全文获取类型
收费全文 | 20196篇 |
免费 | 1118篇 |
国内免费 | 2102篇 |
专业分类
林业 | 2075篇 |
农学 | 2313篇 |
基础科学 | 1314篇 |
2540篇 | |
综合类 | 7597篇 |
农作物 | 1357篇 |
水产渔业 | 869篇 |
畜牧兽医 | 3228篇 |
园艺 | 1056篇 |
植物保护 | 1067篇 |
出版年
2024年 | 82篇 |
2023年 | 238篇 |
2022年 | 609篇 |
2021年 | 757篇 |
2020年 | 659篇 |
2019年 | 642篇 |
2018年 | 467篇 |
2017年 | 742篇 |
2016年 | 653篇 |
2015年 | 832篇 |
2014年 | 972篇 |
2013年 | 1208篇 |
2012年 | 1509篇 |
2011年 | 1523篇 |
2010年 | 1526篇 |
2009年 | 1337篇 |
2008年 | 1349篇 |
2007年 | 1368篇 |
2006年 | 1175篇 |
2005年 | 996篇 |
2004年 | 635篇 |
2003年 | 542篇 |
2002年 | 745篇 |
2001年 | 643篇 |
2000年 | 470篇 |
1999年 | 345篇 |
1998年 | 207篇 |
1997年 | 174篇 |
1996年 | 159篇 |
1995年 | 129篇 |
1994年 | 128篇 |
1993年 | 110篇 |
1992年 | 102篇 |
1991年 | 116篇 |
1990年 | 62篇 |
1989年 | 61篇 |
1988年 | 44篇 |
1987年 | 28篇 |
1986年 | 16篇 |
1985年 | 8篇 |
1984年 | 7篇 |
1983年 | 5篇 |
1982年 | 6篇 |
1981年 | 8篇 |
1980年 | 4篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1962年 | 4篇 |
1956年 | 1篇 |
1955年 | 2篇 |
排序方式: 共有10000条查询结果,搜索用时 750 毫秒
71.
ZHOU Shu-lu YE Ren-gao LIU Xiao-bo ZHANG Hong XU Han-shi DU Yong LI You-ji YANG Nian-sheng YANG Xiao YU Xue-qing 《园艺学报》2003,19(6):782-785
AIM:To detect the association between the polymorphism of Fc receptor γ chain gene at position-29 in promoter and systemic lupus erythematosus(SLE).METHODS:The genotypes at position -29 in promoter of Fc receptor γ chain gene were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 180 patients with SLE and 140 ethnically matched controls in southern China.RESULTS:The frequencies of TT genotype(33.3%) and T allele (54.4%) at position -29 in patients with SLE were significantly higher than those in controls (17.2% and 42.9%, respectively), whereas, the frequencies of GG genotype (24.4%) and G allele (45.6%) in patients with SLE were remarkably lower than those in controls (31.4% and 57.1%, respectively) (P<0.05). The TT genotype and T allele at position -29 were not associated with lupus nephritis in SLE patients (P>0.05).CONCLUSION:Our results indicate that the T allele at position -29 in promoter of Fc receptor gene probably contributes to the susceptibility to SLE, but does not play a role in the occurrence of lupus nephritis. 相似文献
72.
AIM: To investigate the application of atomic force microscope (AFM) in clinic diagnosis. METHODS: Topographic images and some parameters of large range field and microstructures of erythrocytes in the blood of normal subjects, lung cancer and myelodisplastic syndrome (MDS) patients were examined by atomic force microscope. RESULTS: Many clear topographic images of many erythrocytes, single erythrocyte, and microstructure of erythrocyte membrane surface were obtained. Many erythrocytes in lung cancer patients were found to change into echinocytes. One erythrocyte had 10-20 protuberances, most of which, with a mean width of 589. 0 nm and a length of 646. 7 nm, were on the edge of cells. The protuberances on the center of echinocytes are lodged and embedded. The erythrocytes of MDS patients were biconcave in shape. Many apertures with different diameters of tens to hundreds nanometer appeared on the surface of cell membrane. CONCLUSIONS: AFM can be widely applied in clinic pathological inspection, including quantification of cells, obtainment and comparison of many parameters (such as diameter, thickness, volume, surface, surface area/volume ratio), observation of topograph of single cell, and observation and comparison of membrane surface microstructure of cells, and so on. 相似文献
73.
AIM:To study the effect of TGF-β1 and TNF-α antisense PS-ODNS on ex vivo expansion of hematopoietic stem/progenitor cells (HSPC). METHODS:CD34+cells were purified from fresh umbilical cord blood by immunomagnetic beads, and mononuclear cells were purified from bone marrow by Ficoll-hypaque. The effects of TGF-β1 and /or TNF-α antisense PS-ODNS on ex vivo expansion of CD34+ cells、CFU-GEMM、CFU-GM、CFU-E and BFU-E were detected by using liquid and semi-solid culture systems.RESULTS:TGF-β1 antisense PS-ODNS cooperated with cytokines increased the number of CD34+ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E, which was as 4, 2.6, 2.7, 1.8, 2.1 times as that of the control (the cytokines combination), respectively. TNF-α antisense PS-ODNS cooperated with cytokines respectively increased the number of CD34+ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E by 4, 2.9, 2.6, 1.7, 1.8 times as that of the control. The above two antisense PS-ODNS cooperated with cytokines could respectively increased the number of CD34+ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E by 5.3, 2.1, 2.7, 1.9, 1.8 times as that of the control.CONCLUSION:Inhibition of endogenous TGF-β1 and TNF-α by antisense PS-ODNS will be one of the effective methods to expand HSPC ex vivo. 相似文献
74.
75.
76.
77.
78.
79.
亚硫酸氢钠处理减轻低温对温州蜜柑光合作用的影响 总被引:12,自引:0,他引:12
低温胁迫使温州蜜柑叶片的净光合速率(Pn)、光系统Ⅱ的光化学效率(Fv/Fm)及光合电子传递速率(ETR)下降,反映跨膜质子动力势的叶绿素毫秒延迟发光(ms-DIE)减弱,叶片中的ATP含量降低。低温胁迫前,用NaHSO3 5 mmol/L涂于叶片表面,可使处理植株叶片的Pn和Fv/Fm分别少下降了11.5%和11.6%,ETR和ATP含量几乎没有下降,ms-DLE的下降幅度减少。可见,在柑橘上施用NaHSO3能够减轻短期低温对光合机构及光合作用的影响。 相似文献
80.