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Samira Pirmanesh Rouha Kasra Kermanshahi Sara Gharavi Elahe Mobarak Qamsari 《Iranian Biomedical Journal》2022,26(2):153
Background: Lipase enzymes are of great importance in various industries. Currently, extensive efforts have been focused on exploring new lipase producer microorganism as well as genetic and protein engineering of available lipases to improve their functional features. Methods: For screening lipase-producing lactobacilli, isolated strains were inoculated onto tributyrin agar plates. Molecular identification of lipase-producing Lactobacilli was performed by sequencing the 16Sr DNA gene, and a phylogenetic tree was constructed. The LAF_RS05195 gene, encoding lipase protein in L. fermentum isolates, was identified using specific primers, amplified by PCR (918 bp) and cloned into the pET28a (+) vector. The recombinant proteins were expressed 2, 4, 6, and 12 hours after induction with IPTG and assessed using the SDS-PAGE. Enzymatic activity of the purified recombinant protein was measured at 410 nm in the presence of ρ-NPA and ρ-NPP. Results:Among five identified native lipase-producing isolates, one isolate showed 98% similarity with Enterococcus species. The other four isolates indicated 98% similarity to L. fermentum. After purification steps with Ni-NTA column, a single protein band of about 34 kDa was detected on SDS- PAGE gel. The enzymatic activity of purified recombinant protein alongside ρ-NPA and ρ-NPP was measured to be 0.6 U/ml and 0.2 U/ml, respectively. Conclusion:In the present research, a novel lipase/esterase from L. fermentum was cloned and expressed. The novel lipase/esterase has the merit to be further studied due to its substrate specificity. Key Words: Escherichia coli, Gene expression, Lactobacillus, Lipase, Phylogeny 相似文献
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Sousa Larissa Nascimento Batista Samira Pereira Silva Samara Santos da Silva Rômulo Fylipe Sarmento Wlysse Ferreira Mota Rinaldo Aparecido Brasil Arthur Willian Lima Amaku Marcos Feitosa Thais Ferreira Vilela Vinícius Longo Ribeiro 《Veterinary research communications》2022,46(2):507-515
Veterinary Research Communications - This study aimed to describe the transmission of T. gondii in naturally infected goats in the semiarid region of northeastern Brazil, through evaluating the... 相似文献
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Desneux Nicolas Han Peng Mansour Ramzi Arn Judit Brvault Thierry Campos Mateus R. Chailleux Anais Guedes Raul N. C. Karimi Javad Konan Kouassi Arthur J. Lavoir Anne-violette Luna Mara G. Perez-Hedo Meritxell Urbaneja Alberto Verheggen Franois J. Zappal Lucia Abbes Khaled Ali Abid Bayram Yunus Cantor Fernando Cuthbertson Andrew G. S. De Vis Raf Erler Fedai Firake Dnyaneshwar M. Haddi Khalid Hajjar M. Jamal Ismoilov Khasan Jaworski Coline C. Kenis Marc Liu Hao-tian Madadi Hossein Martin Thibaud Mazih Ahmed J. Messelink Gerben Mohamed Samira A. Nofemela Robert S. Oke Abiola Ramos Csar Ricupero Michele Roditakis Emmanouil Shashank Pathour R. Wan Fang-Hao Wang Ming-hui Wang Su Zhang Yi-Bo Biondi Antonio 《Journal of pest science》2022,95(1):17-39
Journal of Pest Science - The South American tomato pinworm, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), has invaded most Afro-Eurasian countries and is threatening worldwide tomato... 相似文献
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Ayelo Pascal M. Mohamed Samira A. Chailleux Anas Yusuf Abdullahi A. Pirk Christian W. W. Deletre Emilie 《Journal of pest science》2022,95(2):633-652
Journal of Pest Science - Semiochemicals such as herbivore-induced plant volatiles (HIPVs) and host chemicals serve as communication signals for parasitoids searching for oviposition sites. The... 相似文献
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- In order to increase their catches, longliners targeting sharks in the Gulf of Gabes, Tunisia, have increased the number of hooks per basket and have included new bait types instead of mackerel (Scomber scombrus). This paper reports the effects of these changes on catch composition, catch rates, size distribution, life‐stage captures, and mortality at haulback.
- Data from 48 and 96 longline sets, carried out during the shark fishing seasons of 2007 and 2008, with two hooks per basket, and 2016 and 2017, with five hooks per basket, respectively, were examined to assess the effect of gear change. Moreover, the effects of bait types were inspected based on 33 fishing sets using whole mackerel, 19 using salema (Sarpa salpa) halves, and 27 using pieces of stingray (Dasyatis spp.), sampled during 2016 and 2017.
- The species composition indicated that longliners expand the vertical distribution of their hooks to operate from the surface to the bottom. The catches with both longline designs were dominated by sandbar shark (Carcharhinus plumbeus). Despite gear changes, the catch rate of C. plumbeus decreased by 42.21%, suggesting population decline. The size composition indicated a shift towards larger specimens, mainly adult females, which could jeopardize the shark population. Moreover, the mortality of the main species at haulback seems to be affected by the design of the longline.
- The bait used, mainly pieces of stingray, significantly increased the catch rate of sandbar shark, which suggests a greater attractiveness of the new bait; however, fish size and mortality rates at haulback were unchanged in response to bait variation.
- The fishery operates in shark nursery grounds, which exposes these fish (principally C. plumbeus) to considerable exploitation pressure. The new fishing practices intensified the pressure and thus the risk of a rapid depletion of populations. To preserve the shark species in the area, the use of new gear and attractive bait should be banned.
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Bechir Saidi Khaled Echwikhi Samira Enajjar Sami Karaa Imed Jribi Mohamed N. Bradai 《水产资源保护:海洋与淡水生态系统》2020,30(6):1172-1181
- This study evaluated the circle hook use as a tool for shark management in the pelagic longline fishery in the Gulf of Gabès.
- The usual J‐hook No. 2 with 10° offset, which has been traditionally used by the fishery, was compared to the 18/0 non‐offset circle hook in an alternating fashion along the main line. In total, 22 experimental longline sets were deployed through the shark fishing seasons of 2016 and 2017 to examine the effects of hook types on the catch composition, the catch rates, the hooking location, and the status at haulback.
- The catch composition differed significantly among hook types. Moreover, an overall increase in catch rates for the main species, the sandbar shark Carcharhinus plumbeus, and the shortfin mako shark Isurus oxyrinchus, was observed when using circle hooks.
- There was no size‐selective effect of circle hooks for the common species. The circle hooks were not effective at reducing at‐haulback mortality of sandbar shark. Conversely, shortfin mako and smooth‐hound shark Mustelus mustelus showed significantly lower relative mortality at haulback with circle hooks than with J‐hooks. Furthermore, circle hooks were more frequently hooked externally than the J‐hooks for the three shark species.
- Results demonstrated that the use of 18/0 non‐offset circle hooks in the pelagic shark longline fishery can reduce mortality at haulback for some species without any benefit for the dominant species, the sandbar shark.
- Overall, it is difficult to promote the adoption of the use of circle hooks as a management measure in this specialized fishery. Management measures focusing on fishing effort controls, fishing closures in critical habitats, and size limits could have significant benefits for the conservation of shark species and may help to improve the sustainability of the shark fishery in the Gulf of Gabès.
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Morrow EM Yoo SY Flavell SW Kim TK Lin Y Hill RS Mukaddes NM Balkhy S Gascon G Hashmi A Al-Saad S Ware J Joseph RM Greenblatt R Gleason D Ertelt JA Apse KA Bodell A Partlow JN Barry B Yao H Markianos K Ferland RJ Greenberg ME Walsh CA 《Science (New York, N.Y.)》2008,321(5886):218-223
To find inherited causes of autism-spectrum disorders, we studied families in which parents share ancestors, enhancing the role of inherited factors. We mapped several loci, some containing large, inherited, homozygous deletions that are likely mutations. The largest deletions implicated genes, including PCDH10 (protocadherin 10) and DIA1 (deleted in autism1, or c3orf58), whose level of expression changes in response to neuronal activity, a marker of genes involved in synaptic changes that underlie learning. A subset of genes, including NHE9 (Na+/H+ exchanger 9), showed additional potential mutations in patients with unrelated parents. Our findings highlight the utility of "homozygosity mapping" in heterogeneous disorders like autism but also suggest that defective regulation of gene expression after neural activity may be a mechanism common to seemingly diverse autism mutations. 相似文献
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Roufik S Gauthier SF Dufour E Turgeon SL 《Journal of agricultural and food chemistry》2006,54(14):4962-4969
Front-face fluorescence spectroscopy was used for the first time to study the interactions between bovine beta-lactoglobulin variant A (beta-Lg A) and various beta-Lg-derived bioactive peptides. Fluorescence spectra were recorded for beta-Lg A-peptide mixtures at 25 degrees C and pH 6.8 with an excitation wavelength of 290 nm to characterize the molecular environment of tryptophan (Trp) residues present in the protein but absent in the peptides. Spectra remained unchanged following addition of peptides beta-Lg f92-100 and beta-Lg f125-135, while Phe-Phe interaction between beta-Lg f69-83 molecules interfered with analysis. Addition of beta-Lg f102-105 produced a blue shift (3 nm) and a significant increase in fluorescence intensity, while addition of beta-Lg f142-148 also caused a significant increase in fluorescence intensity but accompanied by a red shift (3 nm). These results indicate that the polarity of the Trp environment in the beta-Lg A structure may be modified differently depending on the peptide added. 相似文献