村支书工作倦怠感研究

研究就村支书工作倦怠源、工作倦怠感以及心理上、生理上、行为上产生的一系列不良反应进行探索,并且给出改善工作倦怠感的建议和意见。     

表达Omp1和Omp2的猪大肠埃希菌融合菌株R2的构建

为了构建猪大肠埃希菌融合菌株,在药敏试验和MIC试验的基础上,对猪大肠埃希菌HY2株(O101,Omp1)和GXA1株(O107,Omp2)进行交叉耐药培育.以HY2(O101,Omp1,Amir,Czls)和GXA1(O107,Omp2,Czlr,Amis)为亲本菌株,采用溶菌酶-EDTA法制备两亲本菌株原生质体,通过聚乙二醇(PEG)助融,进行原生质体融合,得到3株双耐药融合菌株.结果表明,猪大肠埃希菌原生质体制备和再生的最适条件是:溶菌酶浓度为200 U/mL,作用时间为25 min.筛选得到3株融合菌株R1、R2、R3,均可凝集两亲本的O抗原,其中,R2凝集性更好更稳定.进一步结合SDS-PAGE分析,显示融合菌株R2能较好的表达两亲本菌株形状,经多次传代仍能够稳定遗传.     

干旱地区膜下滴灌技术甜瓜种植模式试验研究

本文通过田间试验,探索干旱地区甜瓜由传统起垄滴灌种植变为平地滴灌种植的可行性。同时,探讨与滴灌相适宜的植株密度以及经济的滴灌带布置方式,以提高甜瓜种植的经济效益。本文可为类似地区推广平地滴灌技术提供借鉴。     

Cloning of Inhibin-α Gene and its mRNA Expression Pattern in the Ovary of Congjiang Xiang Pig

To reveal the relationship between inhibin-α(INHA) gene and the reproductive traits of Congjiang Xiang pig, INHA gene was cloned and sequenced taking the genomic DNA of Congjiang Xiang pigs as templates by polymerase chain reaction(PCR) method.The polymorphisms of INHA gene were tested in Congjiang Xiang pig populations with high-litter size and low-litter size using allele-specific PCR(AS-PCR) method.The expression profile of INHA gene in ovaries was detected from Congjiang Xiang pigs with high-litter yiled or low-litter yiled by Real-time PCR method.The complete coding region of INHA gene was 1095 bp in length, which coded for 364 amino acid residues.Compared with the known sequence, two candidate sites, G359A and A373G, were found out from exon 2 region of INHA gene in Congjiang Xiang pig.After investigation for the two sites in a large population, the frequency of alleles between two populations was not significant and without obvious relativity with the litter yiled of Congjiang Xiang pig.However, the INHA mRNA level in the ovary of Congjiang Xiang pig with high-litter yiled was higher than that with low-litter yiled.It suggested that INHA gene was much conserved, INHA gene expression level might be concerned for the regulation of ovary growth and follicle development in Congjiang Xiang pig breed.     

鸭IFIT5基因(544G>A)多态性与部分免疫指标的关联分析

探索IFIT5基因作为抗性性状的候选标记的可能性,为家禽抗病育种提供一些参考依据。以樱桃谷北京鸭、苏牧麻鸭(樱桃谷鸭×金定鸭)、金定鸭、白羽番鸭为试验材料,利用DNA直接测序技术扫描IFIT5基因单核苷酸多态性(SNP),并分析其与部分免疫指标的关联性。结果表明:仅在IFIT5基因外显子2上检测到一个错义突变(G544A),产生2种等位基因,2种基因型(GG、GA),未发现有AA基因型。经χ2检验,4个鸭群体只有金定鸭和苏牧麻鸭处于Hardy-Weinberg平衡状态。关联分析结果显示:樱桃谷鸭体内H5含量GG型显著高于GA型(P0.05),而Ig M含量GG型显著低于GA型(P0.05),其余免疫指标均无显著差异(P0.05);苏牧麻鸭体内H5含量GG型极显著高于GA型(P0.01),其余免疫指标均无显著差异(P0.05);金定鸭不同基因型各免疫指标均无显著差异(P0.05)。白羽番鸭在+544位点均为GG纯合基因型。由此可以看出,IFIT5(544GA)可作为樱桃谷鸭体内H5含量的候选分子标记。     

珍稀濒危植物膝柄木种子催芽研究

以采自越南广宁省海河县的膝柄木种子为试材,研究了不同药剂和浓度对种子发芽率和芽苗主根生长的影响。结果表明:与水浸相比,艾比蒂生根剂1号和双吉尔氨基酸肥料6号0.5~15.0mg/L浸种1h,对膝柄木种子发芽均没有促进作用,可见水浸即可有效促进膝柄木种子发芽;艾比蒂生根剂1号0.5mg/L和双吉尔氨基酸肥料6号0.5~10.0mg/L对膝柄木芽苗主根生长有很好的促进作用。     

“莱牧一号”牧草栽培平菇的关键技术研究

随着我国食用菌产业的发展壮大,栽培料需求持续攀升,传统的木屑、棉籽壳等栽培料已经不能满足产业的持续发展。"莱牧一号"(Pennisetum sp.)牧草为莱索托引进的新品种,生物量大,可作为食用菌栽培的原料。现主要介绍"莱牧一号"牧草栽培平菇的技术措施,以期为平菇栽培提供新原料,促进平菇产业的可持续发展。     

蒜和葱上二斑叶螨的发生为害及其抗药性监测

二斑叶螨寄主植物范围极广, 但其对百合科蔬菜造成危害的研究报道极少?本研究采集了来自北京海淀百合科蔬菜蒜和葱上的2个二斑叶螨种群, 生物测定结果显示, 2个种群对阿维菌素呈现出极高水平抗性, 对哒螨灵为中等以上抗性(LC50>5 000 mg/L), 对新型药剂联苯肼酯?虫螨腈和腈吡螨酯也表现出中等到高水平抗性?抗性基因突变频率检测发现, 与阿维菌素抗性相关基因G314D和G326E位点以及和哒螨灵抗性相关基因H92R位点的突变频率均高达100%, 为纯合抗性种群?因此, 本研究所测试的2个二斑叶螨种群对阿维菌素和哒螨灵单剂的敏感性极低, 需慎重选用这2种药剂, 而3种新型杀螨剂联苯肼酯?虫螨腈和腈吡螨酯在田间需轮换使用?     

Measurement and Analysis of Prestressed Concrete Flat Beam- Waffle Slab System During Prestressing

During the pre - stressing of the unbonded prestressed concrete wide flat beam - waffle slab system, the displacements and strains of slab and the prestressed concrete beams were site - measured and the results were analyzed. The prestressing construction was simulated by finite element program. The prestressing efficiency of the unbonded prestressed concrete wide flat beam - waffle slab system was discussed. It indicates that the prestressing efficiency was affected by the in -plane stiffness of slab.     

Biocompatibility of Acellular Porcine Dermis

In vitro cultured vascular endothelial cells (VEC) and mouse 3T3 fibroblasts (3T3) on the acellular dermal matrix , which were made of porcine skins. We made the cell proliferation test with MTT assay and the histological observation after cells were seeded on the acelluar dermis for 1 week with histological section. The cell growth curves showed VEC and 3T3 grew much rapidly on the acelluar dermis.The histological observation revealed VEC had formed a monolayer, some places even had formed 2 to 3 layer. The results suggest that the acelluar porcine dermal matrix have good biocompatibility , it will be widely applied.