不同氮素形态营养及水分胁迫对分蘖期水稻水分吸收及光合特性的影响

室内营养液培养,采用聚乙二醇(PEG6000)模拟水分胁迫处理、根系烫伤处理测定质外体途径水分吸收和0.5mmol.L-1HgC l2处理抑制水通道蛋白活性的方法,在3种供氮形态下(NH4+-N、NO3--N、NH4+-N与NO3--N(等浓度混合)),研究了苗期水稻的生长和水分吸收状况。结果表明,无论在非水分胁迫条件下,还是在水分胁迫条件下,NH4+-N与NO3--N混合处理的水稻生物量最大。在非水分胁迫条件下,NO3--N处理和NH4+-N与NO3--N混合处理的水稻单位干质量吸水量高于NH4+-N处理的水稻;烫伤处理和加HgC l2抑制水通道蛋白活性后,各处理水稻在单位时间内单位干质量水分吸收量都下降。NO3--N与NH4+-N混合营养处理的水稻净光合速率、气孔导度和蒸腾速率高于NH4+-N处理和NO3--N处理。在根系烫伤处理和HgC l2处理后0.5 h内,各处理水稻的净光合速率、气孔导度和蒸腾速率迅速下降。     

基于虚拟水的福建省2006年水足迹评价

本文用虚拟水水足迹计算方法计算分析了2006年福建省的水足迹,采用相关评价指标对水足迹现状进行分析评价.结果表明,福建省2006年的水足迹总量为363.42亿m3,其中,内部水足迹为312.55亿m3,外部水足迹为50.878亿m3,人均水足迹为1021.4 m3,水足迹占可利用水资源的22.39%,水资源消费自给率为86%.针对福建省水足迹状况、结构组成、虚拟水进出口贸易等方面对水资源综合管理和开发利用提出了几点建议.     

Identification of Suspected Stem/progenitor Cells in Bovine Mammary Epithelial Cells Culture System

To develop the potential function of dairy cow mammary stem cells (DCMECs) in regulation of lactation,we identify putative DCMECs which were BrdU label retaining epithelial cells,at the same time,analysis the location of two new mammary stem cells molecular marks FNDC3B and PROCR to verify the feasibility of them to indicate DCMECs.The mRNA levels of prolactin,growth hormone,insulin-like growth factor-1 and their receptors were detected along with cell passage by Real-time quantitative PCR.The results showed that the proportion of BrdU label-retaining epithelial cells was nearly 0.4% after 25 d continuous culture (passaged 4 times) and few cells were positive for FNDC3B or PROCR.Moreover,we observed the BrdU labelled epithelial cells by asymmetric division.The mRNA levels of prolactin,growth hormone,insulin-like growth factor-Ⅰ and their receptors in primary and passage cells were extremely significant difference(P<0.01).DCMECs would rapidly lose some physiological characteristics and the ability of milk synthesis when not under the condition of induction of lactation differentiation,but a certain percentage of mammary stem/progenitor cells will be retained,whose potential effects on the regulation of lactation and mammary acinar remodeling were worthy of attention.     

马王堆医书的生态思想及当代价值研究

生态与健康都是当今社会关注的焦点。马王堆医书作为重要的医学文献,蕴含着丰富的生态思想。医书不仅对人的生存状态进行了阐释,还饱含了人类需要主动认识自然、了解自然、尊重自然界一切其他生物,最终达到人与自然、人与人、人与社会相和谐的生态理念。研究医书的生态思想对于我们形成中医养生理念、促进社会和谐发展和推进生态文明建设具有重要价值;同时,还能够为生态医学的理论架构提供基础认识。     

玉米单交种不同收获时问对其种子萌发过程中保护酶活性的影响

以玉米单交种郑单958为试验材料,研究了不同收获时间对种子发芽率,以及种子萌发过程中保护酶活性和丙二醛含量的影响.结果表明,授粉后40d收获的玉米种子其发芽率最低,未达到国家玉米良种发芽率85%的标准;授粉后45～60d收获的玉米种子其发芽率为89.7%～97.3%.均高于国家标准,其中,55d收获的玉米种子其发芽率最高.随着玉米收获期的推迟,玉米种子在萌发过程中,SOD、CAT和POD活性均呈上升趋势,而MDA含量呈下降变化.授粉后50d、55d和60d收获的种子萌发过程中SOD、CAT和POD活性都显著高于40d、45d收获的单交种,而其MDA含量都显著低于40d、45d收获的单交种.研究表明玉米单交种的最佳收获时间为授粉后55d.     

滇中岩溶高原滇青冈原生林植物水分利用来源的稳定同位素分析

为阐明滇中岩溶高原滇青冈Cyclobalanopsis glaucoides原生林植物水分利用机理及其对岩溶干旱生境的适应策略,运用氧稳定同位素技术,分析了滇青冈,云南木樨揽Olea yue nnanensis,清香木Pistacia weinmannifolia等3种不同年龄阶段常见优势木琴植物小枝木质部水分及土壤水、表层岩溶带水氧同位素值的相关性,确定植物水分来源,并通过IsoSource模型计算不同潜在水源对滇青冈原生林的贡献。结果表明：滇青冈及其伴生的乔木水分利用层位及利用比例呈现时空差异,并形成明显的水分竞争关系。旱季,滇青冈、成熟云南木樨揽、幼龄清香木主要利用55～115 cm深层土壤水,成熟的滇青冈和清香木对表层岩溶带水利用比例增加。雨季,成熟的滇青冈、云南木樨揽、清香木和幼龄清香木因大气降水对浅表层土壤的补给主要利用20-55cm土壤水,利用比例分别为100％,33．3％,66．0％,37．4％,幼龄植株对表层岩溶带水的利用比例大于成熟植株。图2参23     

河套灌区畦田内不同位置土壤入渗特性及影响因素分析

为明确河套灌区畦田田块不同位置土壤的入渗特性,通过野外试验和室内检测对试验区田块不同位置土壤理化性质和入渗过程进行分析,并基于土壤入渗模型对试验区田块不同位置的土壤入渗过程进行拟合,同时探讨影响土壤入渗过程的影响因素.研究结果表明:试验区田块内不同位置土壤理化性质及入渗特性具有一定差异性且不同位置土壤入渗特性与土壤理化...     

补光对兰云杉和红皮云杉生长的影响

云杉苗期生长缓慢是生产中的主要问题,补光可促进苗木生长,缩短育苗周期,提高育苗效率.研究不同光源(阳光灯、镝灯、碘钨灯)、不同光照时间对兰云杉和红皮云杉1年生苗苗高、地径、侧枝数、根系、生物量等生长性状的影响,结果表明:光照处理可以抑制苗木休眠,促进其持续生长,对2种云杉的各生长指标均有显著的促进作用;不同光源、不同光...     

Intestinal Microbiota in Large Yellow Croaker,Larimichthys crocea,at Different Ages

The polymerase chain reaction–denaturing gradient gel electrophoresis (PCR‐DGGE) of 16S ribosomal RNA gene was used to investigate bacterial communities in the intestines of large yellow croaker at six different ages (12 d, 18 d, 26 d, 40 d, 3 mo, and 1 yr old) as well as within the corresponding feed and culture water. In addition, Illumina Miseq sequencing was utilized to compare intestinal microbiota between 12‐d‐old and 1‐yr‐old individuals. PCR‐DGGE results revealed that the culture water had the highest bacterial diversity, followed by the feed, while the intestines had the lowest diversity. The intestinal microbiota at six ages changed severely; however, the change did not follow any trend. The large yellow croaker intestines harbored specific bacterial communities that differed from those in both feed and water. Illumina Miseq sequencing results revealed that the diversity of intestinal bacteria in 12‐d‐old fish was higher than that in 1‐yr‐old fish, and the bacterial composition differed significantly between them. γ‐Proteobacteria and Pseudoalteromonas supplied the most abundant phylum and genus in the 12‐d‐old fish intestine. However, in the 1‐yr‐old fish intestine, Firmicutes and Clostridium were the most dominant, respectively. The study may contribute to a better understanding of gut microbiota and dynamics of the large yellow croaker and the relationship with their surrounding environment.     

闽江河口稻田土壤甲烷产生潜力及其对炉渣添加的响应

对闽江河口稻田甲烷产生潜力及其对炉渣添加的响应特征进行了初步研究,结果表明,(1)稻田土壤甲烷产生潜力在培养第3天出现峰值,10-20 cm土壤是甲烷产生的主要发生层;(2)甲烷产生潜力的剖面变异性低于时间变异性;(3)炉渣添加对0-40 cm土壤层次甲烷产生潜力具有抑制作用,但不同土壤层次甲烷产生潜力对炉渣添加比例的响应有所不同。