CO
2 concentration in a marine phytoplankton,
Nannochloropsis sp., culture medium was observed to consider whether or not CO
2 defficiency for growth occurs in the culture medium of a mass culture. In the first experiment, the
Nannochloropsis sp. culture was continued for 4–5 days and the CO
2 concentration was observed three times a day, namely, the initial, middle and end of the light period. While, in the second experiment, the CO
2 concentration was observed every 2–3 hr for 24 hr on the second day after inoculation. In the first experiment, the total CO
2 concentration decreased gradually throughout the culture period, repeating its decrease and increase in the light and dark periods, respectively. At the end of the light period of the third day after inoculation, the CO
2 concentration decreased to concentrations which could not be determined. In the second experiment, the CO
2 concentration decreased in the light period, and increased in the dark period. The increment in the dark period was faster at heavier aeration. It was considered that some kind of carbon source should be supplied to obtain high density of
Nannochloropsis sp. ealier under strong light intensity.
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