Isolation and characterization of alpha 1-acid glycoprotein from horses, and its evaluation as an acute-phase reactive protein in horses.

Equine alpha 1-acid glycoprotein (alpha 1AG) was isolated from equine serum by successive ammonium precipitation, anion- and cation-exchange chromatographies, and gel filtration. Purified equine alpha 1AG had a molecular weight of 46,000 +/- 1,000, and contained 31.4% carbohydrate. Gel isoelectric focusing revealed an isoelectric point range of 2.8 to 3.7. With immunoelectrophoresis, it was found that alpha 1AG migrated to the alpha 1-globulin region. Single radial immunodiffusion was used for quantitative measurement of alpha 1AG in equine serum. In clinically normal foals, serum alpha 1AG was undetectable (less than or equal to 20 micrograms/ml) in less than or equal to 7-day-old foals, but was detected by 14 days. The alpha 1AG concentration (mean +/- SD) increased to reach mean adult values of 99.23 +/- 26.90 micrograms/ml by 1 year of age. The alpha 1AG concentration in pregnant mares decreased at 2 to 3 months before parturition, then gradually increased until 1 day after parturition, when a brief decrease was observed. The concentration increased again at 2 weeks after foaling, then a decrease was observed, after which the alpha 1AG concentration increased again by 2 to 4 months after parturition. The concentration of serum alpha 1AG quickly rose to peak values 2 to 3 days after castration and jejunojejunostomy in adult horses, returning to baseline values by 14 to 28 days after surgery. The alpha 1AG was concluded to be an acute-phase reactive protein in horses.     

Fire protection of a laminated veneer lumber joint by wood carbon phenolic spheres sheeting

Laminated veneer lumber joints made with metal plate connectors were protected with wood carbon phenolic spheres (CPS) sheeting and tested for creep under fire. The effects of the carbonizing temperature of charcoal, used as raw material for the CPS sheets, the thickness, and the location of the sheet on the joint regarding the fire-resistance performance of the joint were studied. The time to rupture of the joints covered with CPS sheets made from charcoal carbonized at 800°C (CPS800) was slightly prolonged compared with that of uncovered joints. On the other hand, the time to rupture of CPS sheets made from charcoal carbonized at 1600°C (CPS1600) was markedly extended. The changes in the charcoal properties due to increasing the carbonizing temperature might be the main reason the CPS1600 sheets had higher fire-resistance performance. The thickness and location of CPS1600 sheets have significant effects on the fire resistance of the joint. A highly fire-resistant laminated veneer lumber joint was obtained using a CPS1600 sheet. The CPS1600 sheet with a thickness of 3mm covering three sides of the joint prolonged the time to rupture 16-fold compared with that of unprotected joints.Part of this paper was presented at the 4th International Wood Science Symposium, Serpong, Indonesia, September 2002     

Detection of DNA closely related to 'Candidatus Rickettsia principis' in Haemaphysalis danieli recovered from cattle in Xinjiang Uygur Autonomous Region Area, China

Tick DNA samples from cattle in Xinjiang Uygur Autonomous Region Area, China, were examined for Rickettsia infection by citrate synthase gene-based PCR and sequencing. Four positive samples were detected from Haemaphysalis danieli and high levels of similarity were found with recently detected 'Candidatus Rickettsia principis.'