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761.
Mellett AM Nakamura RK Bianco D 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2011,25(1):71-75
Background: A major cause of death in dogs with primary immune‐mediated hemolytic anemia (pIMHA) is thrombotic disease. Ultralow‐dose aspirin (ULDA) is commonly used to prevent thrombosis in dogs with pIMHA; however, the efficacy of antiplatelet agents in dogs with pIMHA is unknown. Hypothesis: The use of clopidogrel (CL), alone or in combination with ULDA, would improve survival to discharge and at 90 days without important adverse effects compared with ULDA alone in dogs with pIMHA treated with standard immunosuppressive therapy. Animals: Twenty‐four client‐owned dogs with pIMHA. Methods: Prospective, positive‐controlled, unmasked clinical trial with dogs randomized in 3 treatment groups to receive PO ULDA or CL or both. Results: There was no identifiable adverse reaction, evidence of hemorrhage, or increase in transfusion requirements associated with CL therapy, either alone or combined with ULDA, compared with ULDA alone. There was no significant difference between treatment groups with respect to survival to discharge and at 90 days. Conclusions and Clinical Importance: This study suggests that CL therapy, alone or in combination with ULDA, was safe and had similar short‐term survival compared with ULDA alone in a small group of dogs with pIMHA able to tolerate oral medications and treated with standard immunosuppressive treatment. 相似文献
762.
763.
Biological characterization of sulfoxaflor, a novel insecticide 总被引:1,自引:0,他引:1
Babcock JM Gerwick CB Huang JX Loso MR Nakamura G Nolting SP Rogers RB Sparks TC Thomas J Watson GB Zhu Y 《Pest management science》2011,67(3):328-334
BACKGROUND: The commercialization of new insecticides is important for ensuring that multiple effective product choices are available. In particular, new insecticides that exhibit high potency and lack insecticidal cross‐resistance are particularly useful in insecticide resistance management (IRM) programs. Sulfoxaflor possesses these characteristics and is the first compound under development from the novel sulfoxamine class of insecticides. RESULTS: In the laboratory, sulfoxaflor demonstrated high levels of insecticidal potency against a broad range of sap‐feeding insect species. The potency of sulfoxaflor was comparable with that of commercial products, including neonicotinoids, for the control of a wide range of aphids, whiteflies (Homoptera) and true bugs (Heteroptera). Sulfoxaflor performed equally well in the laboratory against both insecticide‐susceptible and insecticide‐resistant populations of sweetpotato whitefly, Bemisia tabaci Gennadius, and brown planthopper, Nilaparvata lugens (Stål), including populations resistant to the neonicotinoid insecticide imidacloprid. These laboratory efficacy trends were confirmed in field trials from multiple geographies and crops, and in populations of insects with histories of repeated exposure to insecticides. In particular, a sulfoxaflor use rate of 25 g ha?1 against cotton aphid (Aphis gossypii Glover) outperformed acetamiprid (25 g ha?1) and dicrotophos (560 g ha?1). Sulfoxaflor (50 g ha?1) provided a control of sweetpotato whitefly equivalent to that of acetamiprid (75 g ha?1) and imidacloprid (50 g ha?1) and better than that of thiamethoxam (50 g ha?1). CONCLUSION: The novel chemistry of sulfoxaflor, its unique biological spectrum of activity and its lack of cross‐resistance highlight the potential of sulfoxaflor as an important new tool for the control of sap‐feeding insect pests. Copyright © 2010 Society of Chemical Industry 相似文献
764.
Yuri Hirayama Rie Takishita Hiroyasu Misawa Kazuhiro Kikuchi Koji Misumi Sachiko Egawa Sawako Motoyama Yasunobu Hasuta Yoshiyuki Nakamura Yutaka Hashiyada 《Animal Science Journal》2020,91(1)
This study aimed to compare the efficiency of non‐surgical embryo transfer (ET) using a newly developed catheter, which enables transferring embryos into a proximal site of the uterus (mostly uterine body), and surgical ET of vitrified porcine embryos. In Experiment 1, the catheter was inserted into 12 gilts, with each half of the group allocated to skilled or novice operators. The time required for insertion into the uterus did not differ between skilled and novice operators (4 min 9 s and 4 min 6 s, respectively). In Experiment 2, 12 gilts were used as recipients for non‐surgical and surgical ET with vitrified embryos (n = 6, each). There was no significant difference in the rate of piglet production based on the number of transferred embryos between surgical and non‐surgical ET (25.8% vs. 15.4%, p = .098). The results suggest that non‐surgical ET catheter allowed for easy insertion and transfer of embryos without special training. Although the catheter is effective for deposition of embryos into the proximal site of uterus, the efficiency of piglet production is not enhanced compared with surgical ET. The ET method using this catheter, being labor‐saving and less‐invasive, may contribute to the improvement of ET in pigs. 相似文献
765.
Masamitsu Tsukamoto Tadashi Nakamura Hirohiko Kimura Hitoshi Nakayama 《Journal of Pesticide Science》2021,46(2):125
Herein, we provide a brief overview of the synthesis and applications of trifluoromethylpyridine (TFMP) and its derivatives in the agrochemical and pharmaceutical industries. Currently, the major use of TFMP derivatives is in the protection of crops from pests. Fluazifop-butyl was the first TFMP derivative introduced to the agrochemical market, and since then, more than 20 new TFMP-containing agrochemicals have acquired ISO common names. Several TFMP derivatives are also used in the pharmaceutical and veterinary industries; five pharmaceutical and two veterinary products containing the TFMP moiety have been granted market approval, and many candidates are currently undergoing clinical trials. The biological activities of TFMP derivatives are thought to be due to the combination of the unique physicochemical properties of the fluorine atom and the unique characteristics of the pyridine moiety. It is expected that many novel applications of TFMP will be discovered in the future. 相似文献
766.
Shigemura Y Nakaba M Shiratsuchi E Suyama M Yamada M Kiyono T Fukamizu K Park EY Nakamura Y Sato K 《Journal of agricultural and food chemistry》2012,60(20):5128-5133
Elastin hydrolysate has apparent beneficial effects, and the food-derived peptide prolyl-glycine (Pro-Gly) is present in human blood after oral ingestion. Following ingestion of elastin hydrolysate (10 g/60 kg body weight) by healthy human volunteers, peripheral blood was used to prepare plasma samples from which peptides were extracted by solid phase extraction and fractionated by size-exclusion chromatography (SEC). Peptides in the SEC fractions were derivatized with phenyl isothiocyanate (PITC) and resolved by reversed phase (RP)-HPLC. Pro-Gly was the major food-derived elastin peptide, reaching a maximum (18 μM) at 30 min after ingestion, and decreasing to approximately 20% at 4 h after ingestion. Finally, in cell culture, levels of Pro-Gly in the medium above 0.1 μg/mL significantly enhanced elastin synthesis of normal human dermal fibroblasts (NHDF) without affecting the rate of cell proliferation. 相似文献
767.
Bhuwan Rai Hiroko Takahashi Kenji Kato Yo-ichiro Sato Ikuo Nakamura 《Genetic Resources and Crop Evolution》2012,59(8):1713-1726
PolA1, a single-copy nuclear gene encoding the largest subunit of RNA polymerase I, comprises highly polymorphic intron 19 and nucleotide tag (Ntag) sequences. We analyzed these sequences in 42 accessions, which differed in ploidy, of Triticum–Aegilops and Hordeum species. The lengths of the intron 19 sequences were ca. 110?bp long in Triticum–Aegilops species, except in four Sitopsis species, Ae. longissima, Ae. searsii, Ae. sharonensis, Ae. speltoides, which had introns similar in length to those of Hordeum species, i.e., ca. 240?bp long. Phylogenetic analyses of the Ntag sequences showed that the four Sitopsis and remaining Triticum–Aegilops species were classified into two discrete Hordeum and Triticum clades, respectively. The A and D genome-specific Ntag sequences of polyploid wheats were highly homologous with those of T. urartu and Ae. tauschii, respectively. In Ae. bicornis, another Sitopsis species, two accessions had the short intron 19 and Triticum–type Ntag sequence, which were highly homologous with those of the B genome in polyploid wheats, whereas one accession contained the long intron 19 and Hordeum–type Ntag sequences. In contrast, partial sequence analyses revealed that the three accessions of Ae. bicornis shared highly homology to single-copy DMC1 and EF-G genes. The discrepancy between these results indicates that the Sitopsis species were probably established by hybrid speciation including ancient introgressive hybridization between progenitors of Triticum–Aegilops and Hordeum. Although many researchers have proposed Ae. speltoides as a candidate for the B genome donor, our data suggest the existence of diploid B genome species in the past that were responsible for the origin of both polyploid wheats and Sitopsis species, including Ae. speltoides. 相似文献
768.
Kitahashi T Ikawa S Sakamoto A Nomura Y Tsujiuchi T Shimizu K Sasabe S Park EY Nakamura Y Tsutsumi M Sato K 《Journal of agricultural and food chemistry》2012,60(4):940-945
A water extract of shark cartilage was fractionated into acidic and basic fractions by preparative isoelectric focusing on the basis of the amphoteric nature of samples. The acidic fraction was further fractionated into ethanol-soluble and -precipitate fractions. After the carcinogenesis treatment using N-nitrosobis(2-oxopropyl)amine, hamsters received a diet containing each fraction or purified chondroichin sulfate to give 0.4% (w/w) for 50 days. Only administration of the acidic ethanol-precipitate-fraction-containing diet significantly increased serum inhibitory activity against matrix metalloproteinase (MMP)-9 and reduced the number of adenocarcinomas in the pancreatic duct. The active fraction predominantly consisted of chondroichin sulfate-containing proteoglycan. However, the purified chondroichin sulfate had no significant activity. These results suggest that the protein moiety of the proteoglycan might be involved in the increase of serum inhibitory activity against MMP-9 and suppression of pancreatic carcinogenesis in hamster. 相似文献
769.
Jun‐ichi Shono Shohei Sakaguchi Takahiro Suzuki Mai‐Khoi Q. Do Wataru Mizunoya Mako Nakamura Yusuke Sato Mitsuhiro Furuse Koji Yamada Yoshihide Ikeuchi Ryuichi Tatsumi 《Animal Science Journal》2013,84(11):744-750
Muscle damage induces massive macrophage infiltration of the injury site, in which activated pro‐inflammatory and anti‐inflammatory phenotypes (currently classified as M1 and M2, respectively) have been documented as distinct functional populations predominant at different times after the conventional acute injury by intramuscular injection of snake venoms (cardiotoxin, notexin) or chemicals (bupivacaine hydrochloride, barium chloride). The present study employed a muscle‐crush injury model that may better reflect the physiologic damage and repair processes initiated by contusing a gastrocnemius muscle in the lower hind‐limb of adult mice with hemostat forceps, and examined the time‐course invasion of M1 and M2 macrophages during muscle regeneration by immunocytochemistry of CD197 and CD206 marker proteins. CD197‐positive M1 macrophages were observed exclusively at 1–4 days after crush followed by the alternative prevalence of CD206‐positive M2 at 7 days of myogenic differentiation, characterized by increasing levels of myogenin messenger RNA expression. Preliminary PCR analysis showed that M2 may produce hepatocyte growth factor (HGF) in culture, providing additional benefit to understanding that M2 populations actively promote regenerative myogenesis (muscle fiber repair) and moto‐neuritogenesis (re‐attachment of motoneuron terminals onto damaged fibers) through their time‐specific infiltration and release of growth factor at the injury site early in muscle regeneration. 相似文献
770.
Noto Fumikazu Maruyama Toshisuke Yoshida Masashi Hayase Yoshio Takimoto Hiroshi Nakamura Kimihito 《Paddy and Water Environment》2013,11(1-4):463-471
We investigate the effect of climate change on water resources as snow storage (WRSS) using a regional climate model with a 20-km mesh (MRI-RCM20), in the Tedori River basin of Japan. We make four main predictions. First, annual average temperature in Kanazawa is predicted to increase by 2.7 °C by 2081–2100, compared to the period 1981–2000. The smallest predicted temperature difference is 1.1 °C in August, and the largest is 3.4 °C in April and December. The annual average temperature is predicted to change from 14.5 to 17.2 °C. Second, annual average precipitation is predicted to increase by 3.8 %, and precipitation during the snowfall season (December–February) is predicted to decrease by 13.2 %, as a result of increasing temperatures. Third, average WRSS for the period 2001–2100 is predicted by degree-day and tank model analyses. Both the analyses show very similar results. The degree-day analysis shows 200 mm of average WRSS in 2100, and 477 mm in 2050. These values constitute 26 and 63 % of the WRSS value in 2000 (754 mm). The tank model analysis shows WRSS of 260 mm in 2100 and 523 mm in 2050, which are 33 and 67 % of the WRSS (785 mm) in 2000, respectively. Fourth, to examine the effect on irrigation water especially on the paddy paddling period, discharge is predicted and considered under the predicted average WRSS is 520 mm in 2050, 258 mm in 2100, and reaches a minimum of 41 mm in 2100. 相似文献