对如何提高蚕农科技素质的探讨

栽桑养蚕是我县广大农村经济发展的支柱产业和农民致富奔小康的重要途径.县委、县政府提出到2010年实现年产500万kg优质茧基地县,在建设社会主义新农村中发挥应有的作用.     

Allelopathic potential of watermelon tissues and root exudates

Allelopathic potential of watermelon in hydroponic culture was investigated using the continuous root exudates trapping system (CRETS). Laboratory bioassays and high-performance liquid chromatography (HPLC) were used for quantification of phytotoxins in different plant tissues and root exudates collected using XAD-4 resin. Seedling growth of both watermelon and lettuce was significantly inhibited by watermelon root exudates and the degree of inhibition increased with increasing concentration of exudates. Root exudates had inhibitory effects throughout the experiment and these were most pronounced during the period 41–50 days after transplanting. Inhibitory effects of various plant tissues were demonstrated using the bioassay tests. Frulic acid was the dominant component found using HPLC and the amounts of allelochemicals found in the plant extracts were correlated with the bioassay results using the Petri dish test. The results indicate that the allelopathic potential of watermelon may play an important role in the phenomenon referred to as ‘soil sickness’.     

白菜抗小菜蛾网室鉴定和离体鉴定方法比较

以8份白菜自交系材料为试材,分别利用网室鉴定和离体鉴定两种方法进行抗虫性鉴定。结果表明,两种鉴定方法的结果基本一致,材料间对小菜蛾抗性均表现极显著差异;在网室鉴定和离体鉴定中,599-3-7均表现高抗,519-3-3均表现中抗,而114-4-4和116-4-4都表现高感,部分材料的表现稍有差异;两种鉴定方法的相关系数为0.96,达到极显著水平;离体鉴定和网室鉴定相结合,能准确、全面地研究植物对小菜蛾的抗性及抗虫机理。     

基因芯片在百合无症病毒检测中的应用

设计百合无症病毒、植物18SrRNA基因的特异扩增引物和各种对照的探针，将探针固定在醛基化玻璃片基上，完成植物基因芯片制备。提取百合种球总RNA，经RT-PCR扩增相应区段并用Cy3dCTP进行标记，用标记的PCR产物与芯片杂交，扫描仪对杂交结果进行扫描，GenePixProd．0软件对杂交图像进行分析。结果从荷兰进口的百合种球中检测出百合无症病毒，证明了该实验研制的植物病毒基因芯片的准确性和灵敏性。     

试论宾馆、酒店动物性食品安全控制程序

动物产品是人类赖以生存的重要物质基础,如肉、蛋、禽、奶等动物产品是人类食物构成的重要组织部分,社会越发达,经济越发展,社会文明程度越高,人类对动物产品的依赖性越强,     

一起动物检疫行政不作为案的诉讼启示

1案例2004年12月10日,我市某区人民法院公开审理了某生猪屠宰企业诉动物检疫站不履行法定检疫职     

新阶段的新问题——关于当前畜牧产业发展的几点思考

当前畜牧业发展已经进入一个新阶段,规模化、集约化比重增加,畜产品数量与质量并重,畜牧业与整个社会和谐发展,成为这一阶段     

浅谈动物卫生监督机构行政处罚案件中的证据规则

动物卫生防疫监督机构依法承担着动物防疫、检疫与动物产品安全监管的行政执法重要职能。在动物卫生防疫行政处罚案件中,证明当事人的违法事实及行政处罚的合法性的核心是证据。根据我国行政处罚法及现行的动物卫生防疫监督机构行政处罚程序规定,可以作为行政处罚的     

Genetic heterogeneity of leucostoma species in michigan peach orchards

ABSTRACT The pathogens causing perennial canker of peach, Leucostoma spp., were characterized in Michigan orchards to identify which species and subgroups (cryptic species) were prevalent on a highly susceptible peach cultivar, Loring, and a less susceptible cultivar, Redhaven. Four hundred and three strains of Leucostoma were isolated from cankers in three southwest Michigan orchards where 'Loring' occurred adjacent to 'Redhaven' in side-by-side plots. Based on colony morphology and small nuclear rDNA size polymorphisms, three cryptic species were detected; 89% of the isolates were identified as L. persoonii phenetic group 1, 10% were L. cincta group 4, and 1% were L. persoonii group 2. Pathogen profiles differed significantly between cankers on small branches of 'Loring' and 'Redhaven', and between cankers on small branches and trunks. Of 1,232 random pairings among isolates of L. persoonii group 1, 95% were vegetatively incompatible. A minimum of 11 and a maximum of 17 maternal lines were identified based upon mitochondrial DNA restriction fragment length polymorphisms among 69 isolates of L. persoonii group 1 from one split-cultivar block of 72 trees. Vegetative compatibility loci were randomly associated with maternal lines. Evidence of clonality was absent in L. persoonii group 1, as no correlation occurred between these two sets of independent genetic markers and no genotype was over-represented. The number of cryptic species, the number of maternal lines, and the frequency of incompatibility within maternal lines indicate that considerable genetic variation exists within the Leucostoma populations in Michigan peach orchards, and that sexual recombination is common.     

不同培养基对香菇菌丝体酯酶同工酶的影响

本文应用垂直板聚丙烯酰胺凝胶电泳,对在不同培养基上生长的香菇菌丝体进行酯酶同工酶分析。结果表明,不同培养基会导致香菇菌丝体酯酶同工酶酶谱产生差异。