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Techniques for in vitro production of embryos have been developed world-wide in different species, with promising results in human and ruminants. Thousands of human IVF-babies have been born during the last 20 years and thousands of in vitro -produced calves have been born since the late 1980s. With current methods for bovine in vitro fertilization, about 30–40% of in vitro -fertilized bovine oocytes develop further to the blastocyst stage and can be used for transfer. A proper evaluation of blastocyst quality remains however, an important challenge for every researcher involved in embryology and for every clinician who wants to select the best embryos for transfer. This review attempts to summarize the different methods available for estimation of blastocyst quality with a special emphasis upon differential staining. 相似文献
43.
高等级公路生物护坡工程模式设计 总被引:11,自引:0,他引:11
根据高等级公路边坡的土体特性 ,将边坡分成硬质材料覆盖坡面、松软基质坡面、紧实基质坡面三种类型 ,按照不同类型边坡的土壤组成结构、坡角大小及物种选择的理论依据和生物护坡工程实践 ,提出了各类型的边坡应采取的绿化施工手段及物种选择与组合搭配的一般规律 相似文献
44.
苜蓿施用污泥效果的研究Ⅰ对苜蓿生长及元素含量的影响 总被引:5,自引:2,他引:3
将兰州市七里河污水处理厂生活污泥施用于人工种植的新疆大叶苜蓿草地,探讨了不同施用量对苜蓿生长及各元素含量的影响。研究结果表明,施用1.0-2.0kg/m^2污泥,苜蓿地上生物量显著增加,第一年增产23%-31%,第二年增产15%-31%。施用污泥苜蓿地上和地下部组织中N,P,Ca,Mg和Mn的含量总体呈增加趋势,Fe含量显著增加,而K和重金属元素Ni、Pb、As和Hg的含量与对照无显著差异,Zn和Cu的积累增多,但地上部组织中重金属元素均未超过植物中的平均含量,施用污泥未对新疆大叶苜蓿造成重金属污染。 相似文献
45.
喹诺酮类药物研究进展及在兽医临床的应用问题 总被引:12,自引:0,他引:12
喹诺酮类药物已成为合成抗菌芗中最具有开发前途的一类药物。兽医临床常用药物有不同的特点和应用。与此同时,此类药物的毒性反应、耐药性及药物残留等问题不断出现,针对各方面的问题,提出此类药物的发展前景。 相似文献
46.
Between September 1982 and March 1984, 101 Western Australian piggeries with 15 or more sows were surveyed to determine the prevalence of internal parasites and examine the relationship between parasitism and management practices. Faecal samples were collected from 20 pigs in 4 age groups in randomly selected piggeries, and examined for the presence of eggs of helminth parasites and protozoan cysts. Evidence of nematode parasites was found in 79% of piggeries. Sows were more commonly affected than other classes of pigs with worm eggs being found in 68% of herds. Oesophagostomum spp was the most prevalent worm species, being found in pigs from 65% of piggeries and in sows in 60% of herds. Ascaris suum was the most common species of worm found in growing pigs. There was no evidence of infection with either Metastrongylus spp or Strongyloides spp in any of the herds sampled. Oocysts of coccidia were found in pigs from 56% of piggeries and Balantidium coli cysts were detected in pigs from 42% of piggeries sampled. 相似文献
47.
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49.
C. P. Verschueren P. J. Selman J. J. M. de Vijlder J. A. Mol 《Domestic animal endocrinology》1991,8(4):509-519
Canine thyroglobulin (cTg) has been isolated and purified. It has similar electrophoretic patterns as Tg from other mammalian species. The main fraction had a MW of 660,000, whereas also fractions of a MW of approximately 1,300,000 (dimer) and 330,000 (subunit) were present. The iodine content was 0.8 to 1.0 % (w/w). cTg did not cross-react with antibodies against human Tg to a degree that would allow the use of a radioimmunoassay for human Tg for the determination of cTg in serum or plasma. Therefore a polyclonal antiserum was raised against cTg and a homologous radioimmunoassay was developed, which was sensitive (0.4 μg/l) and specific (cross-reactivity in cTg assay of human Tg, goat Tg, T4, T3, and DIT < 0.01 %).
Plasma Tg levels in normal dogs of both sexes and aged 3–15 years amounted to 192 ± 73 μg/l (mean ± SD, n=30). There was no relation between plasma Tg and T4 levels. 相似文献
50.
J.A. Díez P. Hernaiz M.J. Muñoz A. de la Torre A. Vallejo 《Soil Use and Management》2004,20(4):444-450
Abstract. The repeated application of pig slurry to agricultural soils may result in an accumulation of salts and a risk of aquifer pollution due to nitrate leaching and salinization. Under Mediterranean conditions, a field experiment on a sandy loam soil (Typic Xerofluvent) was performed with maize (Zea mays) in 1998, 1999 and 2001 to study the effects of applying optimal (P1) and excessive rates (P3) of pig slurry on soil salinization, nitrate leaching and groundwater pollution. The rate of pig slurry was established considering the optimal N rate for maize in this soil (170, 162 and 176 kg N ha?1 for 1998, 1999 and 2001, respectively). Pig slurry treatments were compared to an optimal N rate supplied as urea (U) and a control treatment without N fertilizer (P0). The composition of the slurries showed great variability between years. Mean NO3? leaching losses from 1998 to 2001 were 329, 215, 173 and 78 kg N ha?1 for P3, P1, U and P0 treatments, respectively. The amount of total dissolved salts (TDS) added to the soil in slurry application between 1998 and 2001 was 2019 kg TDS ha?1 for the P1 treatment and 6058 kg TDS ha?1 for the P3 treatment. As a consequence, the electrical conductivity (EC) of the slurry‐treated soils was greater than that of the control soil. The EC correlated significantly with the sodium concentration of the soil solution. Over the entire experimental period, 2653, 2202 and 2110 kg Na ha?1 entered the aquifer from the P3, P1 and P0 treatments, respectively. The P3 treatment did not significantly increase grain production in 1999 and 2001 compared with that achieved with the optimal N rate treatment (P1). This behaviour shows the importance of establishing application guidelines for pig slurry that will reduce the risk of soil and groundwater pollution. 相似文献