Effect of FAK-related non-kinase on apoptosis in hepatic stellate cells

AIM: To evaluate the inhibitory effect of FRNK on the phosphorylation of FAK and apoptosis in hepatic stellate cells (HSCs). METHODS: After stimulated with fibronectin, HSCs was transfected with FRNK plasmid by cationic liposome method. The apoptosis of FRNK-induced HSCs was examined by Annexin-V/propidium iodide double-labeled flow cytometry (FCM), gel electrophoresis and transmission electron microscope. The protein levels of FRNK, FAK and p-FAK (Tyr397) in HSCs were assayed by Western blotting, and RT-PCR was used to detect the expression of mRNA. RESULTS: The expression of FRNK was enhanced and the phosphorylation of FAK was inhibited after FRNK was transiently transfected into HSCs in vitro. The apoptotic rate in HSCs exposed to FRNK plasmid for 48 h was higher than that in the non-FRNK plasmid group ［(25.37±1.92) % vs (9.28±1.05) %, P<0.01］, and accompanied by a significant increase in caspase-3 activity both in the protein and in the mRNA level ［(264.17±12.60 vs 185.82±9.69), P<0.01; (4.19±0.48 vs 1.07±0.27), P<0.01］. CONCLUSION: In HSCs, the expression of FRNK is enhanced and the phosphorylation of FAK is inhibited after FRNK transfection. FRNK induces the HSCs apoptosis.     

Expression of tissue factor,tissue factor pathway inhibitor in the atherosclerotic plaque on femoral artery of New Zealand rabbits

AIM: To observe the regulatory changes of tissue factor (TF),tissue factor pathway inhibitor-1 (TFPI-1) and tissue factor pathway inhibitor-2 (TFPI-2) associated with the formation of atherosclerotic plaque on femoral artery of New Zealand rabbits.METHODS: New Zealand rabbits were fed with high cholesterol diet and received femoral artery balloon injury to establish moderated atherosclerotic plaque animal model.These rabbits were classified into 8 weeks group,10 weeks group and 12 weeks group.The expressions of factor TF,TFPI-1 and TFPI-2 in femoral artery were detected by immunohistochemical methods at 8th,10th and 12th week.The positive areas of the slice were analyzed by semi-quantification system.Fluorescence quantification PCR (FQ-PCR) was used to detect the copies of mRNA of TF,TFPI-1 and TFPI-2 in each group.RESULTS: The expressions of TF,TFPI-1 and TFPI-2 determined by immunohistochemical methods were increased from 8th,10th to 12th week,respectively.In each group,the quantity of mRNA of TF and TFPI-1 at the 8th,10th and 12th week was soared,respectively.In each group,the quantity of mRNA of TFPI-2 did not change in early phase,but decreased at 12th week.CONCLUSION: In the process of the atherosclerotic plaque formation in rabbit femoral artery model,the expressions of TFPI-1 and TFPI-2 proteins are increased.However,the expression of TPFI-1 or TFPI-2 could not stave off the development of plaque formation.     

湄江翠片加工中香气与色泽的相关性初探

本文详细介绍了目前湄江翠片茶各种工艺组合，并根据制茶原理，分析了制茶因子对湄江翠片的品质影响，通过分析对比，找出了湄江翠片比较合理的工艺组合。     

浅谈坡面覆盖材料

介绍了国内外坡面覆盖材料的发展情况,对常见材料进行分类和介绍,定义了一些专业名词,并展望我国边坡覆盖材料的发展前景。     

鸭肿头出血症病毒强毒的致病性及感染组织细胞的超微病理变化

将鸭肿头出血症病毒（DSHDV）强毒株以不同途径接种10日龄鸭胚，研究了病毒在鸭胚中的繁殖规律，并用透射电镜观察了感染鸭胚各组织器官的超微结构变化，同时用DSHDV人工感染不同日龄雏鸭，比较了不同感染途径对雏鸭的致病性和对不同日龄雏鸭的致病性。结果显示，尿囊腔和卵黄囊2种途径均能使病毒在鸭胚上繁殖并传代，对鸭胚的半数致死量分别为10^-7.24／0．2mL和10^-6.4／0．2mL。病毒感染鸭胚后，电镜下可观察到肝和尿囊膜中的病毒粒子，病毒粒子呈球形或椭圆形，大小为60～80nm，无囊膜；病毒在细胞浆内复制，可形成特征性包涵体；病毒侵害的主要靶器官为尿囊膜与肝，细胞器的变化主要表现为粗面内质网扩张以及线粒体肿胀和嵴断裂、消失。病毒经肌肉注射、口服和滴鼻均能使28日龄鸭感染发病，致死率分别为86％、89％和97％。研究表明，DSHDV能很好地在鸭胚上生长并致鸭胚各组织器官超微结构发生变化，不同感染途径均能使雏鸭感染发病且致病性强。     

一种用C18柱纯化低浓度微量寡核苷酸的新方法

通过比较4种不同的回收方法和C18硅胶4种不同的用量对回收的影响,提出了回收低浓度微量寡核苷酸的最佳方案:分别用1 mL乙腈、2 mL灭菌双蒸水和1 mL 10 mmol/L乙酸铵平衡装填10 mg硅胶的C18 Sep-Pak反相层析柱;再将寡核苷酸溶液通过C18柱;然后用1 mL 25 mmol/L碳酸氢铵洗脱C18柱,并以3000 r/m in离心2 m in;最后用1 mL 30%乙腈洗脱C18柱,将收集的溶液离心冻干后即得到纯化的寡核苷酸。该方法的回收率可达53.7%±3.0%。     

丙烯酸松香的溴化及产物的阻燃性研究

以丙烯酸松香与液溴加成,合成了溴化丙烯酸松香;研究了反应温度、原料的物质的量比、反应时间等因素对反应过程及产物性能的影响.用FT-IR、1 HNMR和热重分析(TG)等对溴化丙烯酸松香进行了表征.结果表明,当反应温度为-2 ～ 4℃、反应时间5 ～ 6h、液溴与丙烯酸松香的物质的量比值约为1.2时,收率可达93%.溴化丙烯酸松香不仅具有较好的阻燃性,同时还拓宽了松香的应用范围,提高了其附加值.