Hydraulic Retention Time Influence on Improving Flocculation in the Activated Sludge Processes Through Polyelectrolytes

Hydraulic retention time (HRT) influence improving sludge flocculation with adding the polyelectrolytes (non-ionic, anionic, and cationic) was studied on an activated sludge (AS) system fed with synthetic domestic wastewater (SDW), dairy industry wastewater (DIW), and caramel industry wastewater (CIW). The sludge volumetric index, food/microorganism ratio (F/M), and mixed liquor volatile suspended solids at different HRTs (6, 8 and 10 h) were monitored on an experimental model. Results showed that both SDW and IW had the best sludge flocculation conditions at 8 h and 100 mL of non-ionic polyelectrolyte (0.2 mg L^?1). In addition, this phenomenon reached the organic matter removal efficiencies of 95.9, 95.7, and 94.2% for SDW, DIW, and CIW, respectively. Therefore, optimum HRT increased the organic matter removal efficiencies by 10%, sludge concentration by 37% (22–55%), and F/M ratio by 70%. Moreover, the polyelectrolytes used in AS improved the sludge flocculation by 2.9 times.     

Effects of seasonal and environmental changes on aquaculture production in tropical Lake Volta,Ghana

Globally, aquaculture production is faced with numerous challenges, notable among which is water quality. The study determined seasonal environmental changes in Lake Volta and its implications on cage production of Nile tilapia, Oreochromis niloticus in experimental and commercial farm (Sun Woo Farm) set-ups. For each set-up, three cages were used, each measuring 81 and 162.5 m³ for experimental and commercial, respectively. The cages were stocked with 22.5 g fingerlings at a rate of 40 and 80 m^?3 in experimental and commercial cages, respectively, and fed commercial diet. The growth performance, yield, economics, and selected water quality parameters were monitored during the dry and wet seasons between November 2014 and July 2015. There were no differences in water quality parameters between dry and wet seasons (p?>?0.05). Water quality for both seasons remained within limits required for good Tilapia growth in both set-ups. There were minimal variations in growth characteristics between seasons which were not significant; however, final mean weight and yields were high in the dry seasons in both set-ups. The cost of feed and fingerling accounting for about 80% of total costs were the major components identified to affect cost of production. A higher profit index and returns on investment were observed in the dry season in both set-ups and was linked to higher survival rates in the season. Production of Nile tilapia in cages can be cultured throughout the year without any adverse effects as exhibited in similar production patterns in both experimental and commercial set-ups.     

Within-stem variations in mechanical properties of <Emphasis Type="Italic">Melia azedarach</Emphasis> planted in northern Vietnam

Within-stem variations in the mechanical properties of 17–19-year-old Melia azedarach planted in two sites in northern Vietnam were examined by destructive and nondestructive methods. Wood samples were collected from 10, 50, and 90% of the radial length from pith on both sides (North and South) at 0.3, 1.3, 3.3, 5.3, and 7.3 m heights above the ground. The mean values in whole trees of wood density (WD), modulus of rupture (MOR), modulus of elasticity (MOE), and dynamic modulus of elasticity (E_d) at 12% moisture content were 0.51 g/cm³, 78.58 MPa, 9.26 GPa, and 10.93 GPa, respectively. Within the stem, the radial position was a highly (p?<?0.001) significant source of variation in mechanical properties. MOR, MOE, and E_d increased from pith to bark. WD had a strong positive linear relationship with both MOR (r?=?0.85, p?<?0.001) and MOE (r?=?0.73, p?<?0.001). This suggests that it is potentially possible to improve mechanical properties through controlling WD. MOR had also a strong linear relationship with E_d (r?=?0.84, p?<?0.001). This indicates that E_d is a good indicator to predicting the strength of wood if the density of measured element is known. Besides, the stress wave method used in this study provides relatively accurate information for determining the stiffness of Melia azedarach planted in northern Vietnam.     

Neutral models for testing landscape hypotheses

Neutral landscape models were originally developed to test the hypothesis that human-induced fragmentation produces patterns distinctly different from those associated with random processes. Other uses for neutral models have become apparent, including the development and testing of landscape metrics to characterize landscape pattern. Although metric development proved to be significant, the focus on metrics obscured the need for iterative hypothesis testing fundamental to the advancement of the discipline. We present here an example of an alternative neutral model and hypothesis designed to relate the process of landscape change to observed landscape patterns. The methods and program, QRULE, are described and options for statistical testing outlined. The results show that human fragmentation of landscapes results in a non-random association of land-cover types that can be describe by simple statistical methods. Options for additional landscape studies are discussed and access to QRULE described in the hope that these methods will be employed to advance our understanding of the processes that affect the structure and function in human dominated landscapes.     

Immunogene expression in head kidney and spleen of common carp (<Emphasis Type="Italic">Cyprinus carpio</Emphasis> L.) following thermal stress and challenge with Gram-negative bacterium, <Emphasis Type="Italic">Aeromonas hydrophila</Emphasis>

To evaluate the effect of thermal and microbial stress on the immune response of common carp (Cyprinus carpio L.), relative mRNA expression level of pro-inflammatory cytokines [tumor necrosis factor alpha (TNF-α) and interleukin (IL)-1β] and other genes related to immune or stress response [inducible nitric oxide synthase (iNOS), heat shock protein 70 (Hsp70), superoxide dismutase one (SOD1), and glucocorticoid receptor (GR)] was measured by quantitative PCR (qPCR). In addition, total protein and total immunoglobulin level in blood plasma of experimental common carp was also assayed. All the above parameters were estimated 24 h post-challenge with Gram-negative bacterium, Aeromonas hydrophila. Common carp (54.89?±?6.90 g) were initially exposed to 20 °C (control group) and 30 °C (thermal stress group) water temperature for 30 days, followed by experimental challenge with 2.29?×?10⁸ colony forming unit/mL (CFU/mL; LD₅₀ dose) of A. hydrophila. Exposure of fish to thermal stress and subsequently challenge with A. hydrophila significantly (P?<?0.05) increases the IL-1β mRNA expression in head kidney and spleen of common carp by ~?39.94 and ~?4.11-fold, respectively. However, TNF-α mRNA expression in spleen decreased ~?5.63-fold in control fish challenged with A. hydrophila. Thermal stress and challenge with bacterium suppresses the iNOS and GR mRNA expression in spleen of common carp. Moreover, significant (P?<?0.05) increase in total protein content of blood plasma (~?43 mg/g) was evident in fish exposed to thermal stress and challenged with A. hydrophila. In conclusion, our study highlights the importance of elevated temperature stress and microbial infection in differential regulation of expression of several immunogenes in common carp.     

Effects of nitrogen fertilizer application rates on nitrate nitrogen distribution in saline soil in the Hai River Basin,China

Background and Objectives  

Soil nitrate nitrogen (NO₃ ⁻-N) accumulation is related closely to NO₃ ⁻ leaching, which is an important issue in groundwater pollution, especially in intensive agricultural areas with saline soils where volumes of water are used in irrigation to avoid salt accumulation in the root zone. However, in the saline environment in Hai River Basin, China, the importance of detailed research into NO₃ ⁻-N distribution in the root zone has not been adequately recognized. Considering the impacts of eco-environmental system N and crop production, the present study aimed at contributing to an understanding of the effects of N application rate on soil NO₃ ⁻-N distribution, NO₃ ⁻-N residue, N loss, and maize (Zea mays L.) yield in this region.     

Carbon inventory methods and carbon mitigation potentials of forests in Europe: a short review of recent progress

The role of European forests and forest management in the carbon balance has received much attention in research recently. This was particularly motivated by the recognition of forest management as one possible measure countries may adopt in the framework of the Kyoto Protocol to reduce the concentration of greenhouse gases in the earth’s atmosphere. The main method to assess carbon budget in forests is based on traditional forest inventories. This method requires the conversion of measured stem volume to carbon pools. This conversion has been identified as a large source of uncertainty in past assessments. Over the last 5 years, intensive research efforts have resulted in significant advances in the reliability of forest inventory based carbon budgets. In parallel, the impact of forest management on the carbon balance of forest ecosystems has been investigated and the carbon mitigation potential of these activities has been analysed. This paper reviews the progress that was made in these two fields of research with a particular focus on European forests.

A review of seed production and stock enhancement for commercially important portunid crabs in Japan

We reviewed the present status of seed production for stock enhancement and evaluated the impact of stocking on commercial catches in Japan, of portunid crabs particularly Portunus trituberculatus and Scylla paramamosain. The mean survival rate from hatching to first-stage crabs was around 10%, and 20–30% of the larval culture trials conducted in recent years could not harvest juveniles during seed production. To achieve reliable mass seed production technologies, measures for controlling disease and the nutritional condition of larvae in seed production tanks are required to be developed. The main spawning season extends from April/May to July. Reflecting their life cycle characteristics such as high growth rates, crabs recruit to the fishery after September and largely contribute to the commercial landings until December in the hatching year. The main release season of juveniles is from June to July. Consequently, released juveniles are expected to contribute to commercial landings in the release year. Analyses of catch and release statistics in two small bays estimated the yield from released individuals (YPR, yield per release) at 2.4 g for P. trituberculatus and 3.3–7.7 g for S. paramamosain, which were similar to values reported from tagging surveys. On a major regional basis, catch and release histories of P. trituberculatus highlighted the impact of hatchery releases on commercial landings as 33.6 g YPR in the Seto Inland Sea, where catches and releases have been greatest. Although the YPR estimates were different between small bays and major regional seas, Japanese stock enhancement programmes should have had an impact on portunid crab production, dependent on the magnitude of the releases.     

Phytochemical Profile,Antioxidant and Cytotoxic Activities of the Carob Tree (<Emphasis Type="Italic">Ceratonia siliqua</Emphasis> L.) Germ Flour Extracts

This work aimed to evaluate the phytochemical content and to determine the antioxidant and cytotoxic activities of methanol extracts of the carob tree (Ceratonia siliqua L.) germ flour. The extracts were rich in phenolic compounds, had considerable antioxidant activity, and reduced the viability of cervical (HeLa) cancer cells. The chemical content and the biological activities of the extracts were significantly affected by gender and cultivar. Female cultivar Galhosa had the highest levels of phenolic compounds, and the highest antioxidant activity. Extracts from the hermaphrodite trees and from the female cultivars Galhosa and Costela/Canela exhibited the highest cytotoxic activity. The most abundant compound was theophylline. The phenolic content was correlated to both antioxidant and cytotoxic activities. Our findings provide new knowledge about the health implications of consuming food supplemented with carob germ flour.     

Ovicidal activity of seven <Emphasis Type="Italic">Pochonia chlamydosporia</Emphasis> fungal isolates on <Emphasis Type="Italic">Ascaris suum</Emphasis> eggs

The ovicidal effect of the nematophagous fungus Pochonia chlamydosporia on eggs of Ascaris suum was tested under laboratory conditions. A. suum eggs were plated on 2% water–agar with seven fungal isolates (Isol. 5, Isol. 31, Isol. 1, VC1, Isol. 12, Isol. 22 and VC4) and control without fungus. After 5, 7, 10, 14, 15 and 21 days of incubation, approximately 100 eggs were removed from the plates and classified according to the following parameters: type 1, biochemical and physiological effect without morphological damage to the eggshell, type 2, lytic effect with morphological alteration of the eggshell and embryo and type 3, lytic effect with morphological alteration of eggshell and embryo showing hyphal penetration and internal egg colonization. The isolates effectively destroyed A. suum eggs and all types of effects were observed during the experiment. There was no variation in ovicidal capacity (type 3 effect) among the isolates (p > 0.05) throughout the experiment. After 21 days, isolate 5 showed the highest percentages of type 3 effect (58.33%). The results indicated that P. chlamydosporia (Isol. 5, Isol. 31, Isol. 1, VC1, Isol. 12, Isol. 22 and VC4) can destroy A. suum eggs and is, therefore, a potential biological control agent of nematodes.