输出途径,其与植被的净初级生产力的差值是判断生态系统碳“源”或“汇”的关键。采用室内模拟试验,在10、20、30℃ 3个温度条件下,研究施肥施用对柑橘果园土壤有机碳矿化的影响。结果表明：3种温度处理下,各施氮处理土壤有机碳矿化速率都表现为培养前期快速下降,培养后期保持相对稳定的趋势。在整个培养过程中,3种温度条件下各施氮处理的土壤CO2累积排放量为1328.25~2219.42 mg/kg,100 mg/kg（N4）处理土壤有机碳矿化量最大,CK处理最低,100 mg/kg（N4）和80 mg/kg（N3）2个高氮处理显著高于低氮50 mg/kg（N2）、30 mg/kg（N1）处理。土壤有机碳矿化速率随温度升高而增长,不同的土壤施氮条件下土壤有机碳矿化的温度敏感性不同,N2处理土壤有机碳矿化的温度敏感性最低,N4处理最高。柑橘果园土壤有机碳矿化受高施氮量影响较大,低施氮影响不明显。随着施氮量的增加土壤有机碳矿化的温度敏感性增加,氮肥施用和温度的共同作用可能使柑橘林向大气中排放的CO2增加。     

武汉地区三角梅遮光催花试验研究

本试验以1～3 a生的三角梅盆花为试材,以遮光处理为研究手段进行武汉地区三角梅花期调控试验探索,结果表明:在20～30℃的温度条件下,用栽培地布对三角梅进行连续遮光处理20 d,能完成稳定的花芽分化,继续遮光25 d可开花,并在此期间将腐熟的有机肥上清液稀释10倍灌根代替浇水能有效促进三角梅开花。     

杀球灵抗鸡球虫的药效研究

采有笼饲试验和围栏试验,研究了一种新的抗球虫药——杀球灵抗鸡球虫的药效。笼饲试验表明:杀球灵抗柔嫩艾美耳球虫的指数为195.6,属于高效抗球虫药.围栏试验表明:杀球灵以1ppm剂量添加于饲料,可有效地控制鸡的3种主要球虫——柔嫩、堆型和巨型艾美耳球虫的混合感染,可阻止死亡、抑制盲肠病变产生和卵囊形成,并能维持正常的增重和饲料转化率。     

河北省中药材生产存在的问题及对策

阐述了河北省药用植物责源现状,分析了河北省中药材生产存在的主要问题和应对措施。     

硒镧复合作用下姬松茸氨基酸数量性状的因子分析

对硒镧复合作用下姬松茸氨基酸数量性状进行因子分析。结果表明,硒镧复合作用下姬松茸氨基酸的17个数量性状,可提取为2个公因子,其累积方差贡献率达93.66%,具有较好地反映这些性状所包含的信息。按照主因子所包含的性状及其所反映的营养学和药学含义,可把2个主因子命名为芳香族氨基酸、支链氨基酸因子和鲜味氨基酸因子。一个2因子的模型能够合理地解释氨基酸含量间的相关关系。其因子得分可用于对硒镧复合作用下氨基酸质量的综合评价,证实氨基酸含量与硒镧复合作用下姬松茸含量之间大部分存在着极显著相关性.这些研究为以后姬松茸的栽培及营养学和药学机理提供科学依据。