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181.
Takanori Ohsako Takafumi Ohgushi Hino Motosugi Kouzou Oka 《Genetic Resources and Crop Evolution》2008,55(7):1047-1053
Genetic variability within and among eight landrace populations of tea (Camellia sinensis (L.) O. Kuntze) located in southern Kyoto, Japan, was surveyed with six microsatellite markers. The average number of alleles
per locus was 3.83 to 4.67 for landrace populations, whereas the corresponding value among modern cultivars and breeding lines
was 6.63. Expected heterozygosity values averaged over loci within landrace populations ranged from 0.498 to 0.723. A similar
level of variation, 0.682, was observed for cultivars and breeding lines. High fixation index values (0.177–0.417) for each
population are consistent with biparental inbreeding within the population. Genetic differentiation among local populations
was extremely low with F
ST = 0.062, although AMOVA revealed significant differentiation among landrace populations. We propose that these populations
share a common ancestral gene pool and that some degree of artificial selection within each population has been performed
by local farmers. Neighbor-joining analysis revealed that genetic relationships among populations reflect geographical location
of populations. This might result from more frequent genetic exchange by nearby farmers. 相似文献
182.
Antioxidant and antiulcerative properties of phenolics from Chinese quince, quince, and apple fruits
Hamauzu Y Inno T Kume C Irie M Hiramatsu K 《Journal of agricultural and food chemistry》2006,54(3):765-772
To evaluate the health benefits of Chinese quince and quince phenolics, their antioxidant properties and antiulcerative activity were investigated in comparison with apple phenolics as a reference. The strength of antioxidant activity and DPPH radical scavenging activity of these fruit phenolics varied according to different in vitro evaluation systems, whereas the antioxidative property of rat blood increased in all rats orally administered phenolics. Ferulic acid and isoferulic acid were detected as major metabolites in rats given apple phenolics, quince phenolics, and 5-caffeoylquinic acid standard. (-)-Epicatechin and its 3'-O-methyl ether could be detected in rats administered apple phenolics and (-)-epicatechin standard. In the ethanol-induced gastric ulcer, pre-administration of Chinese quince and quince phenolics suppressed the occurrence of gastric lesions in rats, whereas apple phenolics seemed to promote ulceration. The trend of myeloperoxidase activity was similar to that of the ulcer index. The results showed that Chinese quince and quince phenolics might have health benefits by acting both in blood vessels and on the gastrointestinal tract. 相似文献
183.
184.
Naomi TERAMOTO Masanari IKEDA Hidetoshi SUGIHARA Takanori SHIGA Takashi MATSUWAKI Masugi NISHIHARA Kazuyuki UCHIDA Keitaro YAMANOUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(9):1416
Rhabdomyosarcoma (RMS) is an aggressive type of soft tissue sarcoma, and pleomorphic RMS is a rare subtype of RMS found in adult. p16 is a tumor suppressor which inhibits cell cycle. In human RMS, p16 gene is frequently deleted, but p16-null mice do not develop RMS. We reported that genetic ablation of p16 by the crossbreeding of p16 knock-out rats (p16-KO rats) improved the dystrophic phenotype of a rat model of Duchenne muscular dystrophy (Dmd-KO rats). However, p16/Dmd double knock-out rats (dKO rats) unexpectedly developed sarcoma. In the present study, we raised p16-KO, Dmd-KO, and dKO rats until 11 months of age. Twelve out of 22 dKO rats developed pleomorphic RMS after 9 months of age, while none of p16-KO rats and Dmd-KO rats developed tumor. The neoplasms were connected to skeletal muscle tissue with indistinct borders and characterized by diffuse proliferation of pleomorphic cells which had eosinophilic cytoplasm and atypical nuclei with anisokaryosis. For almost all cases, the tumor cells immunohistochemically expressed myogenic markers including desmin, MyoD, and myogenin. The single cell cloning from tumor primary cells gained 20 individual Pax7-negative MyoD-positive RMS cell clones. Our results demonstrated that double knock-out of p16 and dystrophin in rats leads to the development of pleomorphic RMS, providing an animal model that may be useful to study the developmental mechanism of pleomorphic RMS. 相似文献