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51.
本研究以p BI121为植物表达载体,在已构建的无融合生殖基因表达载体p BI121-Mh SERK1和p BI121-Mhd SERK1的基础上,利用根癌农杆菌介导法,将苹果属无融合生殖相关基因SERK1转入受体植株烟草中。研究结果表明:烟草叶片浸染后,经共培养和选择培养形成再生芽,分别获得7株p BI121-Mh SERK1和6株p BI121-Mhd SERK1转烟草抗性植株,PCR检测证明已成功获得Mh SERK1和Mhd SERK1烟草转化株系。本研究可为下一步无融合生殖相关基因功能验证研究奠定基础。 相似文献
52.
Jens Frederik Agger Suman Paul Anna-Bodil Christoffersen J?rgen Steen Agerholm 《Acta veterinaria Scandinavica》2013,55(1):80
The aim was to identify risk factors associated with Coxiella burnetii antibody positivity in bulk tank milk (BTM) samples from 100 randomly selected Danish dairy cattle herds. Antibody levels were measured by an enzyme-linked immuno-sorbent assay. Before testing the herds, the farm managers were interviewed about hired labour, biosecurity, housing and herd health during the 12 months prior to the study. Variables considered important for C. burnetii antibody positivity in multivariable logistic regression analysis included the sharing of machines between farms (OR = 3.6), human contacts (OR = 4.2), artificial insemination by other people than artificial insemination technicians (OR = 7.7), routine herd health contract with the veterinarian (OR = 4.3) and hygiene precautions taken by veterinarians (OR = 5). In addition, herd size, hired labour, trading of cattle between farms, quarantine and use of calving and disease pens also showed significant association in univariable analysis. This study demonstrates that strict biosecurity is important for the prevention of infections with C. burnetii. 相似文献
53.
Extracts of Lantana camara var. aculeata leaves were studied for their phytochemical constituents and termiticidal effects against adult termite workers. The 5% chloroform extract was found to be significantly effective against termite workers. 相似文献
54.
Singh P Arya N Tiwari P Suman A Rai RK Shrivastava AK Solomon S 《Journal of agricultural and food chemistry》2008,56(16):7176-7183
Sugar cane is sensitive to enormous sucrose losses induced by physio-chemical and microbial changes, the severity being increased during the time lag between harvest and crushing in the mills. Minimization of the sucrose losses in the field is essential for better sugar recovery and prevention of sucrose losses. An experiment was conducted to evaluate the efficacy of glutaraldehyde and benzalkonium chloride for their effects on the microbial counts and physio-chemical changes responsible for sucrose losses. Glutaraldehyde and benzalkonium chloride (1000 + 250 ppm) reduced the losses in sucrose content to 7.1% as compared to the 30.8% loss in the control, thus improving the performance by 76.9%. The application of chemicals reduced the acid invertase activity (by 60%), lowered weight loss, titrable acidity, reducing sugars content, dextran, ethanol, and ethylene production and respiration rates. The application led to the reduction in the total bacterial, fungal, Leuconostoc, and yeast counts by 67.92, 51.3%, 26.08, and 51.2%, respectively. 相似文献
55.
Random amplified polymorphic DNA markers were used to study sub-structure and genetic differentiation amongst 31 populations
(seven cultivated and 24 wild populations) belonging to 14 Asiatic Vigna species. Ten pre-selected RAPD primers generated 152 polymorphic amplification products. Estimates of polymorphism indices
were higher for the wild taxa in comparison to the cultivated forms. FST values between populations ranged from 0.111 to 0.801 and Nei’s genetic diversity values between and within species varied
from 0.26 to 0.70 and 0.04 to 0.56 respectively. The high FST and FCT values indicated strong subdivision of populations and high differentiation among species. Analysis of molecular variance
was performed by grouping the populations conforming to specific species. AMOVA was also performed separately to better resolve
the differentiation of species within mungo–radiata complex. Molecular phylogenetic relationships amongst the species of radiata–mungo complex; namely, black gram (V. mungo (L.) Hepper), green gram (V. radiata (L.) Wilczek), V. radiata var. sublobata, V. radiata var. setulosa, V. mungo var. silvestris and V. hainiana, were studied through cluster analyses. Two distinct groups were recognized within the complex, with population samples of
V. hainiana forming one cluster. Further, V. hainiana appeared to be equidistant to both V. radiata and V. mungo. 相似文献
56.
Studies were conducted during 2010–2012 at University of Horticulture & Forestry, Solan, Himachal Pradesh, India. Four fertigation levels were tested with humic acid (combined fertigation) and without humic acid (sole fertigation or fertigation alone). The experiment comprising eight treatment combinations in Randomized Block design, replicated four times. Investigations revealed that combined fertigation significantly increased plant height (5.7%), total dry matter (7.7%), leaf area index (3.2%), chlorophyll content (4.7%) and fruit yield (9.6%) over sole fertigation. Higher available N and K in was recorded in surface soils whereas, better translocation of available P was noted at 10–20 cm soil layers under combined fertigation. Higher nutrient recovery and fertilizer use efficiency was also noted in combined fertigation. Fertigation along with humic acid resulted in 20 per cent fertilizer savings over fertigation alone. It is concluded that efficiency of fertigation can further be increased by using humic substances in any agricultural production system. 相似文献
57.
Garai Suman Garai Sanchita Maiti Sanjit Meena B. S. Ghosh M. K. Bhakat Champak Dutta T. K. 《Tropical animal health and production》2017,49(3):641-648
Tropical Animal Health and Production - Livestock is a one of the major sources of livelihood for most of the small and marginal farmers in India, particularly for rural households who live in... 相似文献
58.
Saini Suman Gupta V. K. Gururaj K. Singh D. D. Pawaiya R. V. S. Gangwar N. K. Mishra A. K. Dwivedi Deepak Andani Dimple Kumar Ashok Goswami T. K. 《Tropical animal health and production》2017,49(6):1253-1264
Tropical Animal Health and Production - Brucellosis is one of the leading causes of abortion in domestic animals that imposes costs on both economy and society. The disease is highly zoonotic and... 相似文献
59.
Suman Choudhary A.Kumaresan Manish Kumar Shivani Chhillar Hrudananda Malik Sudarshan Kumar Jai K.Kaushik Tirtha K.Datta Ashok K.Mohanty 《畜牧与生物技术杂志(英文版)》2018,(1)
Background: An oviduct-specific glycoprotein, OVGP1, is synthesized and secreted by non-ciliated epithelial cells of the mammalian oviduct which provides an essential milieu for reproductive functions. The present study reports the effects of recombinant buffalo OVGP1 that lacks post-translational modifications, and native Buffalo OVGP1 isolated from oviductal tissue, on frozen-thawed sperm functions and in vitro embryo development.Results: The proportion of viable sperms was greater(P 0.05) in the recombinant OVGP1-treated group compared to the native OVGP1-treated group at 2 h, 3 h, and 4 h of incubation. The proportion of motile sperms at3 h and 4 h of incubation; and membrane-intact sperms at 4 h was greater(P 0.05) in the native OVGP1-treated group compared to the control and recombinant OVGP1-treated groups. The proportion of capacitated and acrosome-reacted sperms was greater(P 0.05) in the native OVGP1-treated group compared to the recombinant OVGP1 group at 4 h. The rates of cleavage of embryos and their development to the blastocyst stage were greater(P 0.05) in the presence of either native or recombinant OVGP1 in comparison to control at 10 μg/mL concentration as compared to 5 or 20 μg/mL.Conclusions: The study suggests that both native and recombinant OVGP1 impart a positive effect on various sperm features and in vitro embryo development. However, native OVGP1 was found to have a more pronounced effect in comparison to recombinant non-glycosylated OVGP1 on various sperm functions except viability. Hence,our current findings infer that glycosylation of OVGP1 might be essential in sustaining the sperm functions but not the in vitro embryo development. 相似文献
60.
A practical in vitro method was tested for measuring differences in rapidly digested starch (RDS measured at 20 min), slowly digested starch (SDS between 20 and 120 min) and inaccessible digestible starch (IDS as carbohydrate digested after homogenising at 120 min to release inaccessible starch and digesting for a further 40 min) in wholegrain cereal samples retaining some grain structure, using in vitro pancreatic digestion after a “gastric” pepsin–HCl pre-treatment. The persistence of digestive capacity, the influence of homogenising on digestive capacity, and the benefit of adding supplementary amyloglucosidase after 120 min digestion were tested as effects on capacity to digest a further 2.5 g portion of finely ground wholegrain added to selected 120 min digests. The results showed that sufficient digestive capacity remained to digest the IDS released by homogenising the wholegrains after 120 min prior digestion. The method measured all fractions with good precision (CV < 6%), and was shown to be capable of measuring a wide degree of variation in starch fractions of differing digestibility in rolled oats from an oat breeding population, so may be useful in detecting the effects of variations in grain structure that influence the glycemic impact of wholegrain products. 相似文献