一种便携式农田小气候信息采集装置的设计

针对农田小气候监测的需要设计了一种便携式农田小气候信息采集装置,并对该装置系统结构、硬件结构及系统软件设计进行了详细介绍。实地调试结果证明,该装置具有较好的灵活性,便于携带,采集信息范围较广,完全能满足当前农业生产的需求,适用于农田、果园、草原等多种农业环境。     

荔枝FKBP16-2基因启动子的克隆与瞬时表达分析

前期研究中发现了一个果肉低表达而叶片中高表达的荔枝基因FKBP16-2。本文克隆了该基因1 578 bp的启动子片段并对其功能进行了初步分析,结果表明:荔枝FKBP16-2基因启动子序列中含有大量的TATA-box和CAAT-box保守元件,以及TCA-element,ARE,HSE,GCN4_motif,O2-site等各种转录调控相关的顺式作用元件。该启动子能驱动GUS基因在荔枝的花、叶、根、果皮以及种子中表达而在果肉中不表达,表达具有组织特异性。     

霜桑叶与野生植物资源组配保健茶方剂探析

为提升霜桑叶利用价值,拓宽桑叶应用领域,以承德当地选择几种常见野生菜用及药用植物为研究对象,根据其不同营养成分及功效分别与霜桑叶配伍,研制出具有预防感冒、清热解毒、降低血糖血压等不同功效的复方保健茶品。     

不同比例生物有机肥和复合肥混施对重庆地区烟叶农艺性状和品质的影响

为研究不同比例生物有机肥和复合肥混施对烟草农艺性状及品质的影响，采用田间小区试验，分别设置3种不同施肥处理，分析烟草不同生育期农艺性状、烟叶物理性状、烟叶外观等以及烟叶烟碱、总糖、还原糖等烟草品质指标。结果表明：单施复合肥处理(CK)前期烟草农艺性状优于不同比例生物有机肥和复合肥混施处理(T1和T2)，平顶期T1和T2的农艺性状则较CK组有明显优势；烟草中部叶片物理性状表现为T1处理最优，T2次之，CK最差；烟叶品质方面，T2处理烟叶外观质量有所改善，但其化学成分烟碱含量降低。烟叶品质总体表现为T2最优，     

Effect of AIR on Inflammatory Reaction Infected by Brucella

In order to investigate the effect of AIR on inflammatory reaction infected by Brucellamelitensis (16M), the AIR domain of Tecpr1 gene of murine macrophages RAW264.7 were knocked down (I-A), overexpressed (O-A) and reversed (OA-IA). Using the chlorine fluorescein (DCFH-DA) as a probe, we detected the variation of ROS production and mitochondria distribution by confocal laser scanning microscopy. We observed the expression changes of NLRP3, ASC and Caspase-1 by qRT-PCR and the expression changes of IL-18,IL-1β and Caspase-1 in host cells by ELISA. The results showed that 16M could stimulate RAW264.7 cells to produce ROS by time-dependent pathway, and I-A group and O-A group showed more abnormal accumulation of mitochondrial. The results of qRT-PCR and ELISA suggested that it had effect on the expression levels of NLRP3, ASC,Caspase-1 and IL-18, IL-1β and Caspase-1 in cells of different groups. Those results indicated that with AIR gene deletion, the release amount of ROS changed, mitochondrial clustered abnormally, and AIR was closely related to the activation of inflammasomes and induction of inflammatory reactions.     

果菜类蔬菜早熟性育种研究进展

早熟性是蔬菜作物育种的重要指标,对农业增产和农民增收意义重大。本文对果菜类蔬菜早熟性评价指标,早熟性相关农艺性状和早熟性育种方法三个方面的研究进展进行综述,并对基因工程辅助早熟育种进行了展望。     

黄瓜种质资源单瓜种子产量评价及其与种瓜性状的相关性研究

对不同生态类型的50份黄瓜种质资源的单瓜种子产量进行检测,并对单瓜种子产量与种瓜性状进行相关分析。结果表明,黄瓜单瓜种子产量在品种间存在一定的差异,变幅在0.5~7.9 g之间;筛选出单瓜种子产量在6.2 g以上的高产黄瓜种质资源5份,单瓜种子产量在1.9 g以下的低产黄瓜种质资源5份。黄瓜单瓜种子产量与7个性状相关,其影响顺序为:单瓜籽粒数>心腔横径>百粒重>单瓜重>种子长>种子宽>心腔纵径。通径分析表明,单瓜籽粒数和百粒重是影响黄瓜单瓜种子产量的主要因素,直接通径系数分别为0.908、0.363。此外,4种生态类型之间的单瓜种子产量差异不显著。     

Cloning and Protein Structure Analysis of Hypoxia Inducible Factor-1α Gene in Yak

In this study,hypoxia inducible factor-1α(HIF-1α) gene was cloned from yak spleen by RT-PCR,and it was divided into two sections for amplification.The sequence and protein structure were analyzed using related bioinformatics tools.The length of the coding region was 2 472 bp,which encoded 823 amino acids.Its molecule mass was 92.13 ku,and PI was 5.09.It displayed as a hydrophilic protein,whose secondary structure was mainly composed of random coil and α-helix.It had 69 phosphorylation sites and 4 N-glycosylation sites.Phylogenetic tree displayed that Maiwa yak,Bos grunniens,Bos mutus and Bos taurus gathered into one cluster.Maiwa yak HIF-1α gene was successfully cloned in this study,it provided a viable reference for further study of genetic characteristics of HIF-1α.     

Advances on Detection Methods for Residues of Nitrofurans in Food

Nitrofurans are broad-spectrum antibiotics which have been widely used in food animals to the prevention and treatment of gastrointestinal infections.Due to abuse of drugs by criminals,animals could produce drug-resistant strains,which reduces the role of antibiotics.Meanwhile,excessive drugs residues in food has carcinogenic,teratogenic and mutagenic effects to the human body.In this paper,the damage and the detection methods of the residues of the nitrofurans in food are surveyed.The main detection methods include high performance liquid chromatography,liquid chromatography-mass spectrometry,liquid chromatography tandem mass spectrometry,enzyme-linked immunoassay,colloidal gold immunoassay and fluorescence immunity analysis method.The research status of various detection methods are summarized and the suggestions for further research are put forward.It is expected to provide reference for future research.     

雾霾污染对旅游产业发展的时空动态影响及其规制研究——以长株潭城市群为例

【目的】随着美丽中国建设和三大攻坚战等战略的推进,旅游生态环境成为旅游科学研究的重要问题,研究雾霾污染对旅游产业发展的动态影响及其环境规制作用,促进旅游节能减排。【方法】本文以两型试验区长株潭城市群为例,构建关于旅游产业评价的集成指标体系,采用重心模型和回归模型计量方法,探究旅游产业和雾霾污染发展轨迹,考察旅游产业发展与雾霾污染之间空间动态关联性,分析雾霾对旅游产业发展影响程度,检验雾霾污染与其他自变量交互作用下旅游产业发展的主导要素,最后以环境规制为门槛变量进一步讨论雾霾污染对旅游产业发展的影响,【结果】1)旅游产业重心偏移方向由长沙向衡阳方向移动,雾霾重心偏移方向在常德和株洲两地之间往返,无显著移动规律,旅游产业发展与雾霾污染之间存在一定的空间动态关联性,且两者之间存在反向抑制作用。2)雾霾污染对旅游产业发展存在影响,就研究区域而言,增加1%的雾霾污染会减少3.3%的旅游产业发展,且影响旅游产业发展的主要因素是旅游人数和环境规制。3)以环境规制为门槛变量,发现雾霾污染随着环境规制类型的不同而对旅游产业发展产生不同影响,无环境规制下,雾霾污染对旅游产业负面影响指数为0.8%,对旅游产业发展影响显著;宽松型环境规制下,雾霾污染对旅游产业负面影响为0.2%,影响程度略小;严密型环境规制下,雾霾污染对旅游产业负面影响为0.1%,影响性最小。【结论】雾霾污染对旅游产业发展存在负面影响,但政府环境政策可有效缓解雾霾污染,因此在环境规制下,雾霾污染对旅游产业发展的负面影响减弱。