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101.
Satoshi Gotoda Nobuyoshi Takahashi Hirofumi Nakagawa Mitsuyuki Murakami Tomoko Takechi Tomozo Komura Toshiro Uchida Yasushi Takagi 《Pest management science》1998,52(4):309-320
A series of novel 6-(1,2,4-triazol-4-yl) chromone and -1-thiochromone (benzo[b]thiazin-4-one) derivatives was obtained by cyclisation via thiosemicarbazides which were prepared by reaction of hydrazines and the corresponding isothiocyanates. Their fungicidal activity was evaluated against the rice blast fungus Pyricularia oryzae. Of this series, 2,5,8-trimethyl-6-(1-propyl-5-thioxo-3-trifluoromethyl-1,2,4-triazol-4-yl) chromone, 6-(1-butyl-5-thioxo-3-trifluoromethyl-1,2,4-triazol-4-yl)-2,5,8-trimethylchromone, 6-(1-hexyl-3-methyl-5-thioxo-1,2,4-triazol-4-yl)-2,5,8-trimethylchromone and 6-(1-allyl-5-thioxo-3-trifluoromethyl-1,2,4-triazol-4-yl)-2,5,8-trimethylchromone were highly active (pEC50>6·0). Structure–activity relationship studies using the capacity factor k′ as a hydrophobicity index suggested that the log k′ optimum for 2,5,8-trimethyl-chromone and -1-thiochromone derivatives was around 1·0, equivalent to a log Pow value of c. 4·4. © 1998 SCI 相似文献
102.
Tomoko Ikeda Keiichi Nagata Hiroshi Honda Toshio Shono Toshio Narahashi 《Pest management science》1998,52(4):337-342
Sikimi plant (also known as Japanese star anise), Illicium anisatium, is toxic to mammals. Extracts of Sikimi were studied for their insecticidal activity against the larvae of mosquito, Culex quinquefasciatus, and for their mechanism of action on ion channels. Crude methanol extract and its ethyl acetate-soluble fractions were insecticidally active, with EC50 values of 63·0 μg ml-1 and 43·7 μg ml-1, respectively. The ethyl acetate-soluble fraction was perfused through the bathing solution and the current induced by a brief (10 ms) application of GABA by pressure ejection through pipette electrode was recorded by the whole-cell patch clamp technique. The extract suppressed GABA-induced currents irreversibly with an EC50 value of 0·42 μg ml-1. The time constant of current fitted to the single exponential function was shortened by the ethyl acetate-soluble fraction at concentrations ranging from 0·1 μg ml-1 to 10 μg ml-1 in a concentration-dependent manner. It was concluded that Sikimi extracts decreased the affinity of GABA for its binding site on the GABA receptor, thereby suppressing GABA-induced currents. © 1998 SCI 相似文献
103.
ABSTRACT: A pelagic bacterial community structure was examined in experimental intensive shrimp culture ponds that have a shrimp–mangrove complex aquaculture system, an extensive shrimp culture pond and a mangrove area in Thailand by denaturant gradient gel electrophoresis analysis of polymerase chain reaction amplified partial 16S rRNA genes. Bacterial community structure in the intensive shrimp culture ponds was distinguishable from that of the mangrove area. In the extensive shrimp culture pond, the bacterial community structure resembled that in the mangrove area, but bacterial abundance was as great as that in the intensive shrimp culture ponds. Among the intensive shrimp culture ponds, the bacterial community structure was different between a closed culture system and a shrimp–mangrove complex culture system. Moreover, the bacterial community structure in mangrove planted ponds was close to those in the intensive shrimp culture ponds when shrimp culture was conducted, but it was close to those in the mangrove areas without shrimp culture. These results suggest that intensive shrimp culture with shrimp feed input affects the bacterial community structures in pond water. 相似文献
104.
Shinji SASAZAKI Tomoko HINENOYA Daisuke FUJIMA Shinichi KIKUCHI Akira FUJIWARA Hideyuki MANNEN 《Animal Science Journal》2006,77(1):42-46
In our previous study, a Kobe‐NIBS Japanese quail (KNQ) linkage map was constructed mainly using amplified fragment length polymorphism (AFLP) markers. In order to compare chicken and quail chromosomes, we developed expressed sequence tag (EST) markers derived from cDNA‐AFLP fragments and localized these markers on the linkage map. Using a total of 128 AFLP primer combinations, 24 polymorphic bands were obtained between a neurofilament‐deficient mutant quail line male and a muscular disorder quail line female, which were the parents of the KNQ resource family. Nine of the 24 markers were mapped by linkage analysis. These markers were mapped to seven linkage groups, namely 1, 2, 3, 6, 8, 15 and 42. A subsequent homology search using chicken genome sequences strongly suggests that these linkage groups correspond with chicken chromosomes 1, 2, 3, 5, 15, 23 and 26. 相似文献
105.
106.
Iwata A Yamamoto A Fujino M Sato I Hosokawa-Kanai T Tuchiya K Ishihama A Sokawa Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(6):721-724
Most animal cells that are exposed to interferon (IFN) experience an increase in the activity of 2', 5'-oligoadenylate synthetase (OAS), which is an important effector of IFN's antiviral action. OAS activity has been widely used in clinical chemistry as an indicator of IFN activity. In this study, we found that OAS activity in canine serum is 46.0 +/- 40.4 nmol/dl/hr, which is 10- to 100-fold higher than in other animals such as the cat (1.9 +/- 2.1), rabbit (4.0 +/- 1.1), and guinea pig (0.3 +/- 0.6). The canine OAS protein was detected by Western blotting using a 68M-10 monoclonal anti-murine OAS antibody, and was found to be composed of at least three distinct molecular species of p40 class OAS. Among these, the 40 and 42 kDa components were determined to be the major species in serum and fibroblast cell lines, respectively. 相似文献
107.
Seki Y Seimiya YM Yaegashi G Kumagai S Sentsui H Nishimori T Ishihara R 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(12):1503-1508
During the period from 2001 to the following year, progenital diseases had been epidemic among the draft stallions and mares pastured together in Iwate Prefecture, the northeastern district of Japan. A stallion and 8 of 31 mares were affected in 2001, and 1 of 2 stallions and 10 of 36 mares in 2002. The clinical symptoms consisted of the formation of papules, pustules, ulcers and scabs on the progenital skin and mucosa in stallions and mares. In 2002, Equine herpesvirus 3 (EHV3) was isolated from 2 mares and the glycoprotein G gene of the virus detected from a stallion and 4 mares by polymerase chain reaction. Serum neutralizing tests showed that 12 of 38 horses, 10 clinically and 2 subclinically affected, changed to be positive for the EHV3 antibody. The results suggest that the horses were affected with equine coital exanthema (ECE) through coitus. Five mares with the antibody at the pre-pastured period may have been the possible origins of EHV3 infection in 2002, although the exact origin in 2001 remains unknown. The artificial insemination was performed for the prevention of ECE spreading through coitus on the pasture in 2003. There was no epidemic of the disease in 31 mares, although 3 mares with the antibody at the pre-pastured period showed the significant increase in the titers during the pastured period. 相似文献
108.
Koie H Shibuya H Sato T Sato A Nawa K Nawa Y Kitagawa M Sakai M Takahashi T Yamaya Y Yamato O Watari T Tokuriki M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1453-1456
A castrated male border collie 23 months of age weighing 19.4 kg was referred to the Animal Medical Center of Nihon University with complaints of visual disturbance and behavioral abnormality, hyperacusis and morbid fear. The MRI examination revealed the slight dilated cerebral sulci and cerebellar fissures and left ventricular enlargement. This is the first report of MRI findings of canine neuronal ceroid lipofuscinosis. 相似文献
109.
110.