桃褐腐病菌激活蛋白对草莓幼苗防御酶酶活性的影响

激活蛋白是从多种病原真菌中分离出的一种蛋白激发子,为了解桃褐腐病菌激活蛋白对植物防御酶的诱导作用,本实验通过沸水浴、离心等方法,从桃褐腐病菌（Monilinia fructicola）菌丝体中分离出激活蛋白的粗提物,并喷雾到草莓幼苗的叶片上,测定了部分防御酶和病程相关蛋白的活性变化。结果表明：经桃褐腐病菌激活蛋白处理后,0-18h内草莓叶片的苯丙氨酸解氨酶(PAL)活性呈曲线增高的趋势,18h达到最高值,最高值比对照增加40.7%; 0-6h内草莓叶片过氧化物酶(POD) 和多酚氧化酶（PPO）活性均呈急剧增高的趋势,6h达到最高值,其中POD活性比对照增加126.2%,PPO活性比对照增加62.9%。经激活蛋白处理后草莓叶片的β-1,3-葡聚糖酶、几丁质酶活性也有增高的趋势。     

重庆市南岸区农村居民点整理潜力研究

为得出重庆市南岸区农村居民点整理潜力,对农村集体建设用地流转提供客观依据以及农村居民点用地管理提供理论支撑。采用人均农村居民点建设用地标准测算法对至2020年农村居民点整理潜力进行测算,然后采用闲置宅基地抽样调查法对其进行修正,获得农村居民点整理潜力。根据整理潜力和南岸区实际情况,对农村居民点合理布局进行了规划,并制作2020年重庆市南岸区农村居民点用地分布图。     

水草收割机明轮推进器的建模与仿真分析

为进一步研究水草收割机在乌梁素海生态治理中有效控制湖泊富营养化的问题,针对水草收割机明轮推进器的运动特性,运用MATLAB中SQP法优化明轮的结构尺寸;根据优化的结构尺寸,通过Pro/E软件建立与物理样机相同的明轮推进器三维实体模型,装配后模拟其真实运动情况,并进行干涉检验.通过Pro/E和ADAMS的专用接口模块MECH/Pro将实体模型导入到ADAMS软件中,运用动力学分析软件ADAMS对明轮推进器进行运动仿真,获得有效数据,为水草收割机系列产品设计研究提供参考数据.     

番茄品种耐弱光性的综合评价

研究了苗期弱光条件对幼苗素质，开花，果实大小及产量的影响，采用模糊数学的隶属法对品种的耐弱光性进行评价，并探讨了番茄耐弱光性状的鉴定方法，结果表明，苗期弱光对幼苗素质影响最大，其次影响开花，果实大小和产量，春雷２号和春雷１号属于耐弱光惭弱的品种，白果强丰属不耐弱光品种。     

豇豆胰蛋白酶抑制剂转基因棉花的获得

利用根癌农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍｔｕｍｅｆａｃｉｅｎｓ）介导法将豇豆胰蛋白酶抑制剂（Ｃｏｗ－ｐｅａＴｒｙｐｓｉｎＩｎｈｉｂｉｔｏｒ,ＣｐＴＩ）基因转移进入棉花（ＧｏｓｙｐｉｕｍｈｉｒｓｕｔｕｍＬ．）。棉花幼苗的下胚轴与携带有ＣｐＴＩ基因和Ｎｐｔ－Ⅱ基因的根癌农杆菌共培养,在选择培养基上诱导出了卡那霉素抗性（Ｋｍｒ）愈伤组织。选择Ｎｐｔ－Ⅱ阳性的胚性愈伤组织在胚状体诱导培养基上进行胚状体诱导,继而在分化培养基上进行植株分化,最终获得了再生棉花植株。经Ｎｐｔ－Ⅱ分析、ＰＣＲ及Ｓｏｕｔｈｅｒｎ检测证明,外源ＣｐＴＩ基因和标记基因（Ｎｐｔ－Ⅱ基因）存在于转化棉株及其后代中。抗棉铃虫（Ｈｅｌｉｏｔｈｉｓａｒｍｉｇｅｒａ）生物活性检测表明转基因植株后代具有明显的抗棉铃虫能力。     

乙醛脱氢酶基因（ALDH）转化番茄的研究

乙醛脱氢酶基因（ALDH）为目的基因,构建了pBI-ALDH 植物表达载体,番茄品系03HN31子叶为外植体,经发根农杆菌（Agrobacterium rhizogens）介导采用共培养法转化番茄。通过分子生物学技术（PCR、Southern杂交、RT-PCR）和抗逆相关生理指标测定（相对电导率及丙二醛含量）相结合的方法对转化番茄植株进行检测,研究结果表明：乙醛脱氢酶基因（ ALDH ）导入并整合到番茄的基因组中,在转录水平上可以稳定表达;在干旱、高盐和低温胁迫条件下,转基因番茄植株和对照株的相对电导率和丙二醛含量有差异,证明转化番茄植株的质膜受损程度有所降低,抗氧化胁迫性能有所提高,转化率为10.78%。     

茎芥菜胞质四倍体白菜雄性不育系花药发育的研究

 以茎芥菜胞质雄性不育系与四倍体白菜杂交获得的同源四倍体白菜异源胞质雄性不育系及其保持系为材料,采用形态学和石蜡切片方法研究其花药解剖结构及发育。结果表明：该雄性不育为结构性雄性不育,其退化或畸形雄蕊分为5种类型：盾状雄蕊、条状雄蕊、片状雄蕊、羽状雄蕊和瓣状雄蕊。该雄性不育系花药发育败育有两个时期,盾状雄蕊花药败育于孢原细胞分化期,雄蕊整个发育时期均处在孢原细胞分化期,无绒毡层与花粉母细胞的分化,不形成药室,属孢子体败育型;其它类型雄蕊,花药败育发生在雄蕊原基分化时期,由于雄蕊原基偏离正常的分化轨道,形成瓣状化雄蕊。     

我国秸秆人造板工业的发展现状及前景展望

分析了我国发展农作物秸秆人造板工业的必要性和可行性,概述了我国秸秆人造板工业的发展现状及发展过程中应注意的问题,并对我国秸秆人造板工业的发展前景进行了分析和展望,认为我国的秸秆人造板工业发展前景广阔,并将进入一个快速发展时期.     

优质高产棉花杂交种中棉所101

简要介绍了中棉所101的选育过程、特征特性以及高效栽培技术要点。     

Preliminary study of patients with chronic mountain sickness by GC-TOF-MS based serum metabolomics analysis

AIM: To evaluate specific metabolomics profiles in the serum of patients with chronic mountain sickness (CMS) and to explore the potential metabolic biomarkers in the native Tibetans living on the Qinghai-Tibet Pla-teau.METHODS: A gas chromatography time-of-flight mass spectrometry (GC-TOF-MS) approach as a metabolomics technique was used to evaluate metabolic differences. The native Tibetan CMS patients (n=10) and healthy Tibetan controls (n=10) were enrolled from YuShu in Qinghai province in this study. The serum samples were collected and analyzed by GC-TOF-MS coupled with a series of multivariate statistical analyses such as principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA).RESULTS: The intergroup differences between CMS patients and control subjects have been observed. A list of differential metabolites and several top altered metabolic pathways have been identified. The levels of fumaric acid, an intermediate in the tricarboxylic acid (TCA) cycle, and inosine were highly upregulated in the CMS patients, suggesting a greater effort to hypoxic adaptation in high elevation area. Other differential metabolites, such as methyl phosphate, 2-ketoadipate, lyxose and phytanic acid were also identified. Importantly, the differential metabolites possessed higher area under the ROC curve (AUC) values, indicating an excellent clinical ability for the prediction of CMS. Increased levels of amino acids (isoleucine, glycine, serine, L-cysteine, citrulline and trimethyllysine) were detected in CMS group, yet significantly decreased levels of sulfuric acid, oxamic acid, lyxose and glutamine were also detected in CMS group than those in control group. At the same time, the levels of ribose and glucose-1-phosphate were markedly elevated in CMS group (P<0.05).CONCLUSION: The metabolic activities are significantly altered in the serum of CMS patients. High altitude hypoxia may act on the disturbed glucose metabolism and amino acid metabolism in part of the Tibetan triggered by CMS.