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11.
The antibody response and circulating antigen levels in bovine calves, infected experimentally with Fasciola gigantica, were monitored using enzyme-linked immunoelectrotransfer blot (EITB) and sandwich ELISA, respectively. By EITB, the infected calves' sera recognized the polypeptides in the range of 54-58 kDa as early as 2 weeks post-infection. By 12th week post-infection, the lower two polypeptides of 12 and 8 kDa had disappeared. In sandwich ELISA, the circulating 54 kDa and whole worm antigen of F. gigantica were detected in the sera samples of infected calves as early as 2 weeks post-infection and persisted until the end of experiment (26th week PI). The 54 kDa antigen of F. gigantica appears to be specific and possesses promising immunodiagnostic potential for early prepatent diagnosis of bovine fasciolosis.  相似文献   
12.
The selection of Jatropha based on morphological information and molecular markers is essential as it is more reliable and consistent. Hence, twelve Jatropha accessions from different geographical areas of India were screened for genetic diversity using 19 morphological traits and 21 ISSR primers. The analysis of morphological traits grouped the accessions into five clusters. The cluster I consisted of J. curcas (CJC 18), J. curcas (CJC 20), J. curcas (CJC 22), J. curcas (CJC21), and J. curcas (CJC 25), and contained the maximum number of accessions; clusters II and IV contained the minimum number of accessions. Among all the characters, the highest range was exhibited by plant height and the least value by the number of branches. The twenty-one ISSR primers generated 156 polymorphic alleles. The average number of ISSR alleles generated was 7.47 per primer. The ISSR primer UBC 884 was highly informative with the maximum of 12 alleles. The 12 genotypes were grouped into eight clusters. The cluster I contained the maximum number of accessions, namely J. curcas (CJC 18), J. curcas (CJC 20), J. curcas (CJC 22), J. curcas (CJC21), and J. curcas (CJC 25). The clusters II, III, IV, V, VI, VII, and VIII (J. tanjorensis, J. gossypiifolia, J. glandulifera, J. podagrica, J. ramanadensis J. villosa, and J. integerrima) contained the minimum number of accessions. Maximum diversity between J. villosa and J. integerrima was noticed and the least diversity between J. curcas (CJC21) and J. curcas (CJC 25) seen because the ISSR markers differentiated the Jatropha accession into a wide genetic diversity as compared to the morphological data. The species-specific diagnostic markers identified in the study such as 1000 bp alleles for J. glandulifera by the primer UBC 826 is suitable for discriminating species of Jatropha, and thus can be used for identifying a Jatropha species from any mixed population comprising other members of the Jatropha complex.  相似文献   
13.
An indirect enzyme-linked immunosorbent assay (ELISA) using 27 kDa glycoprotein of Fasciola gigantica has been evaluated for its potential use in the diagnosis of bovine fasciolosis. Following experimental infection of rabbits, F. gigantica infection-induced antibodies were isolated and later used as ligands in affinity chromatography for isolation of infection-induced antibody-specific proteins. Among the five infection-specific proteins isolated, a glycoprotein of 27 kDa was later isolated by second-step purification using concanavalin A matrix. In crossbred cattle receiving different doses of infection (100, 200 and 400 metacercariae), the anti-27 kDa antibodies were detected as early as the 2nd week post infection. No direct correlation between initial dose, antibody response and fluke establishment was recorded. No cross-reaction was noted with the sera of goats experimentally infected with Paramphistomum epiclitum. ELISA with the 27 kDa glycoprotein could be a feasible diagnostic tool for the early detection of bovine fasciolosis.  相似文献   
14.
Polymerase chain reaction (PCR) was used to detect Fasciola gigantica infection in the snail intermediate host. Fasciola specific primers amplified a 124 bp fragment in PCR when the genomic DNA isolated from F. gigantica infected Lymnaea auricularia snails was used as template. In addition to the 124 bp amplicon, a ladder of DNA fragments representing amplification of the 124 bp repetitive sequences was observed. Genomic DNA of the parasite was used as a positive control, which also gave an amplification of the 124 bp fragment. DNA isolated from non-infected snails was used as a negative control and no amplification of this sequence was observed. This technique is highly specific and sensitive and possesses fairly good prospects of its utility as an epidemiological tool for ascertaining the infectivity status in ubiquitous snail populations.  相似文献   
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