台湾青枣不同品种花粉萌发和生活力测定

以5个台湾青枣品种盛花期花粉为试材，应用正交试验测定不同品种花粉萌发率及适宜的萌发条件，并用4种不同方法测定花粉生活力。结果表明：（1）不同品种的花粉萌发率存在显著差异，其中大世界的花粉萌发率最高。（2）硼酸浓度、培养温度等因素对花粉萌发率有极显著影响。最适培养基为含琼脂1％、蔗糖15％、硼酸0．02％的组合，适宜温度为30—32℃。（3）花粉生活力的测定中，离体萌发法是最佳选择，I—KI染色法、醋酸洋红染色法和TTC染色法不适宜用作台湾青枣花粉生活力的测定。     

中草药添加剂提高密蜂春繁效果的研究

在早春繁殖期,以中草药作为饲料添加剂,观察蜜蜂群势变化和蜂群健康情况.结果表明,试验组A和对照组CK的蜂群繁殖效果差异极显著,试验组蜂群健康、活动正常,蜂群繁殖率提高了12.1%.     

高寒地区不同建植期人工草地群落垂直结构和生产力变化的研究

对高寒地区不同建植期人工草地群落垂直结构和生产力的变化研究结果表明：（1）随着人工草地建植时间的延长,人工草地群落垂穗披碱草高度异质性逐渐减小.（2） 不同建植期（1999、1998、1997年建植）人工草地垂穗披碱草和群落平均地上生物量均主要分布在0 ～20cm冠层中,约占平均地上生物量的61.03%、90.64%、84.55%和67.47%、92.54%、86.08%.（3） 不同建植期 （1999、1998、1997年建植）人工草地群落平均地下生物量均主要分布在0～10cm土层中,约占地下总生物量的85.97%、81.73%和78.47%.（4） 建植的人工草地如果其组分单一,即物种的丰富度很低时,均匀度增加,物种对环境资源的竞争力和利用率提高,导致土壤资源库中某些营养成分缺乏,草地群落初级生产力因此而降低。     

Role of p38MAPK in production of pro-inflammatory cytokines in Kupffer cells from severe acute pancreatitis rats

AIM:To investigate the role of p38 mitogen-activated protein kinase (p38MAPK) signaling pathway in the Kupffer cells (KCs) production of pro-inflammatory cytokines, tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β), in severe acute pancreatitis (SAP) rats.METHODS:Sprague-Dwaley rats were randomized into three groups:①sham operation rats, ②SAP rats, ③SAP rats given the p38 MAPK inhibitor CNI-1493(10 mg/kg, iv). The SAP model was induced by the bili-pancreatic duct infusion with 5% sterile soduim taurocholate solution. Rats from each group were killed at 12 h after sham operation or SAP and Kupffer cells (KCs) were isolated. The mRNA expressions of TNF-α and IL-1β (by quantitative real-time RT-PCR) and p38 MAPK activity (by Western blot analysis) in KCs were examined. The levels of TNF-α and IL-1β in plasma were determined by ELISA.RESULTS:There was a significant acvitation of p38 MAPK in KCs harvested from SAP rats than those from sham operation rats. SAP also promoted the mRNA expressions of TNF-α and IL-1β in KCs and the plasma levels of TNF-α and IL-1β. These events were significantly inhibited by treatment with CNI-1493.CONCLUSIONS:p38 MAPK activation is one important aspect of the signaling events that may mediate the KCs production of pro-inflammatory cytokines, TNF-α and IL-1β, in SAP rats. The inhibition of the p38 MAPK may be a potential target in the prevention and treatment of SAP.     

The expression of IFN-γ and IL-4 on T lymphocytes that infiltrate in nasal polyps

AIM:To investigate the expression of Th1-typed cytokine IFN-γ and Th2-typed cytokine IL-4 on T lymphocytes that infiltrate in nasal polyps for searching the pathogenesis of nasal polyps. METHODS:Nasal polyps tissue samples and peripheral blood were obtained from 21 patients. Normal human inferior turbinate mucosa and peripheral blood were obtained as well. Flow cytometry was adopted to detect the expression of IFN-γ and IL-4 of T lymphocytes. RESULTS: Th cytokines were rarely detected in inferior turbinate from normal human. Nasal polyps tissue consisted of abundant T lymphocytes. The expression of IL-4 and IFN-γ increased in peripheral blood from patients compared with normal human (P<0.05). The expression of IL-4 increased but the expression of IFN-γ decreased in nasal polyps compared with that of peripheral blood from the same patient (P＜0.05). CONCLUSION:There were generous of T lymphocytes infiltrating in nasal polyps. There was abnormal immune status in the local nasal mucosa from the patients, and the predomination of Th cytokine secretion changed compared with peripheral blood from the same patients, which resulted in the change of microenvironment of nasal mucosa and possibly close related to the formation of nasal polyps.     

中国斜纹夜蛾寄生蜂名录

根据浙江大学应用昆虫学研究所收藏的寄生蜂标本和国内外报道，对我国寄生斜纹夜蛾的原寄生蜂和重寄生蜂进行系统整理研究。我出我国斜纹夜蛾寄生蜂40种，其中包括原寄生蜂29种，重寄生蜂11种，同时还以附录的形式列出了我国已报道过但有存疑的寄生蜂7种及国外已有报道但在我国还未采集到标本的寄生蜂61种。     

农作物病虫预报可视化发展与应用前景

农作物病虫预报可视化具有形象生动、信息传递快捷、覆盖面广等优点,是更好发挥预报作用的最佳方式。大范围实施这种预报发布形式的条件,目前已基本成熟,经过努力,必然会在不长的时间内取得长足进展。     

电流处理抑制甘薯丛枝病症状及提高茎尖培养成苗率

甘薯丛枝病在我国东南沿海主薯区流行多年,发病率高,是影响我国甘薯产量和品质的一个主要限制因子。其典型症状是植株叶小、褪绿、矮缩和侧枝丛生。甘薯丛枝病病原物是一种类菌质体(Mycoplasma-like organism,MLO),现在国际上统称植原体(Phytoplasma)。     

羊肚菌研究进展

综述了近年来羊肚菌营养成分、有效成分，培养技术，以及细胞学、生物化学和分子生物学等方面的研究进展。     

Effect of Jiawei Sini Decoction on glucocorticoid receptor in thymocytes of chronic psychological stress rats

AIM: To observe the effect of Jiawei Sini Decoction (JWSND) on glucocorticoid receptor (GCR) in thymocytes of chronic psychological stress rats. METHODS: The rats were randomly divided into 4 groups, control group (C), model group (M), group treated by JWSND C₁, group treated by ginsenosides C₂. The number of thymocyte GCR sites and the GCR nuclear thanslocation rate were detected by radioimmunoassay. RESULTS: Compared with the control group, in the model group, the thymocyte weight index lowered significantly ( P<0.05 ), and the GCR nuclear thanslocation rate was increased significantly ( P<0.01 ), but the number of thymocyte GCR sites was unchanged. Compared with the model group, thymus gland weight indexes of C₁ and C₂ were increased significantly ( P<0.05 ), while the GCR nuclear thanslocation rate lowered significantly ( P<0.01 ). Moreover, no significant difference was found in all indexes between C₁ and control group. CONCLUSION: The inhibitory effect of glucocorticoid on the thymus could be significantly reversed by JWSND via suppressing the thanslocation of GCR from cytoplasm to nucleus in chronic psychological stress rats.