rep-PCR法对稻瘟病菌有性后代随机群体的评价

构建稻瘟病菌有性后代随机群体是研究目的基因遗传特点，乃至克隆目的基因的重要基础，但是在建立有性后代随机群体时，可能会有无性后代污染。采用先分离单个子囊，待其长成菌落并产生分生孢子后，再分离单个萌发的分生孢子的方法构建稻瘟病菌有性后代随机群体。利用基于重复DNA序列Pot2的rep-PCR方法对分离得到的有性后代进行DNA指纹分析，并评价构建的稻瘟病菌有性后代群体的质量。结果表明，构建的有性后代随机群体共包含176个后代。每个后代个体的DNA指纹图谱均与两亲本的DNA指纹图谱不一致；有性后代之间的DNA指纹图谱也彼此不一样，说明得到的有性后代个体都是由子囊孢子萌发形成的，且分离自不同的子囊。GUY11的rep-PCR扩增片段中的5个特异性片段的分离比例也都符合1:1的期望值，是按单个位点标记分离的，与已知的结果相吻合，说明有性后代随机群体的质量较高，不存在偏离现象，是一个较为理想的群体。同时，研究结果还得出基于Pot2的rep-PCR方法可以用来快速评价稻瘟病菌有性后代群体的质量。     

Analysis of Application of Information in Mid - mini Enterprises on Condition of Mass- economy

The group of Mid - mini enterprises under mass - economy is the intermediate organization built up by specialized division of work and cooperation. This kind of organization is flexible in operation with high efficiency, but difficult in development of new technology. On the basis of analyzing the character and deficiency of the mid - mini enterprises, in this paper, through providing a framework of information service platform for the mid - mini enterprises to enhance their creative ability and complex competition. The construction and operational strategy of such platform are investigated. Finally a successful case study is given.     

Mathematical Medel for Heat Transfer on Solididing Ladle of Hot Metal

In this paper,solidifinG ladle of hot metal has been studied from the point ofview of heat transfer.A mathematical model,which is applied to predict the shape and the thicknessof solidifing layer,has been developed. The theoretical calculated results agree with the appliedresults in preduction. The effects of various paramenters,such as the circulating times, the inner-surface temporature of ladle and the different refractories,on the shape and the thickness ofsolidifing layer have been discussed.These are contributed to reduce the solidifing ladle of hot metal.     

A Thermodynamic Criteria for Evaluation of Heat Exchanger Performance

The concept of loss rate of available energy was introduced for evaluating heat exchanger performance. At the same time, the general expression of loss rate of available energy was obtained for heat exchangers of several typical. And NL criteria was recommended for evaluation of the loss rate of available energy of heat exchanger. Then. the influences of inlet temperatures ratio, heat capacity ratio of fluids, the number of heat transfer units and flow patterns of heat exchanger on ,VL criteria were also discussed.     

Research on Dynamics of Installation Way for Active Isolation Actuator on Flexible Foundations

The dynamic characteristic of active isolation atuator on flexible foundations was studied under the installation of parallel structure and foundation cancellation.The influencing relationships between active isolation force of the different installation ways and passive isolation system and flexible foundation were analyzed and compared.Scientific basis for selecting installation ways of active isolator is provided in this paper.     

The Control Strategy for the Excitation System of Asynchronized Turbogenerators

In this paper, the excitation principle and operating characteristics ofasynchronized turbogenerators are analyzed,and adaptive tracing iterative control strategy based on state identification is proposed. In order to apply this control scheme to practice engineering, some primary elements are analyzed and presented for constituting dual-channel automatic excitation control system of asynchronized turbogenerators. Finally, the paper presents the principle structure charts about excitation control system of asynchroized turbogenerators.     

Load dynamic matching of supply and demand side based on the utilization of urban sewage heat energy

Flowing dynamic characteristics and its temperature dynamic characteristics of a three-level sewage pipe network in Chongqing were analyzed. A mall's building whole year load was simulated. In the adverse condition of wastewater flow, the market construction peak discharge heat was compared with the sewage heat load, the balance between the most adverse flow and the building demand was found. Explored supply and demand matching method between urban sewage and city building load in flow adverse condition to take full advantage of the urban sewage cold and hot resources. the composite system was adeptcd to make up the most unfavorable flow time of sewage average heat load and the construction load difference; it also puts forward principle of load sharing and control strategy aimed at compound system.     

直墙拱形巷道围岩应力场分析

为得出直墙拱形巷道围岩应力分布规律,应用复变函数弹性理论推导了直墙拱形巷道围岩应力分布的解析表达式。对直墙拱形巷道边界的围岩应力和巷道水平线方向的围岩应力分布规律进行分析,并考虑直墙拱形巷道断面高宽比和侧压系数对其影响规律。研究表明：在不同巷道断面高宽比、侧压系数下,直墙拱形巷道围岩应力集中区域主要集中在直墙底部底角处、拱形顶板中点附近和底板中部3个位置。不同巷道断面高宽比下,直墙拱形巷道沿水平线的应力分布规律基本相同。侧压系数大于1时,采用巷道断面高宽比小于1较有利于巷道稳定;侧压系数小于等于1时,采用巷道断面高宽比大于1较有利于巷道稳定。     

实用型荔枝虫害诊断专家系统设计方法研究 实用型荔枝虫害诊断专家系统设计方法研究

针对大型检索表为表示的专家知识体系，研究提出了一套可重用的、实用性强的系统设计方法，建立了荔枝虫害诊断专家系统（LDS）。经生产示范应用表明：实现了人机操作界面直观、明了，降低了客户端的专业要求，提高了系统的实用性。还对系统所构造的人机界面、知识存储结构设计和控制策略给出实践示例。