恩施山区现代职业烟农队伍培育及发展对策——基于咸丰县烟农发展现状的思考

为促进恩施烟叶产业的可持续发展,以咸丰县为例,系统分析了该县烟农群体的现状、存在的问题及烟农流失的主要原因,探索了现代职业烟农的标准,针对性地提出现代职业烟农队伍培育及发展对策。咸丰县烟农的整体文化素质较低、烟农流失较快、单户规模偏大。应从建立现代职业烟农标准入手,完善服务和培训保障体系,构建土地集中流转、烟农动态考核管理、风险保障等机制,培育一批“以烟为主、诚实守信、有文化、懂技术、会经营”的新型职业烟农队伍,以促进咸丰县烤烟产业可持续发展。     

Male heterogametic sex determination in Rana dybowskii based on sex-linked molecular markers

Identifying the mechanism for sex determination in amphibians is challenging. Very little is known about sex determination mechanisms of Rana dybowskii, a species of importance to evolutionary and conservation biology. We screened for sex-linked molecular markers in R. dybowskii in China using target region amplification polymorphism with 2 fixed primers against the sequences of Dmrt1. We found 2 male-linked molecular markers in R. dybowskii, which were 222 bp and 261 bp long. The detection rates of 222 bp marker in males form Xinglong, Huadian, and Dandong were 93.79%, 69.64%, and 13.64%, respectively, while the rate in females from Huadian was 27.50%. Besides, the detection rates of 261 bp marker in the above 3 regions were only observed in males at the rate of 93.79%, 87.50%, and 32.73%, respectively. The inheritance patterns of sex-linked molecular markers showed that the 2 sex-linked molecular markers were heterozygous. Compared to the XY-male parent, progeny from XX-pseudo-male parent possessed lower sex reversal ratio at the same rearing temperature, and the proportion of female froglets from an XX-pseudo-male parent was more than 95% at low rearing temperature (15°C). Our findings suggest that R. dybowskii displays male heterogamety, and the 2 sex-linked molecular markers may have a guiding significance for the protection and utilization of R. dybowskii.     

水稻黑条矮缩病毒S6片段的序列多样性与进化分析

<正>0 引言植物RNA病毒在进化中具有快速变异的能力,有利于病毒扩大寄主范围甚至进化为新病毒[1]。突变和重组是植物RNA病毒变异和遗传多样性的共同进化因素,是群体基因变异的最初来源[2]。由灰飞虱传播的水稻黑条矮缩病毒(Rice black streaked dwarf virus, RBSDV) 属于呼肠孤病毒科(Reoviridae)斐济病毒属(Fijivirus),能引发玉米粗缩病和水稻黑条矮缩病。     

一起母牛采食马铃薯皮引起亚硝酸盐中毒误诊案的思考

为切实加强动物中毒性疾病的诊断处置能力,防止因诊断失误、处置不当导致动物死亡现象的发生。本文就一起母牛采食马铃薯皮引起亚硝酸盐中毒误诊案的诊断处置过程进行讨论分析,提出了相应的诊断处置意见,供广大兽医同仁在实际诊断过程中借鉴应用。     

Effect of compound salvae-dropping-pill on intracellular free calcium concentration of cultured rat myocardial cells in case of hypoxia and reoxygenation

AIM:To examine the effect of compound salvae-dropping-pill (CSDP) on intracellular free calcium in cultured rat myocardial cells subjected to hypoxia and reoxygenation.METHODS:The Fluo- 3/AM was applied to probe intracellular calcium concentration and the fluorescent intensity was detected using laser confocal microscopy technique.RESULTS:Fluorescent intensity in hypoxia plus CSDP group was significantly lower (1 217.78±312.07) than that of hypoxia group (1 509±508.48), and the Fluorescent intensity of hypoxia/reoxygenation plus CSDP group was also markedly lower (1 567.91±577.61) than that of hypoxia/reoxygenation group (1 617.60±477.53).CONCLUSION:The cultured rat myocardial cells could be effectively protected by administration of CSDP in case of hypoxia and reoxygenation through decreasing the intracellular calcium concentration.