旱、盐胁迫下黄芪种子萌发及其对水杨酸的响应

通过测定不同浓度聚乙二醇(PEG)和NaCl胁迫下膜荚黄芪(Astragalus membranaceus)和蒙古黄芪(A.membranaceus var.mongholicus)种子的最终发芽率、发芽势、简化活力指数、苗长、根长、苗干重、根干重等指标,以及不同施用方式和浓度的水杨酸(SA)对重度PEG和NaCl胁迫下两种黄芪种子最终发芽率的影响,旨在研究两种黄芪种子对PEG和NaCl胁迫响应方式的异同及SA对两种黄芪种子在重度PEG和NaCl胁迫下保护效果的差异。结果表明,低浓度PEG和NaCl只能促进膜荚黄芪种子萌发。在-0.5 MPa PEG和-0.7 MPa NaCl处理下,蒙古黄芪种子的活力指数显著优于膜荚黄芪种子(P0.05)。SA浸种能促进重度PEG和NaCl胁迫下两种黄芪种子的最终发芽率。蒙古黄芪种子的耐胁迫性高于膜荚黄芪种子。两种黄芪种子对SA的响应方式有区别。SA浸种处理优于拌种处理。     

饲用荞麦在畜牧业中的应用与研究

荞麦(Fagopyrum esulentum)原产于中国,其栽培种甜荞和苦荞在世界各地已被广泛种植,我国西南地区蕴含着丰富的野生荞麦资源。荞麦营养丰富,除含有常见的蛋白质、纤维素和糖类外,还富含芦丁等抗氧化物质,具有耐瘠薄、抗病虫害、适应性强、生长迅速等优点,特别是一些野生种叶片大、叶量多,更适合于刈割,这些优点使其成为一种很具有发展潜力的优质牧草资源。但目前荞麦的饲用价值仍未得到充分的发掘与利用。本文着重介绍了荞麦在我国的栽培、饲用历史和现状,结合荞麦饲用于猪、反刍动物及家禽等方面的研究进展,分析了荞麦在饲用化发展中将面临的问题,并对其应用前景进行了评价与展望。     

对《一起未经兽医执业注册从事动物诊疗活动案的查处与思考》的商榷

加强对执业兽医的管理,有助于规范动物诊疗监管秩序和维护公共卫生安全。本文针对一起未经兽医执业注册从事动物诊疗活动案,从执法对象、罚没违法所得的认定以及法律适用等方面进行了辨析,围绕案件处置提出了不同观点。对相关法律条文的实用性和衔接性提出了思考,如法律法规可操作性不强,条款设置存在缺陷,对法律适用的解释不明确等,以期为法律法规的完善及执法实践提供参考。     

湖南省长沙市羊布鲁氏菌病基线调查

为了解湖南省长沙市布鲁氏菌病流行现状,对奶羊养殖场(户)和种羊场采用普查策略,对其他羊养殖场(户)采用两阶抽样策略,选择羊群并选定群体中个体,采集血样进行布鲁氏菌抗体检测。同时,通过问卷调查和查阅资料等方式,分析布鲁氏菌病感染和传播风险。结果显示:全市羊布鲁氏菌病表观场群流行率为1.54%(95%CI:0.42%~3.91%),表观个体流行率为0.60%(95%CI:0.43%~0.80%);4个阳性群集中分布于长沙县和宁乡县;和以往监测数据相比,长沙市的羊群布鲁氏菌病流行率和人间发病率均呈下降趋势,说明前期的防治策略取得积极成效。总体来说,长沙市布鲁氏菌病流行率较低,疫情处于稳定控制状态,因而可以分区域逐步实现全市布鲁氏菌病净化。     

The localization and expression of epidermal growth factor and epidermal growth factor receptor in bovine ovary during oestrous cycle

Epidermal growth factor (EGF) is one of the important regulatory factors of EGF family. EGF has been indicated to effectively inhibit the apoptosis of follicular cells, to promote the proliferation of granulosa cells and the maturation of oocytes, and to induce ovulation process via binding to epidermal growth factor receptor (EGFR). However, little is known about the distribution and expression of EGF and EGFR in cattle ovary especially during oestrous cycle. In this study, the localization and expression rule of EGF and EGFR in cattle ovaries of follicular phase and luteal phase at different time points in oestrous cycle were investigated by using IHC and real-time qPCR. The results showed that EGF and EGFR in cattle ovary were mainly expressed in granulosa cells, cumulus cells, oocytes, zona pellucida, follicular fluid and theca folliculi externa of follicles. The protein and mRNA expression of EGF/EGFR in follicles changed regularly with the follicular growth wave both in follicular and in luteal phase ovaries. In follicular phase ovaries, the protein expression of EGF and EGFR was higher in antral follicles than that of those in other follicles during follicular growth stage, and the mRNA expression of EGFR was also increased in stage of dominant follicle selection. However, in luteal phase ovaries, the growth of follicles was impeded during corpus luteum development under the action of progesterone secreted by granular lutein cell. The mRNA and protein expressions of EGF and EGFR in ovarian follicles during oestrous cycle indicate that they play a role in promoting follicular development in follicular growth waves and mediating the selection process of dominant follicles.     

多主体合作博弈视角下林业专业合作社盈余利益分配研究

盈余利益分配机制是否合理、完善,对林业专业合作社能否实现成功运转至关重要。文章基于林业专业合作社各利益主体在盈余利益分配上是一个合作博弈的过程,针对传统Shapley值法只考虑贡献率的特点,考虑到林农、投资者、经营者与公司承担风险性大小的差异,利用层次分析法提出改进的Shapely值法,并通过具体算例对改进Shapely值法进行了应用。     

利用蒸发冷却空调开展控温储粮试验

在拱板式高大平房仓的拱板间安装蒸发冷却空调开展控温储粮技术,解决拱板间的积热,以达到延缓仓内温度和上层粮温上升速度的目的。蒸发冷却空调主要利用水泵抽取水槽内的水,通过分水器均匀分布到特殊纤维制成的湿帘组件上,而大流量轴流风机的转动令仓外空气以极高速度穿过湿帘组件将湿气散发到空间,以增加空气中的湿度,通过正压促使空气流动,从而达到换气降温的效果。试验结果表明,利用低功率蒸发冷却空调,可以有效排除拱板间的积热,实现换气降温、节能、环保、绿色储粮的目的。     

1～21日龄艾维茵肉鸡净能需要量研究

本试验在实测饲粮玉米和豆粕净能(NE)值的基础上,研究1～21日龄艾维茵肉鸡净能需要量.选用1日龄的艾维茵肉公鸡250只,随机分为5组,每组5个重复,每个重复10只鸡,饲粮净能水平分别为8.97、9.25、9.54、9.83、10.11 MJ/kg,考察不同净能水平对1～21日龄艾维茵肉鸡生长性能和氮代谢的影响.结果表...