Guanine-cytosine contents of the host and symbiont cDNA in a symbiotic coral

ABSTRACT:   Hermatypic (reef-building) corals harbor dinoflagellate endo-symbionts Symbiodinium spp. In studying gene expression in such symbiotic corals, problems arise regarding how to distinguish the coral and symbiont mRNA, and how to estimate their fractions in the mRNA population of the holobiont (symbiotic complex of the coral and Symbiodinium cells). In this study, these issues were addressed using juveniles of hermatypic coral Acropora tenuis in symbiosis with Symbiodinium cells of strain PL-TS-1. First, the guanine-cytosine (GC) contents were determined in expressed sequence tags (EST) from PL-TS-1 cells cultured in vitro and symbiont-free larvae of A. tenuis , and their average GC contents were found to be significantly different. The average GC content of the EST from the holobiont was much closer to that of A. tenuis larvae, suggesting that the majority (>90%) of mRNA isolated from the holobiont originated in the host. In protein-coding sequences, little overlap was observed between the GC-content distributions of PL-TS-1 cells and A. tenuis larvae. All of the coding sequences ( n  = 59) found in the A. tenuis EST had GC contents below 0.5, whereas the GC content exceeded 0.5 in the majority (43/44) of coding sequences from the nuclear genome of PL-TS-1 cells.     

Bioaccumulation of cadmium and its biochemical effect on selected tissues of the catfish (<Emphasis Type="Italic">Clarias gariepinus</Emphasis>)

The present study examines the pattern of accumulation of cadmium (Cd) and its biochemical effects on selected tissues of a variety of African catfish (Clarias gariepinus), after exposure to various doses of Cd. The results obtained indicate that at the end of 21 days of exposure, the total tissue organ cadmium concentration followed the pattern kidney > gill > liver > muscle for each of the exposure concentrations. The levels of Cd in these organs were higher than those in ambient water. Moreover, while the rate of uptake of Cd increased with time in the kidney, liver and muscle, it decreased in the gill. Superoxide dismutase (SOD) was significantly elevated only in the kidney of catfish treated with 0.2 and 0.4 ppm of Cd for 7 days compared with the control. Conversely, gill SOD was significantly decreased in the same concentrations of Cd-treated catfish relative to the control. Statistically similar levels of SOD were observed in the liver, brain and muscle with all the treatments after the same duration of treatment. In the fish exposed for 21 days, SOD activity was significantly decreased in the kidney with a corresponding increase in lipid peroxidation (LPO), but it manifested only with the 0.2- and 0.4-ppm Cd treatment relative to the control. In the liver, however, Cd exposure significantly increased SOD in the 0.2- and 0.4-ppm treatments of the same duration. In conclusion, the present study indicates that the accumulation of Cd and its effect on SOD and LPO in C. gariepinus is dependent on concentration, tissue and time     

轮回选择法改良甘蓝型油菜核不育系恢复系群体品质(英文)

[目的]用轮回选择法对甘蓝型油菜核不育两系恢复系的品质性状进行改良。[方法]利用显性核不育系作为轮回选择的异交媒介,导入13份常规材料的优良基因,结合自交选择改良群体的品质性状,优选恢复系材料。并对改良群体的遗传多样性、遗传增益、脂肪酸链长度及不饱和度进行分析。[结果]轮回选择方法有效改良群体材料的遗传多样性及遗传增益。通过轮回选择筛选获得9份含油量超过50%和14份蛋白含量超过30%的材料。部分材料与成熟的不育系杂交进行组合比较实验,获得11个含油量显著高于对照的组合。[结论]轮回选择是改良甘蓝型油菜核不育系恢复系群体品质的一个有效的育种方法。     

学籍微机管理系统的研制

本系统根据学生学籍管理的目标和任务,利用FOXBASE 2.1数据库管理系统编制而成。该系统具有对数据的录入和维护(修改、删除及追加)、统计与查询、打印输出等功能;对学生基本情况、在校成绩、综合测评、升留级情况及毕业、肄业等项目进行统计管理。     

Study on the Acute Toxicity of Rare Earth Yttrium to Earthworms under the Stress of Leaching Agent Ammonium Sulfate

[目的]考察浸矿剂硫酸铵（NH4）2SO4胁迫下稀土离子钇的毒性研究。[方法]选择蚯蚓作为土壤生态环境污染指示生物,采用滤纸接触法研究硫酸铵胁迫下稀土钇（ Y）对蚯蚓的急性毒性效应。[结果]①稀土钇单一染毒,蚯蚓的48 h 半致死率浓度为 LC50=213.41 mg/L、24 h半致死率浓度为 LC50=322.63 mg/L。②硫酸铵单一染毒下,蚯蚓的48 h半致死率浓度为 LC50=13.89 g/L、24 h半致死率浓度为LC50=15.05 g/L。③低浓度10 g/L的硫酸铵与稀土钇复合染毒,蚯蚓的48 h半致死率浓度LC50=198.65 g/L、24 h 半致死率浓度 LC50=399.85 g/L;中浓度14 g/L的硫酸铵与稀土钇复合染毒,蚯蚓的48 h半致死率浓度 LC50=167.3 mg/L、24 h半致死率浓度 LC50=256.73 mg/L;高浓度20 g/L的硫酸铵与稀土钇复合染毒,蚯蚓的48 h半致死率浓度 LC50=31.03 mg/L、24 h的LC50=127.65 mg/L。[结论]低浓度的硫酸铵降低稀土钇对蚯蚓的毒性,产生一定的拮抗作用。中浓度的硫酸铵增加稀土钇对蚯蚓的毒性,产生较明显的协同作用。高浓度硫酸铵显著增加了稀土钇对蚯蚓的毒性。稀土钇染毒下蚯蚓死体更易断裂,而活体对针刺反应相对不灵敏。     

对母猪急性风湿病的治疗对比试验研究

选择96头母猪急性风湿病病例,随机分成3组,分别采用不同的治疗方案进行治疗,以对比不同的治疗方案所产生的治疗效果。结果表明:水杨酸钠、乌洛托品、安钠咖混合使用方案2,效果最好(有效率达到100%,治愈率97%);氢化泼尼松方案1效果次之(有效率是68%,治愈率62%);维生素C、葡萄糖氯化钠混合使用,方案3效果最差(有效率是50%,治愈率31%);方案2的有效率高出方案132%(P<0.01),高出方案3 50%(P<0.01);方案2的治愈率高出方案1 35%(P<0.01),高出方案3 66%(P<0.01);方案1与方案3的有效率差异不显著(P<0.05),治愈率差异显著(P<0.05)。     

浅议乐都县种子工作现状及对策

1现状多年来,乐都县坚持四自一辅四化一供的种子工作方针,大力繁殖推广优良新品种,为乐都县的农业增产丰收发挥了重要作用.但随着市场经济体制的不断完善,<种子法>的颁布实施,以及农业经济结构的调整,乐都县种子产业与农业发展有很多不适应性,暴露出很多问题.     

农村居民的人口学特征与有效实施农村环境教育的关系探讨——以江西省为例

农村环境教育是事关农村环境保护大局的基础性工作,是当前建设社会主义新农村的必然要求。农村环境教育应该面向所有的农村居民,但在具体实施农村环境教育时,要依据人口学特征,区分不同的目标人群,并据此确定不同教育内容和方式。     

Breaking new ground in food regime theory: corporate environmentalism,ecological feedbacks and the ‘food from somewhere’ regime?

Early food regimes literature tended to concentrate on the global scale analysis of implicitly negative trends in global food relations. In recent years, early food regimes authors like Harriet Friedmann and Philip McMichael have begun to consider the sites of resistance, difference and opportunity that have been emerging around, and into contestation with, new food regime relations. This paper examines the emerging global-scale governance mechanism of environmental food auditing—particularly those being promoted by supermarkets and other large food retailers—as an important new dynamic in our understanding of the politics and potentials of food regimes. Commencing with an examination of Friedmann’s corporate environmental food regime, two key dynamics are identified as being pivotal in the rise and decline of global-scale regimes: securing social legitimacy for food relations and the importance of ecological dynamics in global food relations. By extending McMichael’s notion of ‘Food from Nowhere’ versus ‘Food from Somewhere’, the paper interrogates the emergence of a cluster of relations that comprise ‘Food from Somewhere’ and examines whether this cluster of relations has the potential to change some of the constituent ecological dynamics of food regimes. These ecological dynamics have historically been problematic, amply demonstrating Marx’s metabolic rift as the early food regimes solidified relationships between ‘ecologies at a distance’. By using socio-ecological resilience theory, ‘Food from Somewhere’ is characterized as having denser ecological feedbacks and a more complex information flow in comparison to the invisibility and distanciation characterizing earlier regimes as well as contemporary ‘Food from Nowhere’. The conclusion of this article is that while ‘Food from Somewhere’ does provide one site of opportunity for changing some key food relations and ecologies, the social legitimacy of this new form of food relations does rely on the ongoing existence of the opposite, more regressive, pole of world food relations. The key question for resolving this tension appears to be whether new food relations can open up spaces for future, more ecologically connected, global-scale food relations.     

Gender discrimination and its impact on income,productivity, and technical efficiency: evidence from Benin

This paper examines the occurrence and impact of gender discrimination in access to production resources on the income, productivity, and technical efficiency of farmers. Through an empirical investigation of farmers from Koussin-Lélé, a semi-collective irrigated rice scheme in central Benin, we find that female rice farmers are particularly discriminated against with regard to scheme membership and access to land and equipment, resulting in significant negative impacts on their productivity and income. Although women have lower productivity, they are as technically efficient as men. The findings suggest that there is considerable scope for improving the productivity of women through increasing their access to production resources.