用重组核衣壳蛋白ELISA检测PRRSV抗体的研究

ＰＲＲＳＶ重组Ｎ蛋白ＥＬＩＳＡ检测人工感染猪血清７５份,与ＩＤＥＸＸ公司ＥＬＩＳＡ试剂盒及ＩＦＡ的符合率分别为９７．３％和１００％。检测疫苗免疫猪血清,阳性率１００％。在免疫后６ｄ就能检出抗体,比ＩＦＡ敏感。检测８５份我国田间猪血清,阳性率１８．８％。     

湘西黄牛肉质特性研究

为研究我国南方优良地方品种湘西黄牛的肉质特性,选用8头湘西黄牛青年公牛,对肉质中的常规营养成分、氨基酸和脂肪酸进行了测定.结果表明:鲜肉中脂肪含量为4.80％,蛋白质含量为23.21％;饱和脂肪酸(SFA)含量为48.43％,不饱和脂肪酸含量(UFA)为41.00％,不饱和脂肪酸在总脂肪酸(TFA)的比例为45.85％...     

畜禽养殖场环境微生物致病性和耐药性调查研究

为了解掌握养殖场中环境微生物对养殖业和公共卫生造成的影响,初步对我国养殖场环境微生物进行了研究。对吉林、湖北等省份的部分养猪场和养鸡场环境采样,并进行大肠杆菌和沙门氏菌分离鉴定,同时对从鸡舍和猪舍内分离大肠杆菌和沙门氏菌进行动物致病性实验及对13种抗生素的耐药性实验。实验结果表明,所有实验的沙门氏菌和90.7%的大肠杆菌菌株对小白鼠有致病性;所有分离菌株对磺胺类药物的耐药性非常严重,其次是对四环素,对其他药物也有不同程度的耐药性。     

熊蜂滞育及调控机制研究进展

滞育是绝大多数昆虫为了在不利于自身生存的极端自然环境中存活下来而进行的一种生理过程,是昆虫与环境长期协同进化的结果。熊蜂滞育过程属于成虫生殖滞育,其滞育进程可能受保幼激素水平、热激蛋白表达以及物质代谢等方面的影响,但其具体的滞育调节机制尚未清楚。本文从昆虫滞育的相关假说、熊蜂滞育进程以及熊蜂滞育机制调控进展等方面进行阐述,以期为后续熊蜂工厂化繁育中滞育技术的突破奠定基础。     

不同激素剂量对幼鼠超数排卵及超排卵质量的影响

应用国产激素制剂对３～８周龄昆明种小白鼠进行了超数排卵处理,重点观察了不同激素剂量对幼龄小鼠的超排效果及超排卵质量的影响。实验结果表明,以ＰＭＳＧ与ｈＣＧ各１０ＩＵ剂量超排３～８周龄小鼠,５周龄小鼠的超排效果（平均获卵８３．８±１０．２个）极显著（Ｐ＜０．０１）好于４～８周龄小鼠的超排效果（平均获卵３９．２～４９．８个）,而３周龄小鼠超排卵受精后体外培养的卵裂率（９７．１％）、桑椹胚率（８６．８％）和囊胚率（６５．１％）与４～８周龄超排卵的（分别为８５．５％～１００．０％、９４．９％～１００．０％和６３．２％～８７．７％）相近;以ＰＭＳＧ与ｈＣＧ各５ＩＵ剂量超排３～５周龄小鼠,３周龄小鼠的超排效果（平均获卵５９．０±６．５个）显著（Ｐ＜０．０５）好于４周龄小鼠的超排效果（平均获卵３１．４±４．９个）,并极显著（Ｐ＜０．０１）好于５周龄小鼠的超排效果（平均获卵２１．４±３．３个）;以ＰＭＳＧ与ｈＣＧ各１５ＩＵ剂量超排３～５周龄小鼠,３周龄小鼠的超排效果（平均获卵４８．６±７．３个）显著（Ｐ＜０．０５）好于５周龄小鼠的超排效果（平均获卵２５．２±５．８个）,也好于４周龄小鼠的超排效果（平均获卵４０．２±５．１个     

不同贮藏条件鸡蛋的脂类过氧化作用

本文以ＴＢＡ光度法分析了不同贮藏条件下鸡蛋脂类的过氧化作用,并分析了温度、氧对过氧化作用的影响。结果表明,在贮藏条件下,鸡蛋蛋黄中伴有明显的脂类过氧化过程。脂类过氧化物（ＬＰＯ）的水平随贮藏时间的延长而不断增加（Ｐ＜０．０１）,但蛋清中的ＬＰＯ在贮藏过程中没有明显的变化。低温和缺氧可明显抑制鸡蛋脂类的过氧化作用（Ｐ＜０．０１）。     

The effects of road transportation on physiological responses and meat quality in sheep differing in age

The aim of this study was to determine the effects of 8 h of road transportation on physiological responses and meat quality traits of sheep at 6, 12, and 24 mo of age. Seventy-two male sheep were equally divided into transported (TRANS) and nontransported (CON) treatments (n = 36), and each treatment was subdivided into 3 groups by age (n = 12). Sheep in CON groups were weighed, blood sampled, and slaughtered, whereas sheep in TRANS groups were weighed, transported, blood sampled, and slaughtered to collect meat samples. The BW of sheep in TRANS groups was reduced significantly (P < 0.001) compared with CON sheep, and older sheep lost more BW than younger animals. However, dressing percentages of TRANS sheep were significantly (P < 0.001) greater than those of CON sheep. Some meat quality variables were affected by transportation, and responses of different ages of sheep varied. Total pigment content and lipid oxidation of LM and gluteus medius of TRANS sheep increased significantly (P < 0.001) compared with CON sheep. Cooking loss of LM and gluteus medius was influenced significantly (P < 0.01) by interaction effect between transportation and age, and the values for 6-mo-old TRANS sheep were less than those of CON sheep. Serum total protein (P = 0.036), globulin (P = 0.026), triglyceride (P < 0.001), and total cholesterol concentrations (P = 0.028) of TRANS sheep decreased compared with CON sheep. Serum NEFA concentration of TRANS sheep increased in relation to CON sheep with a significant interaction (P < 0.001) between transportation and age effect. Numbers of white blood cells were influenced (P = 0.002) by an interaction effect between transportation and age, and values for 6-mo-old sheep were not influenced by transportation. Numbers of platelets were influenced (P = 0.014) by age; they decreased more in 6-mo-old sheep than in older sheep. Transportation and age had no significant effects (P > 0.05) on packed cell volume and lymphocyte number. Serum creatine kinase activities in TRANS sheep were affected (P = 0.01) by an interaction effect between transportation and age. The 2-thiobarbituric acid reactive substances in serum of TRANS sheep increased (P = 0.009) with the greatest change for 6-mo-old compared with older sheep. In conclusion, 8 h of road transportation resulted in greater heme pigment concentrations but improved meat tenderness and induced physiological responses of sheep. However, different ages of sheep showed different responses to the present transportation pattern.     

浅析新疆运输动物及动物产品的检疫监督

<正>运输动物及动物产品的检疫监督管理是指:动物卫生监督机构依照《中华人民共和国动物防疫法》规定,在动物以及动物产品运输过程的动物检疫实施监督管理。一般在动物及其动物产品运载频繁的车站、码头、机场、国道等交通要道上设立检疫监督检查站,负责动物检疫监督检查工作。对过往运输动物及动物产品的车辆要查证验物,对在运输途中发生传染病的牲畜及其尸体,要按规定进行处理,对装运病畜的车辆、船只、飞     

不同日粮对泌乳奶牛干物质采食量及消化率的影响

本试验旨在研究在不同日粮条件下对奶牛干物质采食量(DMI)及养分消化率的影响。用4头安装永久瘤胃瘘管和十二指肠瘘管的泌乳奶牛(体重为483±21kg,泌乳日龄为175±6天)通过4×4拉丁方试验设计,日粮包括30:70CW组(粗料仅由羊草组成)、30:70CCA组(粗料为玉米青贮+苜蓿+羊草)、50:50CCA组和65:35CCA组,共设4期试验,每期23天。30:70CCA组干物质采食量与有机物采食量极显著低于其它日粮组(P<0.01),30:70CCA组能量采食量显著低于其它日粮组,30:70CW组DM、OM在瘤胃消化率(P<0.05)及全肠道消化率(P<0.01)均显著低于其它日粮组;NDF、ADF瘤胃及全肠道消化率均无显著差异。65:35CCA组产奶量极显著高于其它组(P<0.01)。