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851.
852.
Parasitism of Toxocara canis larvae in Japanese quails by inoculation of the ascarid eggs. 总被引:1,自引:0,他引:1
S Nakamura T Sotoyama S Hayasaka Y Kameyama S Maruyama Y Katsube 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(5):865-872
The distribution of T. canis larvae and pathological changes caused by them were studied in Japanese quails orally inoculated with 1,500, 4,000 or 15,000 embryonated eggs. Larvae were distributed mainly in the liver and, to lesser extent, in the muscles, brain, eyes and other organs. The number of larvae varied from 7 to 3,346, and from 1 to 288 in the liver and muscles (breast and legs), respectively. A small number of larvae were also recovered from the heart, gizzard, brain and eyes. In the groups of quails inoculated with 4,000 or 15,000 eggs, small white foci were observed on the surface of the liver 6 or 12 hr after inoculation. Histopathological examinations revealed necrotic lesions, leukocytic infiltration, granuloma and nodular lesions. The pathological changes became more serious with the large size of inoculum and days after inoculation. 相似文献
853.
854.
A study of reproductive performance in pregnant, IL-2 receptor beta-chain overexpressed transgenic mice 总被引:5,自引:0,他引:5
Namba Y Hondo E Morimoto M Nakamura O Kusakabe K Ito M Saito S Sagara E Kiso Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(1):99-101
Relationships between female reproductive performance and uterine natural killer (uNK) cells were investigated in pregnant IL-2 receptor beta-chain overexpressed transgenic (Tg2Rbeta) mice. At 8 days of pregnancy, all fetuses were alive, suggesting that implantation normally occurred in these mice. However, 47% of fetuses were dead at 10 days of pregnancy and at 12 days all fetuses were resorbing, indicating that fetal loss progressed with the advance of pregnancy. The placenta of Tg2Rbeta mice gradually decreased in weight with the advance of pregnancy. At 10 days the placental labyrinth, decidua basalis, and metrial gland in Tg2Rbeta mice were poorly developed, and more uNK cells were found in Tg2Rbeta mice than in the control mice. We propose that Tg2RPbeta mice are the first and interesting model that uNK cells can cause abortion, to clarify the involvement of uNK cell function in female reproductive performance. 相似文献
855.
Kozaki M Nakamura Y Iguchi M Kano R Watanabe S Fujiwara K Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(1):1-4
The expression of cytokeratins and involucrin was analyzed to identify the skin cells which compose the epidermis of dogs. The distribution of cytokeratins and involucrin in normal dog skin was immunohistochemically examined with 27 commercial monoclonal antibodies for human use. Antibodies, No.4. OV-TL12/13, 35betaH11, 4.1.18, CAM5.2, NCL5D3, Ks.13.1, Ks.18.04, Ks.19.1, 170.2.]4 and Ks.20.8 stained hair follicles and/or the sweat gland duct, but not the epidermis. Antibodies, 34betaB4, AE3, 34betaE12. LP34, RCK102, MNF116, AE1, KLI, DE-K10 and DE-K13 reacted with every layer of the epidermis, hair follicles and the sweat gland duct. These results were similar to those reported in the human skin. No positive staining, however, could be detected in the epidermis, hair follicles and the sweat gland duct with commercial antibodies, 6B10, Ks.7.18, Mu146-uc, E3, RCK108 and involucrin. Therefore, immunohistochemical investigation with these commercial antibodies developed for human skin examination might be available for investigating the origin of skin tumors in dogs. 相似文献
856.
857.
Structure of thymic cysts in congenital lymph nodes-lacking mice 总被引:1,自引:0,他引:1
Nakamura Y Qu N Terayama H Naito M Yi SQ Moriyama H Itoh M 《Anatomia, histologia, embryologia》2008,37(2):126-130
The ALY (aly/aly) mouse, a mutant of the C57BL/6j strain, has a severe immunodeficiency because of immature development of the immune organs. Both lymph nodes and Peyer's patches are lacking and both the thymus and spleen are small. Previous microscopical observation of their thymus glands revealed the presence of an indistinct border between the cortex and medulla, the absence of Hassal's corpuscles and the reduction of the medullary epithelial cell population. However, other microscopical findings for these glands have not yet been reported. In the present study, we performed light and electron microscopical observation of the thymus and found the consistent presence of extremely irregular shaped cystic cavities lined by microvilli-bearing epithelium in the medulla. The cysts comprised ceca and did not open into adjacent capillaries, although they contained some lymphocytes and macrophages in their lumens. In the thymus glands of normal C57BL/6j mice, only some small cysts oval in shape could be inconspicuously found in the medulla. Therefore, the thymic cysts may normally regress during thymic development, however, in ALY mice, the cysts may remain because of the organ immaturity. 相似文献
858.
Hara Y Yamawaki H Shimada M Okada K Tanai T Ichikawa D Miyake K Kizaki K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(7):697-702
Concern has been growing about the cardiac toxicity of antimalarial drugs. Artemisinin, a unique type of antimalarial drug originating from a Chinese medicinal plant, has minimal adverse effects, but it has been reported to inhibit delayed rectifier potassium current, a voltage-gated potassium current. However, no studies have been published concerning the effect of artemisinin on ligand-gated potassium currents. Therefore, in the present study, we examined the influence of artemisinin on the acetylcholine receptor-operated potassium current (IK.ACh), a ligand-gated potassium current, in guinea pig atrial myocytes using a patch clamp technique. Artemisinin (1 to 300 microM) inhibited I(K.ACh) induced by extracellular application of both carbachol (1 microM) and adenosine (10 microM) and that induced by intracellular loading of GTPgammaS (100 microM) in a concentration-dependent manner. Artemisinin inhibited carbachol-induced, adenosine-induced, and GTPgammaS-activated IK.ACh within almost the same concentration range. In left atria, artemisinin (1 to 100 microM) partially reversed the shortening of action potential duration induced by carbachol in a concentration-dependent manner. Carbachol-induced negative inotropic action in left atria was also inhibited by artemisinin (10 to 300 microM). In conclusion, we suggest that the anticholinergic action of artemisinin is mediated through inhibition of IK.ACh via inhibition of the muscarinic potassium channel and/or associated GTP-binding proteins. 相似文献
859.
Asano M Tsubota T Komatsu T Katayama A Okano T Nakamura S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(4):433-435
The effect of anesthetizing with a 1:1 combination of tiletamine hydrochloride and zolazepam hydrochloride (TZ) was evaluated in 75 Japanese black bears. TZ was administered to 43 captive and 11 wild, 8 captives and 13 hibernating captive bears at the doses of approximately 9.0 mg/kg (usual dosage), 18.0 mg/kg (high dosage) and 5.0 mg/kg (low dosage), respectively. Sufficient anesthetic effects were achieved in all bears, and rectal temperatures, heart rates and respiratory rates did not change significantly during an hour handling. Complete blood cell examinations showed no abnormal data. A combination of TZ would be an efficient and safe drug for chemical immobilization of Japanese black bears. 相似文献
860.
Kobayashi Y Okuda H Nakamura K Sato G Itou T Carvalho AA Silva MV Mota CS Ito FH Sakai T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(11):1145-1154
To investigate the genetic characteristics of phosphoprotein (P) and matrix protein (M) genes of variable rabies virus (RV) prevalent in Brazil, the authors genetically characterized the P and M genes from 30 Brazilian RV field isolates. Phylogenetic analysis based on the P and M genes revealed the presence of six RV variants that consisted primarily of three insectivorous bats, the vampire bat, dog and fox in Brazil. Specific amino acid substitutions corresponding to these phylogenetic lineages were observed, with Asp(42) and Glu(62) in the P protein found to be characteristic of Brazilian chiroptera- and carnivora-related RVs, respectively. Amino acid sequence motifs predicted to associate with a viral function in the P and M proteins were conserved among Brazilian RV variants. 相似文献