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71.
AIM: To investigate the mechanisms of transdifferentiation of renal tubular epithelial cells by the observation of serial activation of embryonic pax2 and WT1 genes in chronic renal failure model of 5/6 nephrectomized injury. METHODS: Embryo of mice, cultured renal tubule cells and chronic renal failure rat model of 5/6 nephrectomized injury were established. The expressions of paired Box gene (pax2) and Willm’s tumor gene (WT1), as well as α-smooth muscle actin (α-SMA), a phenotype of mesenchymal cells, were detected by RT-PCR and immunohistochemistry techniques. RESULTS: (1) Expressions of pax2 and WT1 mRNA began at 11.5 d and 14 d of embryo and increased gradually, and expression in trace quantity at 2 weeks after birth. Immunohistochemistry analysis revealed that WT1 only expressed in the glomerular podocytes and expressions of pax2 and WT1 in adult renal tubular cells were negative. (2) Deno-expression of pax2 and WT1 in some tubular cells appeared at week 2 and peaked at week 4 in 5/6 nephrectomized rats, both showing a same trend of expression. However, pax2 showed another peak at week 10 afterwards. (3) Re-expressions of pax2 and WT1 in TEC at 0.5 and 24 h after treated with IL-1α (10 μg/L) or AngII (10-9 mol/L) were observed respectively, followed by upregulation of α-SMA expression, and mesenchymal cells characters were shown. The effects were inhibited by Ang II receptor 2 antagonist and WT1 antibody, respectively. CONCLUSION: Adult renal tubule cells acquire re-activation of embryonic pax2 and WT1 genes and phenotypes of mesenchymes when challenge with certain injuries. Deno-expression of pax2 and WT1 genes closely relates with high concentration of bioactive facors, such as AngII and IL-1, which are involved in the mechanisms of renal tubule cells transdifferentiation. 相似文献
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73.
xPC环境下CMAC NN在淀粉生产线液位控制中的应用 总被引:1,自引:1,他引:0
在分析CMAC神经网络的基础上,提出了利用自适应CMAC神经网络来调节淀粉生产线中乳液的液位.通过对淀粉生产线中某一液位回路进行数学建模,然后对常规PID和自适应CMAC神经网络两种控制器进行了设计与仿真.仿真结果表明,自适应CMAC神经网络在控制效果上明显优于常规PID,并且具有很高的鲁棒性.在Matlab实时开发环境xPC Target下建立了液位实时控制系统,采用快速原型控制方式,具有系统组建方便、成本低、开发周期短等特点,该系统可实现精确的过程控制. 相似文献
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75.
介绍了一种利用非接触式速度测量装置驱动排种器的工作原理.该装置用前后两个光电传感器来探测路面的反射光信号,根据两路信号的时间差得到相对速度,并用该测速装置来驱动步进电机,步进电机带动排种器转动,以达到排种和速度同步的目的.此装置可以改善现有播种机靠地轮驱动时由于打滑造成的排种不均匀的问题,使排种更加均匀化. 相似文献
76.
Clostridium butyrium CB2 isolated from chickens was tested as a potential fish probiotic in the Chinese drum, Miichthys miiuy . Fish were fed live (CB), dead CB2 (D-CB) cells (108 cells g−1 ) or spent culture supernatant (SCS), for 30 days and challenged with Vibrio anguillarum or Aeromonas hydrophila . Survival was higher in both the CB and the CB-D fed groups, but the SCS group was not significantly different from the control. After feeding live or dead CB2 cells, there was increase in phagocytic activity of the head kidney macrophages, the lysozyme activity of serum and gut mucosa and immunoglobulin (Ig) level. The SCS group showed no obvious change in immune parameters. The results suggest that live or dead Clostridium butyrium CB2 has an immunomodulatory effect on fish. 相似文献
77.
对麻竹笋用林进行施肥试验研究,分析得知,鸡粪、复合肥、配方肥3种肥料对竹笋产量和经济纯收入有显著影响,均与竹笋产量、经济纯收入呈抛物线型关系,竹笋产量、经济纯收入随施肥量增加呈现先上升后下降的趋势;3种肥料的影响程度顺序依次为:配方施肥、鸡粪、复合肥,配方施肥的最佳施肥方案为鸡400+复60+硫酸锌0.9。 相似文献
78.
莰酮对蚜虫生物活性的研究 总被引:8,自引:0,他引:8
进行了樟树(CinnamomumcamphoraL.)中的莰酮(CamphorC10H160)对蚜虫的触杀、熏蒸毒力和拒食活性研究。结果表明,处理后24h莰酮对菜缢管蚜、桃蚜和棉蚜触杀作用的LD50分别为3.12×10-3,2.04×10-3,0.68×10-3 ng/头,熏蒸作用的LC50分别为3.44,2.56,1.06mg/L,拒食作用的AFC50分别为3.34,2.98,1.32mg/L。经回归和相关分析,处理浓度与作用效果相关性均达到极显著或显著水平。莰酮在田间防治菜缢管蚜的效果显著。 相似文献
79.
长期施肥对木薯农艺性状、鲜薯产量和淀粉质量分数的影响 总被引:8,自引:0,他引:8
1992—2003年12a间,对2个木薯品种(华南205和华南124),进行氮、磷、钾和火烧土等4种肥料各4个不同施肥水平组成共16个施肥配方的试验,研究了长期施肥对木薯农艺性状、鲜薯产量和淀粉质量分数的影响。结果表明:淀粉质量分数与收获指数呈显著正相关,鲜薯产量与株高、茎径及单株结薯数呈极显著正相关,单株结薯数与株高、茎径呈极显著正相关,株高与茎径呈极显著正相关。并列出它们的相关回归方程。推荐木薯最佳N,P2O5,K2O的施肥质量配比为2~4:1:2~4,其中,N,P2O5,K2O的施肥质量配比在连作初期以2:1:2为佳,而连作后期则以逐渐过度为4:1:4为佳。 相似文献
80.
A numerical model for analyzing dynamic properties of a net-cage system exposed in the open sea is proposed. The model is based on a lumped-mass method. In this model, the mooring lines are divided into linear elements and the net cage divided into several plane surface elements. The interconnected points or corners are called nodes or lumped-mass centers. The external force is calculated on each element and then equally distributed to its nodes. By adding the contributed forces from the neighboring elements, a system of motion equations for nodes is formed. The volume reduction coefficient of a net cage is estimated by the ratio of minimum volume of net cage during fluid and structure interaction to the original volume. In general, the numerical results are in good agreements with the experimental data. However, the results also show that if the Reynolds number is lower than the suggested range of 1400–1800, the numerical model may underestimate the environmental forces on a net-cage system. 相似文献