热应激蛋白70与细胞凋亡

热应激蛋白 (heatstressprotein,HSP)或热休克蛋白 (heatshockpro tein,HSP)是机体受到应激原的刺激后产生的几族蛋白质 ,具有高度保守性 ,对维持细胞生存和内环境稳定起重要作用 [1]。多种应激原如高热、重金属、饥饿、缺氧、缺血等都可诱导HSP的表达 ,但人们习惯上仍称其为热应激蛋白或热休克蛋白 ,有时也称为应激蛋白 (stressprotein,SP)。一般来说 ,根据HSP的同源性、功能和分子量 ,主要HSP可分成4个家族 :小分子量HSP家族、HSP70家族、HSP90家族和大分子量HSP家族。HSP70家族是HSP中最保守和最重要的一族 ,在大多数生物…     

伪狂犬病病毒野毒荧光定量PCR检测方法的建立

根据伪狂犬病病毒gE基因的序列,设计和合成了一对特异的可用于检测伪狂犬病病毒野毒的PCR引物和一条Taqman荧光探针,采用Li ght Cycl e 480荧光定量PCR仪,建立了一种可实时定量检测伪狂犬病病毒野毒的荧光定量PCR技术。该方法的线性范围为1.0×102～1.0×1010拷贝,灵敏度可达4拷贝。检测速度快,仪器的运行时间仅为1 h。对13株猪伪狂犬病病毒野毒进行了检测,结果均为阳性;与伪狂犬病gE基因缺失疫苗、猪细小病毒和鸭瘟病毒无非特异性反应。与病毒分离培养比较,该方法具有快速、灵敏、特异、定量、重复性好等优点,可望用于临床上伪狂犬病病毒野毒与疫苗毒的区分,伪狂犬病病毒野毒的检测和病毒分布的研究等。     

Canine renal failure syndrome in three dogs

Three dead dogs were brought to the College of Veterinary Medicine, Kyungpook National University for study. Clinically, all the dogs showed emaciation, anorexia, depression, hemorrhagic vomiting and diarrhea for 7~10 days before death. All the clinical signs were first noted for about one month after feeding the dogs with commercial diets. At necropsy, all 3 dogs had severe renal damage with the same green-yellowish colored nephroliths in the renal pelvis. They also showed systemic hemorrhage and calcification of several organs, which might have been induced by uremia. Microscopically, necrosis, calcification and calculi were detected in the renal tubules, and especially in the proximal convoluted tubules and collecting ducts of the kidney. These findings were supportive of a mycotoxic effect, and especially on their kidneys. However, the precise cause of the toxic effect in these cases of canine renal failure could not be determined.     

以超滤技术浓缩猪细小病毒含毒细胞液的试验

在研制猪细小病毒灭活疫苗时,应用了超滤浓缩技术,提高了合毒细胞培养液中的抗原含量。为了保证浓缩的流量,减少对滤膜的污染,对含毒细胞培养液作了预处理一离心及微滤,以便除去细胞培养液中的细胞碎片、蛋白质等大分子物质及固形微粒。超滤时选用50000Da滤膜,在20psig压力下,经过5倍浓缩的PPV细胞培养液,血凝滴度提高2个滴度以上。病毒含量测定表明,TCID50／0．2mL由107左右升至109以上,以其配制疫苗,能显著地提高疫苗的免疫原性。超滤技术具有易于操作、高效、分离精度高、没有二次污染等优点,可以根据需要选择不同的浓缩浓度,对保证疫苗的质量具有重要作用。     

犬常见寄生虫病及防治

犬寄生虫病是犬病临床的常见病之一。犬患寄生虫病的种类很多,如果不能及时发现犬患上了寄生虫病或者未能及时准确地给予治疗,采取相应的防治措施,将会严重损害犬的健康。有些犬患寄生虫病还可以传染给人类,形成人犬共患危害人类身体健康。犬常见的寄生虫病有:蛔虫病、绦虫病、犬血丝虫病、     

猪床垫料与替抗的诡辩

1概念解析随着我国畜牧业的飞速发展,畜禽粪便成了环境的最大污染源,对环境的污染愈发严重。为控制粪便污物的排放,创造良好的生态养殖条件,国家大力推广发酵床养殖技术,发酵床主要是由有机垫料组成,垫料原料通常包括秸秆、锯末、木屑等,将其平铺与猪舍内,猪养于其上,排泄物排至垫料上,经微生物发酵,可实现粪污的低排放、低污染[1-2]。     

新城疫La Sota、V4疫苗免疫鸡和免疫攻毒鸡的特异性IgA抗体动态变化规律比较

应用间接ELISA对新城疫LaSota、V4疫苗免疫鸡及免疫攻毒鸡的IgA抗体动态变化进行了测定和比较。试验表明，LaSota和V4免疫鸡泪液中特异性IgA均在免疫后第5天出现，8天开始明显升高，与血清中IgG出现时间相似，免疫后21天达到高峰。LaSota免疫鸡的泪液IgA抗体水平略高于V4免疫鸡；而两免疫组哈德氏腺(HG)中的特异性IgA高峰出现较迟。免疫攻毒鸡泪液中的特异性IgA抗体水平均先呈短暂的升高，之后下降的趋势，而HG中的IgA则首先表现降低，之后很快升高，5天后趋于下降，两攻毒组差异不显著，对IgA回忆应答均不明显。     

棕尾虹雉短角鸟虱新种描述（食毛目：短角鸟虱科,短角鸟虱属）

本文报道了从上海动物园棕尾虹雉（Lophophorusimpejanus）体上所采集的鸟虱,经鉴定后,该虫神为头角亚目（Amblycera）短角鸟虱科（Menoponidae）短角鸟虱属（Menopon）一新种,文内对其所具有的分类特征进行了描述,并绘图。     

Black yeast-like fungi associated with Lethargic Crab Disease (LCD) in the mangrove-land crab, Ucides cordatus (Ocypodidae)

Lethargic Crab Disease (LCD) caused extensive epizootic mortality of the mangrove land crab Ucides cordatus (Brachyura: Ocypodidae) along the Brazilian coast, mainly in the Northeastern region. The disease was named after the symptoms of slow movement of infected crabs. Causative agents were suspected to be two black yeast-like fungi of the family Herpotrichiellaceae (ascomycete order Chaetothyriales), judged by infected tissue biopsies from moribund U. cordatus. The aim of the present study is to prove that two species are involved in the disease: the recently described black yeast Exophiala cancerae, but also a less virulent, hitherto undescribed fonsecaea-like species, introduced here as the novel species Fonsecaea brasiliensis. Strains were identified by ITS rDNA sequencing, and species borderlines were established by multilocus sequencing and AFLP analysis. Fonsecaea brasiliensis proved to be closely related to the pathogenic species Cladophialophora devriesii which originally was isolated from a systemic infection in a human patient. The virulence of F. brasiliensis is lower than that of E. cancerae, as established by artificial inoculation of mangrove crabs.     

澳大利亚墨尔本WHO流感中心对南极洲企鹅的禽流感监测

2013年,澳大利亚墨尔本世界卫生组织流感参考和研究合作中心对南极企鹅开展了禽流感病毒监测。在270份企鹅血样中,检出34份禽流感抗体阳性,抗体阳性率为16%;对301份咽喉?泄殖腔拭子进行禽流感病原学检测,检出8份阳性,病原阳性率为2.5%,并成功分离到4株H11N2禽流感病毒。企鹅携带禽流感病毒这一事实,引发了全球共同抗击全球化动物疫情的思考。