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11.
Ahimbisibwe John Bosco Inoue Kousuke Shibata Toshiyuki Aoki Takahiko 《Fisheries Science》2010,76(2):389-394
The effect of bleeding on white muscle quality in amberjack and red sea bream was evaluated by measuring ATP-related compounds,
volatile basic nitrogen (VBN), and the trimethylamine (TMA) content. The freshness was evaluated by the K value, and the degree of spoilage was elucidated by VBN and TMA. ATP was rapidly converted to ADP and AMP in the muscle and
IMP was the main product of ATP degradation during iced storage. For both species, the IMP content was higher in the muscles
of fish that were bled than in those unbled during iced storage. Conversely, the K value and the levels of hypoxanthine (Hx) and VBN were higher in the muscle tissue of unbled fish than in the bled tissue
of both species. Similarly, the TMA content was higher in the unbled muscle tissues of both species after a week of storage. 相似文献
12.
Seiji KANEMATSU Yumiko TAGA Toshiyuki MORIKAWA 《Journal of General Plant Pathology》2001,67(4):333-334
A virus whose coat protein gene had a high sequence homology with the coat protein gene of Olive latent virus 1 was isolated from diseased tulip in Toyama Prefecture.
Received 1 June 2001/ Accepted in revised form 1 August 2001 相似文献
13.
Hashizume K Takahashi T Shimizu M Todoroki J Shimada A Hirata M Sato T Ito A 《The Journal of reproduction and development》2003,49(1):45-53
In vitro cell culture is a convenient tool for studying cellular mechanisms. In the present study, production of matrix-metalloproteinases (MMPs) in bovine endometrial (containing both epithelial and stromal cells) monolayer cells was examined. Blastocysts attached to the endometrial cells in a monolayer culture were examined for their effects on MMP-2 production. Initial attachment of blastocysts to the monolayer inhibited MMP-2 production by endometrial cells. But once trophoblast cells began to migrate into the endometrial cell layer, MMP-2 production increased, and at the same time MMP-9 production also became evident in the medium. In order to understand how blastocysts affected MMP-2 production, we examined the effect of progesterone, estradiol, insulin-like growth factors (IGFs), tumor necrosis factors (TNFs), and interferon-tau (IFN-tau) supplementation. It was IFN-tau that inhibited the production of MMP-2. In addition, progesterone at a lower dose appeared to inhibit MMP-2 production. Both TNF-alpha and TNF-beta strongly stimulated the production of MMP-2 and MMP-9, whereas IGFs had no effect. Based on these findings, it appears that conceptus has the capacity to inhibit MMP activity. 相似文献
14.
Toshiyuki Usami Shu Ishigaki Hiroko Takashina Yuko Matsubara Yoshimiki Amemiya 《Journal of General Plant Pathology》2007,73(2):89-95
Japanese isolates of Verticillium dahliae, a causal agent of wilt disease in many plants, are classifiable into pathotypes based on their pathogenicity. Because these
pathotypes are morphologically indistinguishable, establishing a rapid identification method is very important for the control
of this pathogen in Japan. For cloning DNA fragments that are useful for identification and specific detection of V. dahliae pathotypes, we performed random amplified polymorphic DNA (RAPD) analyses using various isolates. One polymerase chain reaction
(PCR) product, E10-U48, was specific to isolates pathogenic to sweet pepper. The other product, B68-TV, was specific to race
1 of isolates pathogenic to tomato. The specificity of these sequences was confirmed by genomic Southern hybridization. Further
analyses revealed that the region peripheral to B68-TV obtained from the genomic DNA library includes the sequence specific
to all isolates pathogenic to tomato (races 1 and 2). Moreover, sequence tagged site (STS) primers designed from B68-TV and
its peripheral region showed race-specific and pathotype-specific amplification in a PCR assay. The probes and primers obtained
in this study are likely to be useful tools for the identification and specific detection of pathotypes and races of V. dahliae.
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession number AB095266. 相似文献
15.
For better understanding wood hardness, we developed a new hand-operated hardness tester that works with Brinell's method. With this tester we investigated the hardness distribution of wood minutely using a 2mm diameter ball tip. The following results were obtained: (1) On preliminary examination with medium-density fiberboard, we found that the value of Brinell's hardness decreased with the increase in the tip ball's diameter but that it was almost constant with the indenting velocity. (2) By using a small diameter tip, the difference of the hardness became clearer between earlywood and latewood. (3) With radial and tangential sections, we obtained hardness distribution patterns similar to those of wood grain. It seems that the hardness distribution reflects the distribution of density on wood surfaces. 相似文献
16.
Nobukazu SAISHU Kazutaka MORIMOTO Hiroshi YAMASATO Hiroichi OZAKI Toshiyuki MURASE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1037-1042
Thirty-eight Aerococcus viridans isolates were obtained from milk from
478 cows with clinical mastitis in a farm during the periods between November 2011 and
February 2012, and between December 2012 and March 2013. Additional isolates were obtained
from processed manure (a mixture of composted manure, straw and hydrated lime) and bedding
materials. The processed manure was later used to cover the floor of the stalls in barns
as bedding materials. The temperatures recorded in the composted and processed manure were
not as high as those generally observed during satisfactory composting. To reveal the
association of A. viridans in manure-related products with intramammary
infection in cows, isolates were characterized by their DNA fragment patterns as
determined by pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility
testing. Isolates obtained from milk, processed manure and bedding materials had identical
DNA fragment patterns. Antimicrobial susceptibilities were determined for 29 isolates from
milk, processed manure and bedding materials. Of these, 26 (89.7%) were resistant to
clindamycin, whereas virtually all the isolates were susceptible to 12 other
antimicrobials including cefalosporins that have been used to treat bovine mastitis in
Japan. In vitro, three A. viridans isolates from milk
and an isolate from processed manure survived for 3 hr in Good’s buffer (pH 9) at high
temperature (50°C). The results suggest that the processed manure and bedding materials in
this farm were possible sources of A. viridans that caused infection in
the cows with mastitis. 相似文献
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19.
Tetsuji Ota Tsuyoshi Kajisa Nobuya Mizoue Shigejiro Yoshida Gen Takao Yasumasa Hirata Naoyuki Furuya Takio Sano Raul Ponce-Hernandez Oumer S. Ahmed Heng Sokh Vuthy Ma Eriko Ito Jumpei Toriyama Yukako Monda Hideki Saito Yoshiyuki Kiyono Sophal Chann Nang Ket 《Journal of Forest Research》2015,20(6):484-492
20.
Nakamura T Yoshida A Komatsuzaki N Kawasumi T Shima J 《Journal of agricultural and food chemistry》2007,55(12):4871-4876
To investigate a sourdough-specific peptide, low molecular weight peptides were extracted from sourdough. The peptide fraction was subjected to two kinds of chromatography to separate the peptides. Reverse-phase chromatography of the peptide fraction in the sourdough showed certain specific peptides. The specific peptide fraction was further separated by gel filtration chromatography. Liquid chromatography tandem mass spectrometry analysis identified one of the peptides as VPFGVG (six-mer). This sequence was estimated to occur at the 287-292 position of a low molecular weight glutenin subunit. The peptide (designed as SDP1) was produced by proteases derived from wheat flour. SDP1 showed angiotensin-converting enzyme (ACE) inhibitory activity, and the 50% inhibitory peptide concentration (IC50) was 336 microM. It is possible that the SDP1 peptide partially confers ACE inhibitory activity in sourdough. 相似文献