Cutaneous lymphoma in a juvenile dog

Abstract: An 18-month-old male Doberman Pinscher was referred to the Veterinary Medical Teaching Hospital of the College of Veterinary Medicine for an erythemic nodular mass on the right forelimb. The mass was diagnosed as cutaneous lymphoma, based on cytologic examination of a mass aspirate and histopathology. Using immunohistochemistry the neoplastic cells were positive for CD3 but negative for CD79a, E-cadherin, and pancytokeratin, confirming their origin as T lymphocytes. No tumor recurrence was noted 18 months after surgery. To our knowledge, this is the first report of a solitary nodular form of cutaneous lymphoma in a young dog.     

生物炭基肥对马铃薯田土壤脲酶活性和产量的影响

为明确生物炭基肥在马铃薯生产上的应用效果,以马铃薯品种中薯3号为材料,采用随机区组设计,研究大田条件下,0（CK₀）、300（T₁）、600（T₂）、900（T₃）、1200（T₄）kg/hm²生物炭基肥及与之等量N、P、K化肥（分别为CK₁、CK₂、CK₃、CK₄）对马铃薯田土壤脲酶活性和产量的影响。结果表明,在马铃薯苗期,土壤脲酶活性表现为CK₄>T₄>CK₃>T₃>CK₂>T₂>CK₁>T₁>CK₀;在块茎增长期和成熟收获期,土壤脲酶活性表现为T₄>CK₄>T₃>CK₃>T₂>CK₂>T₁>CK₁>CK₀。马铃薯不同生育时期土壤脲酶活性表现为块茎增长期>成熟收获期>苗期。不同土层脲酶活性表现为0~10cm>10~20cm>20~40cm。马铃薯产量为T₃>T₄>CK₃>T₂>CK₄>T₁>CK₂>CK₁>CK₀。马铃薯产量与各土层脲酶活性均呈正相关,且在块茎增长期和成熟收获期相关性达到极显著水平。生物炭基肥施用量为900kg/hm²时马铃薯产量最高,是适合浙江地区马铃薯种植的推荐施肥量。     

人为活动干扰对甘南高寒草甸喜马拉雅旱獭食性的影响

人为活动会对草地生态系统的组成、过程和功能产生极大影响。明确人为活动干扰对甘南高寒草甸喜马拉雅旱獭(Marmota himalayana)食性的影响,可为其管理提供重要依据。本研究利用粪便显微组织分析法,分析比较了自然生境和两种人为活动干扰生境下喜马拉雅旱獭夏季的食物组成、比例、多样性及其营养生态位的变化。结果表明,喜马拉雅旱獭主要采食杂类草和禾本科植物,不同生境下其采食的植物种类变化不大,采食垂穗披碱草(Elymus nutans)的比例最大(自然生境、干扰生境1和干扰生境2的相对密度分别为46.67%,24.59%和19.00%)。相较自然生境,干扰生境下喜马拉雅旱獭对莎草科和禾本科植物采食相对比例显著降低,而对杂类草的采食相对比例显著增加。人为活动干扰后,喜马拉雅旱獭食物的多样性指数增高,食物生态位宽度增加,拓宽自身食物生态位宽度是喜马拉雅旱獭应对人为活动干扰的重要策略。     

再生稻再生芽萌发期降水量和作物需水量的年际变化特征分析

基于遂宁、内江、泸州、宜宾、纳溪和叙永等6个气象站1951—2007年的逐日气象资料,采用FAO推荐的Penman-Monteith公式计算再生稻作物需水量,并结合同期降水量,以自然降水量/作物需水量(R/ETc)为指标,探讨了川东南地区中稻再生芽萌发期(即7月下旬—8月中旬)的降水量和作物需水量的地区差异特征和年际变化特征。结果表明,遂宁、内江、泸州、纳溪、宜宾和叙永再生芽萌发期多年平均降水量分别为156.7、175.4、168.5、66.7、227.5 mm和153.4 mm。需水量多年平均分别为141.5、138.6、144.5、146.0、128.5 mm和142.7 mm。除了纳溪外,其余各地区再生芽萌发期的降水量均高于其作物需水量。从自然降水量和作物需水量之间的关系上看,宜宾、内江、泸州及叙永均较适合再生稻发展,而纳溪则存在较大的风险。进一步分析内江、泸州再生芽萌发期作物需水量和自然降水量的年际变化特征,发现从1951—2007年,内江降水量与需水量的比值1的年份占了63%,比值在0.85~1之间的概率为3%,比值在0.5~0.7之间的概率为11%,降水量与需水量的比值0.5为12%。从1951—2002年,泸州降水量与需水量的比值1的年份占了52%,比值在0.85~1之间的概率为13%,比值在0.5~0.7之间的概率为21%,降水量与需水量的比值0.5的概率为4%。相对而言,内江中稻再生芽萌发期仍然存在较大的受旱风险。根据以上结果,探讨了川东南再生稻发展适宜区及高产栽培策略,纳溪受旱风险较大不宜晒田,遂宁、宜宾、内江、泸州、叙永均能进行中期晒田。     

新颖袋泡茶工艺参数的优化

研究了原料颗粒大小、浸泡时间以及冲泡次数对新颖袋泡茶品质的影响。结果表明,袋泡茶选择40～60目的颗粒原料最佳,汤色黄绿、浸出率高,冲泡5min、冲泡3次为最佳,3次氨基酸、咖啡碱、茶多酚浸出率分别占到总量的96.74%、96.24%、95.76%。     

Discrimination of intact and injured Listeria monocytogenes by Fourier transform infrared spectroscopy and principal component analysis

Fourier transform infrared spectroscopy (FT-IR, 4000-600 cm(-)(1)) was used to discriminate between intact and sonication-injured Listeria monocytogenes ATCC 19114 and to distinguish this strain from other selected Listeria strains (L. innocua ATCC 51742, L. innocua ATCC 33090, and L. monocytogenes ATCC 7644). FT-IR vibrational overtone and combination bands from mid-IR active components of intact and injured bacterial cells produced distinctive "fingerprints" at wavenumbers between 1500 and 800 cm(-)(1). Spectral data were analyzed by principal component analysis. Clear segregations of different intact and injured strains of Listeria were observed, suggesting that FT-IR can detect biochemical differences between intact and injured bacterial cells. This technique may provide a tool for the rapid assessment of cell viability and thereby the control of foodborne pathogens.     

Assessment of growth and seed oil composition of kenaf (Hibiscus cannabinus L.) germplasm

This study was conducted to evaluate the growth characteristics and fatty acid composition among 15 kenaf mutants derived from the kenaf germplasm C14 and 15 kenaf accessions originating from Russia, India, China, Iran, and Italy. The overall growth performance (plant height, stem diameter, flowering date, leaf, and flower size) of the stem color mutant lines derived from C14 are similar to those of the original variety. However, the flower color mutant lines derived from C14 showed flowering to occur 10 days later when compared with the original variety and showed smaller leaf sizes than the original variety. Late-ripened kenaf accessions (Jinju, Auxu, and Jnagdae) can yield more bio-mass compared with early or medium-maturing germplasm. The late maturity kenaf (Auxu, Jinju, and Jangdae) has a higher oil percentage than the early maturity germplasm. Linoleic, oleic, and palmitic acids were the predominant fatty acids in all kenaf seeds. The stem color mutant lines significantly surpassed the parental means of all saturated fatty acids. In addition, the flower color mutant lines showed broad ranges of variation in oleic acid. The 15 accessions showed a wide range of fatty acid compositions, spanning from 29.75 to 38.30% saturated fatty acids and 61.70 to 70.24% total unsaturated fatty acids, and the late maturity kenaf has a higher linoleic acid percentage than the early maturity germplasm. The flowering period was highly positively (P ≤ 0.01) correlated with the plant height, stem diameter, oil percent, and linolenic acid (C_18:3), and it was significantly negatively (P ≤ 0.01) correlated with stearic acid (C_18:0). These results will provide valuable information to assist the parental selection of kenaf breeding.     

湖南省衡东县杂交晚稻最佳施肥量

通过测土配方施肥对丰源优272的试验数据分析得出,N、P、K对其经济性状都有不同程度的影响,以P增产效应较大,N相对略低,K次之。最佳施肥量为46%尿素298.5 kghm2、12%过磷酸钙312.0 kghm2、60%氯化钾82.5 kghm2,其产量为7 707.0 kghm2,为衡东县水稻生产提供了测土配方施肥的技术依据。      

云南红豆杉当年生枝条分枝特征与其综合表现的关系研究

为了解云南红豆杉当年生枝条分枝特征对其生物量性状和总体表现的影响,探讨药用原料林的当年生枝条分枝特征调控技术。采用主成分分析方法综合评价当年生枝条总体表现,通径分析当年生枝条的主枝枝径、主枝长、一级分枝枝径、一级分枝长、一级分枝数量等分枝特征与其叶生物量、总生物量、叶生物量比以及总体表现等因子间关系,研究其分枝特征调控重点与方向并验证。结果表明：云南红豆杉当年生枝条的系列分枝特征以及生物量性状均有着丰富的变异。当年生枝条的一级分枝数量和枝长对其叶生物量、总生物量,叶生物量比有着极显著性正向作用;当年生枝条的主枝枝径对其叶生物量、总生物量有极显著性正向作用;当年生枝条的主枝枝长对其叶生物量比有显著性负向作用;当年生枝条分枝特征对其生物量性状间的相对重要性以及起主导作用的分枝特征因子有所变化。促进当年生枝条叶生物量和总生物量,增大叶生物量比的分枝特征调控重点与方向明显不同,当年生枝条的一级分枝数量、枝长和主枝枝径对其总体表现有显著性正向作用,主枝长、一级分枝枝径对其总体表现直接作用不显著。药用原料林当年生枝条理想分枝特征如下,首先要有繁多的一级分枝数量,其次一级分枝要长,最后是主枝枝径要粗大。云南红豆杉药用原料林当年生新枝分枝特征的调控重点与方向是增大一级分枝数量。     

“十一五”国家863计划现代农业技术领域回顾与展望

分析回顾了“十一五”以来国家863计划现代农业技术领域从制订规划、组织实施和加强过程管理等方面落实《国家中长期科学和技术发展规划纲要》开展的工作,列举了我国现代农业技术领域在前沿技术研发、科研工作成果产出和人才基地建设等方面取得的成效,分析了《国家中长期科学和技术发展规划纲要》前期落实过程中存在的问题和挑战, 并为今后工作的开展提出了相应的建议。