Dynamic Analysis of Nitric Oxide and Total Oxidant Capacity in Cow Uterine Secretion with Subclinical Endometritis

Subclinical endometritis is a physiological inflammation that serves to clear persistent contaminants from the uterus. To investigate the alteration of antioxidant, such as vitamin E(VE) and vitamin C(VC), total oxidant capacity(TOC) and nitric oxide(NO) in cows with normal and subclinical endometritis(SCE), we examined the concentrations of NO, VC and VE, TOC and polymorphonuclear neutrophils(PMN) percentage in uterine secretion. The cows were divided into two groups, normal(n=20) and subclinical endometritis(SCE, n=60), based on endometrial cytology(presence of PMN≥5%). Uterine secretion and blood were collected as described previously. Griess reaction was used to determine the concentration of NO. The concentrations of TOC, VC and VE were detected by a commercially available assay kit. The results showed that the concentrations of NO, TOC and PMN percentage were significantly higher(P0.01, P0.05, and P0.01, respectively) in uterine secretion with SCE compared to those from normal; however, the levels of VC and VE were significantly lesser(P0.01). In conclusion, the concentrations of NO, TOC, VC, VE and PMN percentage differed between normal and SCE cows. Meanwhile, the relationship between the concentration of NO and PMN percentage from uterine secretion in cows with subclinical endometritis were positively correlated. Consequently, these alterations in NO, TOC, VC, VE levels and PMN percentage contributed to as a diagnostic index of the uterine inflammation, with the aim to increase the reproduction of the cows and the decrease economic losses.     

论《孝经》对“中韩”道德发展及其社会现代化转型的意义

《孝经》及其蕴涵的孝道孝德是中国传统文化的文明精髓之一,也是中国传统道德的核心内容。它不仅形塑了中国古代的道德文化,化育了中国人的人格品质和行为范式,而且深深地影响了中国的近邻尤其是东亚的朝鲜民族的精神风貌和道德取向,成为中国和韩国在社会现代化转型过程中中和西方个体本位价值观念的冲击,克服现代文明病,构建社会和谐的重要精神资源。     

Expression,Purification, and Functional Analysis of Three Low‐Molecular‐Weight Glutenin Subunits from Wheat Cultivar Cheyenne

Glutenins, which form the network of gluten protein, are of great importance for the quality of flour products. Glutenins can be divided into HMW and LMW subunits according to molecular weight. Three genes for LMW glutenin subunits (LMW‐GS), named lmw‐cnd1, lmw‐cnd2, and lmw‐cnd3 with open reading frames of 1,053, 903, and 969 bp, respectively, were cloned from wheat cultivar Cheyenne. Heterologous expression vectors of the three LMW‐GS were constructed, and the recombinant proteins LMW‐CND1, LMW‐CND2, and LMW‐CND3 were overexpressed in Escherichia coli. After cell disruption with ultrasound, target proteins of high purity were obtained by using Ni²⁺ affinity chromatography. Farinograph and TAPlus measurements were used to investigate the effects of the three LMW‐GS on the characteristics of flour and dough. The results showed that the addition of each LMW‐GS can lead to an increase in the elasticity of the dough. Moreover, LMW‐CND2 and LMW‐CND3 promoted the strength of the dough. All three LMW‐GS caused a decrease of hardness and increase of springiness and cohesiveness of dough according to texture profiling results. Consequently, all three LMW‐GS have positive effects on the processing characteristics of dough and can improve bread quality to different extents.     

大豆光合生理生态的研究——第15报 大豆不同品种光合性状和固氮能力的比较

对大豆20个品种的光合性状和固氮能力研究表明,大多数新品种或高产品种植株中上部叶片较老品种具有较多的栅栏细胞层数和细胞及叶绿体数目;较大的叶厚度和比叶重;在生殖生长期具有较高的光合速率。同时大多数新品种具有较高的根瘤固氮酶活性和较大的幼茎段酰脲含量。大豆叶片光合速率和根瘤固氮酶活性及幼茎和荚皮酰脲含量均呈显著正相关。新品种与老品种相比具有较高的光合和固氮能力。     

逐步判别分析在中国烤烟香型鉴定中的应用

分析云南、河南、辽宁烤烟的多项化学成分含量,并对烤烟进行专家评吸香型鉴定。以化学成分为自变量对不同香型烤烟进行逐步判别分析,构建判别函数,并对判别函数的判别效果进行检验。结果表明,总糖、还原糖、烟碱、钾等8个变量进入判别函数,由判别函数的方差分析可知判别函数具有显著意义;用自身验证法和交互验证法对原样品进行回判,回判的准确率分别达95.5%和94.3%,新样品的判别准确率达93.3%,判别效果良好,在实际应用中值得推广。     

仔犬肝脓肿的诊治

2006年昆明某犬场5窝37头刚出生5至20日龄仔犬，其中9头仔犬出现食欲废绝，精神不佳，呼吸困难等症状，治疗后5头死亡，死亡率55．6%。经解剖分析，仔犬死于肝脓肿。笔者现将仔犬的临床病理特征及治疗经验叙述如下：     

安徽省退耕还林工程总体构想与实施对策

安徽省实施退耕还林工程，是优化配置土地资源，改善生态环境质量，促进社会经济可持续发展的根本和切入点，意义重大，影响深远。通过全面分析安徽省实施退耕还林工程的目的意义和建设条件，提出了全省2002年退耕还林工程建设总体构想，并指出保障退耕还林工程顺利进展的对策。     

哈尔滨地区犬旋毛虫对猪,犬的感染性研究

用消化法所得的犬旋毛虫蚴分别接种猪，犬。结果表明：犬旋毛虫对猪、犬的感染性存在着明显差异，在犬的繁殖力指数为１８．６８。而在猪为零，说明哈尔滨地区犬旋毛虫相当于本地毛形线虫。犬旋毛虫对犬易感而对猪不感染，通信能通过猪的感染而对人体健康构成威胁。     

鸡静注,肌注及内服氟哌酸的药物动力学研究

选用４２只健康ＡＡ鸡，分３组（每组１４只）进行药物动力学研究：静注、肌注及内服的剂量均为１０ｍｇ／ｋｇ。用二氯甲烷提取血浆中的药物，反相高效液相色谱法测定血浆氟哌酸的浓度，测定时以吡哌酸为内标。所得药物一时间数据用ＭＣＰＫＰ计算机程序处理，静注给药拟合二室开放模型，主要动力学参数是：ｔ１／２α０．１４±０．０８ｈ，ｔ１／２β３．６５±１．０３ｈ，Ｋｅ０．８８±０．４０ｈ－１，Ｖｄ３．８１±０．９８Ｌ／ｋｇ，ＡＵＣ１４．１４±３．２９μｇ／ｍｌ．ｈ。内服和肌注给药均适合具有一级吸收二室开放模型，肌注的主要动力学参数是：ｔ１／２ｋａ０．２２±０．１０ｈ，ｔ１／２α１．０３±０．６５ｈ，ｔ１／２β６．８７±２．８６ｈ，Ｋｅ０．２８±０．１０ｈ－１，ＡＵＣ９．８６±２．８８μｇ／ｍｌ．ｈ，Ｆ６９．７８％。内服的主要动力学参数是：ｔ１／２ｋａ０．６９±０．２３ｈ，ｔ１／２α１．７６±０．８９ｈ，ｔ１／２β１０．４３±３．２２ｈ，Ｋｅ０．２１±０．０５ｈ－１，ＡＵＣ８．５６±１．３６μｇ／ｍｌ．ｈ，Ｆ６０．５２％。鸡静注、肌注、内服氟哌酸的药动学特征是吸收较快，在体内分布广泛、半衰期较长。     

贵州天然豆科牧草——窄叶野豌豆的引种与驯化

对采自贵州省册亨县、三都县的窄叶野豌豆野生种进行了引种驯化。结果表明：其产草量中等，鲜草３７．５～４５ｔ／ｈｍ２，草质优良；生育期短，早熟；种子产量高，收纯净种子５００～６００ｋｇ／ｈｍ２。适合在我国南方中亚热带地区同水稻、玉米轮作，也可作为农田填闲作物栽培