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71.
Peribiliary cysts associated with severe liver disease: a previously unrecognized tumor in a lion (Panthera leo). 总被引:1,自引:0,他引:1
Chi-Ho Yu Kyoo-Tae Kim Du-Na Hwang Ji-Young Yhee Chang-Taek Moon Tai-Young Hur Jung-Hyang Sur 《Journal of veterinary diagnostic investigation》2007,19(6):709-712
A 13-year-old male lion (Panthera leo) from Dae Jeon Zoo, Republic of Korea, presented with clinical signs of lethargy and anorexia. Despite treatment with antibiotics and fluid therapy, the lion died 6 days after initial presentation. Postmortem examination revealed multiple masses measuring 5-10 cm in diameter and cysts throughout the liver. A diagnosis of spontaneous peribiliary cysts was made on the basis of microscopic lesions as well as special staining and immunohistochemical characteristics. Histologically, the neoplasm was surrounded and composed of compact collagenous tissue. The inner cystic single layer resembled biliary mucosa and was composed of cuboidal or flattened epithelial lining that was strongly immunopositive for cytokeratin AE1/AE3. This layer was surrounded by fibrous tissue that stained blue by Masson's trichrome staining. Given the presence of multiple organized cysts in the liver, the lesion was consistent with peribiliary cysts. To the authors' knowledge, this is the first report of peribiliary cysts in an animal. 相似文献
72.
Zach Bohannan Raghavendra Sumanth Pudupakam Jamin Koo Harrison Horwitz Josephine Tsang Amanda Polley Enyang James Han Elmer Fernandez Stanley Park Deanna Swartzfager Nicholas Seah Xi Qi Chantal Tu Wendi Velando Rankin Douglas H. Thamm Hye‐Ryeon Lee Sungwon Lim 《Veterinary and comparative oncology》2021,19(1):160-171
We report a precision medicine platform that evaluates the probability of chemotherapy drug efficacy for canine lymphoma by combining ex vivo chemosensitivity and immunophenotyping assays with computational modelling. We isolated live cancer cells from fresh fine needle aspirates of affected lymph nodes and collected post‐treatment clinical responses in 261 canine lymphoma patients scheduled to receive at least 1 of 5 common chemotherapy agents (doxorubicin, vincristine, cyclophosphamide, lomustine and rabacfosadine). We used flow cytometry analysis for immunophenotyping and ex vivo chemosensitivity testing. For each drug, 70% of treated patients were randomly selected to train a random forest model to predict the probability of positive Veterinary Cooperative Oncology Group (VCOG) clinical response based on input variables including antigen expression profiles and treatment sensitivity readouts for each patient's cancer cells. The remaining 30% of patients were used to test model performance. Most models showed a test set ROC‐AUC > 0.65, and all models had overall ROC‐AUC > 0.95. Predicted response scores significantly distinguished (P < .001) positive responses from negative responses in B‐cell and T‐cell disease and newly diagnosed and relapsed patients. Patient groups with predicted response scores >50% showed a statistically significant reduction (log‐rank P < .05) in time to complete response when compared to the groups with scores <50%. The computational models developed in this study enabled the conversion of ex vivo cell‐based chemosensitivity assay results into a predicted probability of in vivo therapeutic efficacy, which may help improve treatment outcomes of individual canine lymphoma patients by providing predictive estimates of positive treatment response. 相似文献
73.
74.
Sung Mi Kim Eun Hye Lee Se Ryun Kwon Sun Joung Lee Sung Koo Kim Yoon Kwon Nam Ki Hong Kim 《Aquaculture (Amsterdam, Netherlands)》2006,260(1-4):21-26
The recombinant β-tubulin of Pseudocohnilembus persalinus was investigated to test whether tubulin could be a suitable antigenic target against scuticociliatosis. The cDNA of P. persalinus β-tubulin has an open reading frame of 1332 bp and had 15 TAA triplets code for glutamine (Q). All 15 TAA/Q triplets of the β-tubulin cDNA were mutated to universal CAA/Q triplets by site-directed mutagenesis and the mutated β-tubulin was expressed in Escherichia coli as a fusion with glutathione S-transferase (GST-BTU). In Western blot analysis, rat sera immunized with recombinant GST-BTU reacted with reduced β-tubulin of P. persalinus. The immunized rat sera showed higher parasiticidal activity than those of control. Ciliates preincubated with heat-inactivated immune sera showed significantly lower proliferation than ciliates preincubated with control sera when exposed to naïve rat sera as a complement source. The present results suggest that the recombinant β-tubulin of scuticociliates may be used as a target antigen for development of vaccine against scuticociliatosis. 相似文献
75.
Avipoxvirus is known to be endemic in New Zealand and it is a cause of ongoing mortalities in the endangered black robin and shore plover populations. There is no information on the strains of avipoxvirus occurring in New Zealand and their likely origin or pathogenicity. This study was designed to identify the phylogenetic relationships of pathogenic avipoxvirus strains infecting introduced, native, and endemic bird species in New Zealand. Avipoxvirus 4b core protein gene was detected in tissue samples from 25/48 birds (52.1%) from 15 different species in New Zealand. Bootstrap analysis of avipoxvirus 4b core protein gene revealed that the New Zealand avipoxvirus isolates comprised of three different subclades. The majority of New Zealand avipoxvirus isolates (74%) belonged to A1 subclade which shared 100% genetic similarity with the fowlpox HPB strain. An isolate from a wood-pigeon (kereru) belonged to subclade A3, displaying 100% sequence homology to albatrosspox virus. An additional group, isolated from two shore plovers and one South Island saddleback, grouped within subclade B1 and presented 99% sequence homology to European PM33/2007 and Hawaiian HAAM 22.10H8 isolates. The results suggest that a variety of New Zealand bird species are susceptible to avipoxvirus infection, that there are more than two distinctive avipoxvirus subclades in New Zealand, and that the most prevalent A1 strain may have been introduced to New Zealand through introduced avian hosts such as passerines or poultry. 相似文献
76.
Yeong Ju Kim Ji Hee Kim Jin Hur John Hwa Lee 《Canadian journal of veterinary research》2010,74(1):59-64
Escherichia coli was isolated from the feces of 122 piglets with diarrhea on 55 farms in Korea. The virulence genes of each isolate were characterized by polymerase chain reaction (PCR). Of the 562 isolates, 191 carried 1 or more of the virulence genes tested for in this study. Of the 191 isolates, 114 (60%) carried 1 or more of the genes for enterotoxigenic E. coli (ETEC) fimbriae F4, F5, F6, F18, and F41 and ETEC toxins LT, STa, and STb, 57 (30%) carried 1 or more of the genes for the Shiga-toxin-producing E. coli (STEC) toxins Stx1, Stx2, and Stx2e, and 21% and 37% carried the gene for enteropathogenic E. coli intimin and for enteroaggregative E. coli toxin, respectively. Collectively, our results indicate that other pathotypes of E. coli as well as ETEC can be strongly associated with diarrhea in piglets. In addition, detection of the genes for Stx1 and Stx2 indicates that pigs are reservoirs of human pathogenic STEC. 相似文献
77.
78.
Cheunsoon Ahn Hye-Ja Yoo Hye Ja Lee Jung Hee Kim Kyung-Hun Song Jeon Sook Rhie 《Fibers and Polymers》2005,6(1):28-34
The purpose of the research was to examine the effect of three different levels of enzyme treatment on the physical characteristics
and the end-use suitability of the lyocell fabrics made with four different types of commercial wood pulp. The appropriate
enzyme concentration for obtaining an optimum as well as consistent physical characteristics and fabric hand trait was 3 g/l for the concentration levels tested in the present investigation. Weight loss was more affected by higher enzyme concentration
than other physical properties. H2 was least affected by enzyme treatment for all three physical properties and fabric hand.
5 g/l concentration exhibited little difference from 3 g/l in the physical characteristics, whereas the KES-FB values indicated a significant loss of fabric hand in most PHVs with
the 5 g/l concentration level. Among different garment parameters, all four fabric types were relatively inappropriate for the men’s
slacks (MS) fabric due to the lower hand value of koshi required by the MS parameter. However, despite the relatively low
koshi values, high fukurami values required for men’s dress shirt (MWDS) resulted in the highest THV among the four garment
parmeters. The four fabric types, which represent the usage of four different wood pulps, in general seem to exhibit a higher
applicability to women’s winter thin dress (WWTD) than women’s winter suit (WWS) garment parameter. 相似文献
79.
Hye Min Jung Eun Mi Lee Byung Chul Ji Sung Ok Sohn Han Do Ghim Hyunju Cho Young A Han Jin Hyun Choi Jae Deuk Yun Jeong Hyun Yeum 《Fibers and Polymers》2006,7(3):229-234
Poly(vinyl acetate) (PVAc)/poly(vinyl alcohol)(PVA)/montmorillonite (MMT) clay nanocomposite microspheres with a core/shell
structure have been developed via a suspension polymerization approach. In order to prepare the PVAc/MMT and PVAc/PVA/MMT
nanocomposite microspheres, which are promising precursor of PVA/MMT nanocomposite microspheres, suspension polymerization
of vinyl acetate with organophilic MMT and heterogeneous saponification were conducted. A quaternary ammonium salt, cetyltrimethylammonium
bromide, was mixed with the MMT in the monomer phase prior to the suspension polymerization. The rate of conversion decreased
with an increase in MMT concentration. The incorporation of MMT into the PVAc was verified by FT-IR spectroscopy. Organic
vinyl acetate monomers were intercalated into the interlayer regions of organophilic clay hosts and followed by suspension
polymerization. Partially saponified PVA/MMT nanocomposite microspheres with a core/shell structure were successfully prepared
by heterogeneous saponification. 相似文献
80.
Hai Thi Hong Truong Jeong Ho Kim Myoung Cheoul Cho Soo Young Chae Hye Eun Lee 《European journal of plant pathology / European Foundation for Plant Pathology》2013,135(2):289-297
Phytopthora root rot in pepper (C. annuum) is caused by Phytophthora capsici L., which exhibits a high level of pathogenic diversity. Resistance to this disease is conditioned by a number of quantitative trait loci. Pyramiding resistance alleles is desirable and could be simplified by the use of molecular markers tightly linked to the resistance genes. The purpose of this study was development of molecular markers linked to Phytophthora root rot resistance. An F8 recombinant inbred line (RIL) population derived from a cross between YCM334 and a susceptible cultivar ‘Tean’ was used in combination with bulk segregant analysis utilizing RAPD and conversion of AFLP markers linked to Phytophtora root rot resistance into sequence-characterized amplified region (SCAR) markers. In conversion: one marker was successfully converted into a co-dominant SCAR marker SA133_4 linked to the trait. In bulked segregant analysis (BSA): three RAPD primers (UBC484, 504, and 553) produced polymorphisms between DNA pools among 400 primers screened. Genetic linkage analysis showed that the SCAR and RAPD markers were located on chromosome 5 of pepper. Quantitative trait locus (QTL) analysis showed that the SA133_4 and UBC553 were linked to Phytophtora root rot resistance. These markers were correctly identified as resistant or susceptible in nine promising commercial pepper varieties. These markers will be beneficial for marker-assisted selection in pepper breeding. 相似文献