Activation of repair pathways after neuronal DNA damage induced by bupivacaine

AIM To investigate the activation of related repair pathways after bupivacaine-induced neuronal DNA damage by cDNA gene screening. METHODS The bupivacaine-induced SH-SY5Y neuronal damage and DNA damage model was established. The technique of cDNA microplate array was used to screen the 21 important regulatory factors in the DNA damage repair pathway. Post-analysis of these differentially expressed repair genes for the repair pathway enrichment and distribution was performed. The data were analyzed by GraphPad Prism 6 statistical software to compare differences between groups. RESULTS The viability of SH-SY5Y cells treated with bupivacaine at different concentrations （detected by CCK-8 assay） showed that the IC₅₀ value of bupivacaine was 1.5 mmol/L. The comet assay related index （the comet tail） was increased （P<0.05）, the phosphorylation level of γH2AX protein was increased （P<0.05）, indicating that DNA damage in the SH-SY5Y cells was significantly aggravated after bupivacaine treatment. The results of cDNA microplate assay showed that compared withcontrol group, the differentially expressed genes after bupivacaine treatment were DNA-PKcs, PTEN, NTH1, RAD9, CSB, GADD45, XPD, XPC-HR23B and P53. The analysis showed that these repair genes were mainly concentrated in the following 3 repair mechanisms： base excision repair, nucleotide excision repair, and non-homologous reconstitution. CONCLUSION The repair genes differentially expressed after neuronal DNA damage caused by local anesthetics are mainly concentrated in the pathways of non-homologous end-joining, base excision repair and nucleotide excision repair.     

基于UHPLC-Q-TOF/MS的不同产地普洱生茶化学成分差异研究

为了更加全面地了解产地对普洱生茶品质与化学成分的影响,本研究选取来自临沧市、普洱市、西双版纳州三大产区12个茶山(自然村)的普洱生茶样品,采用超高效液相色谱-四级杆-飞行时间质谱(UHPLC-Q-TOF/MS)对不同产地普洱生茶的非挥发性代谢物表型进行了研究。结果表明:不同产地普洱生茶的化学成分在含量上具有较大差异,且具有明显的产地特征。采用主成分分析,可以对来自西双版纳自治州(包括勐腊县、勐海县、景洪市)、普洱市、临沧市3个地级行政区的普洱生茶进行有效区分,也可以对来自普洱茶产区的东南、西南、西北3个区域的普洱生茶进行有效区分。进一步鉴定了普洱生茶中79种主要成分,并对其在12个茶山(自然村)的普洱生茶中的含量分布,以及与不同产地普洱生茶滋味品质的关系进行了分析。本研究表明,基于UHPLC-Q-TOF/MS的茶叶非挥发性化学成分轮廓可以作为普洱生茶产地判别的依据。     

Polymorphisms of the <Emphasis Type="Italic">FT</Emphasis> gene as a tool to identify underground rhizome types of bamboos

The Flowering Locus T (FT)-like genes of angiosperms are highly conserved. The FT-encoded proteins include a phosphatidylethanolamine-binding domain that is involved in the control of the shoot apical meristem identity and flowering time. In the present study, FT genes were investigated in 20 bamboo species that are grouped into sympodial, mixed and scattered bamboos based on their morphology. All examined orthologous FT genes consisted of four exons and three introns. Their encoded protein sequences contained the critical amino acid residues Tyr85, Glu109, Leu128, Tyr134, Trp138, Arg139, Gln140 and Asn152, of which each possesses a biological function. The DNA sequences were rich in single nucleotide polymorphism (SNP) sites. The SNP frequency was 1 SNP/16.8 bp, and the nucleotide diversity (π) equaled 0.265. Some SNPs altered restriction enzyme sites or resulted in changes in amino acid contents. The correlation analysis showed that several SNPs were informative in relation to the underground rhizome types of bamboos. Therefore, FT polymorphisms could be used as a tool to identify the underground rhizome types of bamboos. The phylogenetic tree constructed based on the FT gene sequences showed that the obtained clustering was consistent with the underground rhizome types. The SNP markers developed in the present study will provide information on the genetic diversity of bamboos and they can aid taxonomic study as well.     

高蛋白优质大豆新品种濉科20的选育

濉科998×徐8313有性杂交、系谱法选育而成的濉科20,2012-2013年参加安徽省区试,每hm~2产量2857.0kg,比中黄13(CK)增产1.16%;2014年省生产试验,产量为2514.2kg,比对照增产6.05%。子粒蛋白质含量45.33%,蛋脂合计65.31%。抗SC3、SC7,生育期101d。该品种为非转基因大豆,具有蛋白质含量高、品质优良、生育期适中、综合抗性强、农艺性状优良等优点,2016年通过省审,适于江淮、淮北及相同生态区推广种植。     

木霉耐盐突变菌株的紫外诱变选育

为了获得高效耐盐木霉突变菌株,以盐碱土中分离的一株深绿木霉T-YM作为原始菌株进行紫外诱变处理,并筛选了突变菌株的最佳诱变条件,利用NaCl溶液模拟盐胁迫条件测定了突变菌株耐盐指标生长速率、产孢量和菌丝生长量。结果表明,与对照(野生型菌株)相比,当诱变时间为3 min时能够显著提高深绿木霉T-YM突变菌株菌落生长速度和产孢量,分别增加17.69%和28.82%;诱变时间为0.5 min和5 min时能够显著提高突变菌株菌丝生长量,分别增加42.22%和46.67%。利用10 mg·mL^-1 NaCl溶液模拟盐胁迫条件时,与对照相比突变菌株菌落生长速度、产孢量和菌丝干重整体高于野生型菌株,尤其当诱变时间为3 min时,不同浓度盐胁迫下其产孢量平均增加51.18%,诱变时间为0.5 min时其菌丝干重平均增加23.65%;NaCl胁迫(10 mg·mL^-1)下,诱变处理0.5 min获得的正突变菌株经传代接种培养后其耐盐性较为稳定。因此,紫外诱变可作为一种选育高效耐盐木霉突变菌株的有效方法。     

国内外核桃破壳技术与装备研究进展

破壳作为核桃产品深加工的重要环节,对整仁率的提高、核桃产业的収展和经济价值的提升具有重要作用。我国核桃产量的不断增加、核桃加工规模的逐步增大,对我国核桃破壳装备提出了新要求。为此,概述了国内外核桃破壳特性的研究迚展,归纳了核桃破壳技术与装备的研究成果,分析了相关技术装备的优缺点,旨在为我国核桃破壳装备的性能优化提供参考和依据。     

MATLAB在植物电信号降噪处理中的应用

植物电信号具有复杂性和时变非稳态特性,在获取有用信号前,需要对原始信号进行预处理,并将采集的信号严格地限制在有用的频段内。首先对信号进行低通滤波,以抑制高频噪声;然后采用陷波滤波器消除因测试环境中的有源设备而引起的50 Hz工频干扰。另外,采用小波降噪的方法处理采集的信号,更容易地分离出噪声或其他不需要的信息。通过MATLAB语言进行编程,设计数字滤波器,对信号进行预处理,并且用小波分析方法对信号进行分析,实现了降噪和去除干扰的目的,从而对植物电信号进行有效的分析。      

阳极溶出伏安法快速测定谷物中的镉含量

建立了一种阳极溶出伏安法快速测定谷物中的镉含量。样品经微波消解后,采用玻碳电极为工作电极,AgAgCl电极为参比电极,铂电极为对电极,测定谷物中镉含量,其检出限为0.4 μgL。采用标准加入法,平均回收率100%～104%;某一浓度样品同时测定6次,其变异系数2.43%。该方法稳定、快速和准确,可广泛用于粮食的安全事件应急监测。      

68.75%噁酮.锰锌水分散粒剂对柑橘黑点病的防治效果

柑橘黑点病(Diaporthe citri)是危害柑橘最严重的病害之一,每年需要喷施4-5次杀菌剂进行防治,为明确68.75%噁酮.锰锌WG对柑橘果实黑点病的防治效果,分别在浙江黄岩和常山进行了田间试验。结果发现黄岩试验点68.75%噁酮.锰锌WG 500倍全程5次对黑点病的防治效果为84.79%、常山试验点同样药剂800倍全程4次对黑点病的防治效果为74.10%,效果不亚于常规推荐的杀菌剂代森锰锌,说明68.75%噁酮.锰锌WG 500-800倍在柑橘花谢2/3后开始第一次用药,隔20-25天喷施一次,共用药4-5次,可有效防治柑橘黑点病。     

长沙市某县退出小反刍兽疫强制免疫暴发的定性风险评估

小反刍兽疫(PPR)是一种由小反刍兽疫病毒引起的疾病,主要感染山羊和绵羊。2021年为加快推进小反刍兽疫无疫区建设,实现小反刍兽疫非免疫无疫区建设标准,长沙市某县拟在本地开展小反刍兽疫退出强制免疫风险评估。通过对小反刍兽疫监测流调、危害识别、风险路径的确定和风险因素层级评估等方法进行的定性风险评估初步显示,在该地取消小反刍兽疫强制免疫后可能会发生小反刍兽疫的风险等级为中。在风险管理措施上,提出该地退出强制免疫应推迟一年,重点完善政策支持、加强监测排查、加强检疫监管、强化培训宣传等建议。