Genetic diversity of winter wheat in Shaanxi province, China, and other common wheat germplasm pools

Cultivated Chinese wheat germplasm has been a valuable genetic resourcein international plant breeding. Landraces endemic to China are a geneticresource that is distinct from other wheat germplasm. Patterns of geneticdiversity among cultivated Chinese accessions and relationship to othergermplasm pools are unknown, despite the proven value and potential novelty. Theobjective of this work was to determine the level of genetic diversity withinimproved Chinese germplasm in the context of several other wheat germplasmpools. We analyzed a set of improved accessions cultivated from the 1940s to the1990s in Shaanxi province, China, using six simple sequence repeat (SSR) primerpairs and 30 restriction fragment length polymorphism - probe enzymecombinations (RFLP-PEC) previously used to characterize 21 geographically basedgermplasm pools. Shaanxi germplasm consists of three groups based on foreignintroductions from Italy, Australia, Denmark, and Russia. There was a decreasein genetic diversity among Shaanxi accessions cultivated in the 1970s and 1980sto the 1990s, and accession classifications based on primary decade ofcultivation were found to be significantly undifferentiated. The analysis of themean genetic distance among 22 geographically based pools of germplasm suggestsseveral regions are significantly undifferentiated. A vast majority of the totalamount of variation was found within pools; therefore, pools appear to belargely differentiated based on small differences in band relative frequency andfew if any unique bands. Previous studies have identified some Chinese landracepools as morphologically and genetically unique. The Shaanxi pool does not havethe same unique morphological or genetic features, nor is it more similar to thelandrace pools than other improved germplasm pools.     

Effects of gaseous pollutants on forests in Eastern North America

The purpose of this paper is to summarize the current state of knowledge concerning direct forest damage caused by atmospheric gaseous pollutants. The major gaseous pollutants affecting forest growth are O₃, SO₂, and HF. Ozone is the most prevalent phytotoxic air pollutant on a regional scale in eastern North America. It has been demonstrated to cause foliar injury and growth effects on trees in both laboratory and field studies. Both SO₂ and HF have been found to cause damage to forests primarily in the vicinity of strong point sources. Case histories of forest damage are reported in the paper which describe foliar and radial growth effects, with associated volume losses. Threshold levels of the gaseous pollutants causing effects on forest growth are provided.     

Heterosis and combining ability for yield and resistance to grain mold in tannin-free photoperiod-insensitive sorghum [Sorghum bicolor (L) Moench] in Senegal

Journal of Crop Science and Biotechnology - Increasing sorghum production per unit area in the farmer’s field by developing high yielding tannin-free and grain mold tolerant varieties in...     

以谷蛋白GluA-2信号肽增强外源蛋白在转基因水稻胚乳中的表达与积累

提高外源蛋白在特定目标组织器官中的表达量是转基因植物研究与开发的核心技术之一。谷蛋白是水稻种子中最主要的贮藏蛋白,其表达具有严格的时空特异性。为进一步研究谷蛋白信号肽序列在指导基因表达中的作用,本研究克隆了水稻谷蛋白Glu A-2基因的启动子及其信号肽编码序列,并与GUS报告基因编码区融合,构建了分别含有和不含有信号肽的表达载体p13GG和p13GSG;经农杆菌介导法分别转入同一水稻品种中,获得了20多个独立转化子,PCR证明外源基因都已整合进了水稻基因组中。Northern杂交结果表明,融合Glu A-2信号肽编码序列可显著提高GUS基因在水稻胚乳中的转录;利用GUS特异的抗体进行Western杂交分析,显示该信号肽序列可显著提高外源蛋白在转基因水稻胚乳中的积累,但是其所指导表达的GUS蛋白在水稻胚乳中并没有表现出相应的活性,其机制有待进一步深入解析。相关结果对于水稻品质改良基因工程研究以及以水稻种子作为生物反应器高效表达外源蛋白具有重要的指导作用。     

Genetic Advancement of Newly Developed Wheat Populations Under Drought-Stressed and Non-Stressed Conditions

Journal of Crop Science and Biotechnology - Genetic variation and heritability estimates in early generations are important in identifying superior families that can be targeted for genetic...     

U-Pb ages from the neoproterozoic Doushantuo Formation, China

U-Pb zircon dates from volcanic ash beds within the Doushantuo Formation (China) indicate that its deposition occurred between 635 and 551 million years ago. The base records termination of the global-scale Marinoan glaciation and is coeval with similar dated rocks from Namibia, indicating synchronous deglaciation. Carbon isotopic and sequence-stratigraphic data imply that the spectacular animal fossils of the Doushantuo Formation are for the most part younger than 580 million years old. The uppermost Doushantuo Formation contains a pronounced negative carbonate carbon isotopic excursion, which we interpret as a global event at circa 551 million years ago.     

Stabilities of daidzin,glycitin, genistin,and generation of derivatives during heating

The soy isoflavones daidzin, glycitin, and genistin were purified from defatted soy flour using preparative-scale reverse-phase HPLC. The stabilities of the three isoflavones at different heating temperatures were investigated. Daidzin, glycitin, and genistin were lost at a rate of 26, 27, and 27% of their original concentration, respectively, after 3 min at 185 degrees C. At 215 degrees C, decreases of daidzin, glycitin, and genistin were 65, 98, and 74% after 3 min and 91, 99, and 94% after 15 min, respectively. The order of the thermal stabilities, from lowest to highest, was glycitin, genistin, and daidzin. Acetyl daidzin and acetyl genistin, daidzein, glycitein, and genistein were produced during heating at temperatures above 135 degrees C. The rate of binding of an acetyl group to form acetyl daidzin and acetyl genistin from daidzin and genistin was higher than the rate of loss of a glucoside group to form daidzein and genistein. However, acetyl daidzin and acetyl genistin decreased sharply at temperatures above 200 degrees C, while daidzein, glycitein, and genistein were relatively stable over 30 min. The stability of daidzein was higher than that of glycitein or genistein.     

Proteomic analysis and differential expression in protein extracted from chicken with a varying growth rate and water-holding capacity

Chickens from a randomly bred genetic line were segregated into high and low growth rates and high and low water-holding capacities (WHCs). The objective of this study was to identify protein markers associated with slow and fast growth rates and low and high WHCs from water-soluble protein (WSP) and crude myofibrillar protein (CMP) extracts of chicken breast muscle. Proteins were fractionated using two-dimensional electrophoresis, and a total of 22 protein spots were selected, excised, and analyzed by in-gel tryptic digestion and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Proteins expressed in extracts from slow and fast growth rates and low and high WHCs included metabolic enzymes, such as creatine kinase, pyruvate kinase, triosephosphate isomerase, and ubiqitin; housekeeping proteins, such as heat shock protein; contractile proteins, such as myosin heavy chain; actin; and also MHC isoforms and actin isoforms. The mass spectra of 20 protein spots significantly matched (protein score >83; P < 0.05) an online database. In CMP, there were unique proteins that were present only in the fast-growth population: gi|118099530 , gi|20664362 , gi|71895043 , gi|114794125 , gi|297343122 , and gi|71895043 . This information identified protein markers associated with growth rate and water holding capacity. Some of those protein markers could be added to the chicken database.     

Macronutrient (N, P, K) and Redoximorphic Metal (Fe, Mn) Allocation in Leersia oryzoides (Rice Cutgrass) Grown Under Different Flood Regimes

Vegetated drainages are an effective method for removal of pollutants associated with agricultural runoff. Leersia oryzoides, a plant common to agricultural ditches, may be particularly effective in remediation; however, research characterizing responses of L. oryzoides to flooding are limited. Soil reduction resulting from flooding can change availability of nutrients to plants via changes in chemical species (e.g., increasing solubility of Fe). Additionally, plant metabolic stresses resulting from reduced soils can decrease nutrient uptake and translocation. The objective of this study was to characterize belowground and aboveground nutrient allocation of L. oryzoides subjected to various soil moisture regimes. Treatments included: a well-watered and well-drained control; a continuously saturated treatment; a 48-h pulse-flood treatment; and a partially flooded treatment in which water level was maintained at 15 cm below the soil surface and flooded to the soil surface for 48 h once a week. Soil redox potential (Eh, mV) was measured periodically over the course of the 8-week experiment. At experiment termination, concentrations of Kjeldahl nitrogen, phosphorus (P), potassium (K), iron (Fe), and manganese (Mn) were measured in plant tissues. All flooded treatments demonstrated moderately reduced soil conditions (Eh < 350 mV). Plant Kjeldahl nitrogen concentrations demonstrated no treatment effect, whereas P and K concentrations decreased in aboveground portions of the plant. Belowground concentrations of P, Mn, and Fe were significantly higher in flooded plants, likely due to the increased solubility of these nutrients resulting from the reductive decomposition of metal–phosphate complexes in the soil and subsequent precipitation in the rhizosphere. These results indicate that wetland plants may indirectly affect P, Mn, and Fe concentrations in surface waters by altering local trends in soil oxidation–reduction chemistry.     

Sustainable Management of Nutrients in Forage-Based Pasture Soils: Effect of Animal Congregation Sites (5 pp)

Background, Aim and Scope   Grazing animals have a dominant effect on the movement and utilization of nutrients through the soil and plant system, and thus on the fertility of pasture soils. Grazing can accelerate and alter the timing of nutrient transfers, and increase the amount of nutrients cycled from plant to soil. Long periods, position of shade, and water resources for grazing cattle can influence the spatial distribution of soil biochemical properties including soil organic carbon (C), total extractable inorganic nitrogen (TEIN), and Melich 1 extractable total phosphorus (TP). The objective of this study is to test whether cattle congregation sites typical on most Florida ranches, such as mineral feeders, water troughs, and shade areas are more nutrient-rich and may contribute more nutrients to surface and groundwater supply than in other pasture locations under Florida conditions. Materials and Methods: Baseline soil samples around and beneath three congregations sites in established (>10 yr) grazed beef cattle pastures at the United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Subtropical Agricultural Research Station (STARS), Brooksville, FL, were collected in 2003, 2004, and 2005. Results: The levels of soil TEIN and TP were significantly (p ≤ 0.001) affected by the interaction of congregation sites and distance away from the center of the sites. Mineral feeders had the highest concentration of TP followed by shades and water troughs. The concentrations of soil TP decreased almost linearly with distance (x = meter) away from the center of the mineral feeders (-5.24x + 55.10; R2 = 0.92; p ≤ 0.001) and the shades (-6.25x + 57.21; R2 = 0.85; p ≤ 0.001). However, the level of TP around the water troughs (-0.25x + 16.91; R2 = 0.09) does not appear to change significantly with distance, staying close to about 13-18 mg kg-1. The levels of TEIN decreased linearly with distance away from the mineral feeders from the center of the shades. A linear model can describe the relationship between TEIN and distance away from the center of shades: -11.3x + 78.2; R2 = 0.95; p ≤ 0.001. The shaded sites (34.25 ± 1.7 mg kg-1) had higher levels than the mineral feeders (7.22 ± 0.60 mg kg-1) or water troughs (10.06 ± 0.8 mg kg-1) sites. Discussion: The higher soil TP near and around the mineral feeders can be attributed to the presence of phosphorus in the supplemental feeds. The average level of soil TP in the mineral feeders of 34.05 ± 0.44 mg kg-1 was not high enough to be of environmental concern. Losses of soil phosphorus by overland flow are becoming a big concern when the concentrations for soil phosphorus exceeded 150 mg kg-1 in the upper 20 cm of soils. With TN, the shaded sites (34.25 ± 1.7 mg kg-1) had either higher levels than the mineral feeders (7.22 ± 0.60 mg kg-1) or water trough (10.06 ± 0.80 mg kg-1) sites. Higher TEIN content at the shade sites may have been more likely due to frequent urination of animals and lack of vegetation immediately adjacent to shades. The lack of vegetation within and/or near the shades then had no uptake mechanism for removal of inorganic nitrogen, unlike the heavy demand for inorganic nitrogen by bahiagrass in other areas of the pasture. An accumulation of TEIN immediately adjacent to shades could lead to a potential point source that would be susceptible to leaching or gaseous losses to the environment. Conclusions: Early results of the study are suggesting that cattle congregation sites in beef cattle operations in Florida are not nutrient-rich, therefore may not contribute more nutrients to surface and groundwater supply under Florida conditions. Recommendations and Perspectives: Since there is no apparent vertical build up or horizontal movement of TEIN and TP in the landscape, we can then surmise that cattle congregation sites may be considered not a potential source of nutrients at the watershed level, at least on the sand ridge soils in Florida. Further research is continuing, including sampling at cattle congregation sites at other locations in north and south Florida, which started in July 2004.