欧美杂交杨Pnd-LRR3基因克隆及其抗锈菌侵染表达

为深入探究欧美杂交杨(Populus nigra×P.deltoides)感病的分子机理,以及抗病基因在发病过程中所起的作用,克隆了欧美杂交杨LRR3蛋白编码基因Pnd-LRR3。对Pnd-LRR3基因进行了生物信息学分析,并对其在抗锈菌过程中的功能进行了研究。Q-PCR结果显示,强致病性的落叶松-杨栅锈菌(Melampsora larici-populina)E4菌株接种6 h,Pnd-LRR3基因表达量上调增幅较大,168 h为显著下调。说明Pnd-LRR3基因在E4侵染过程中起到一定抗性作用,但最终无法阻止锈菌的侵染。     

紫薇栽培管理技术

指出了紫薇作为城乡绿化的重要树种，适宜于庭院、园路两侧、池畔河边栽植。结合栽培管理实践，系统地总结了紫薇树的育苗、修剪和病虫害防治技术。     

Cloning and Expression of Bile Salt Hydrolase Gene from Lactobacillus plantarum M1-UVS29

We cloned and expressed bile salt hydrolase gene of Lactobacillus plantarum M1-UVS29 in Lactococcus lactis NZ9000 successfully. Gene-specific primers for amplification of L. plantarum bsh were designed by using sequence which availabled from Gen Bank. The production of PCR amplicon was confirmed by sequencing and cloned into pMD18-T vector, and then recombined into expression vector p NZ8148 and yielding vector p NZ8148-BSH. p NZ8148-BSH was transferred into Lactococcus lactis NZ9000. Sequencing indicated that the cloned bsh fragment contained 995 nucleotides, and shared 99.3% sequence homology with bsh gene from L. plantarum MBUL10. Cloned bsh fragment was successfully transduced into NICE expression system and confirmed by PCR and restriction digest. Recombinant BSH protein was analyzed by SDS-PAGE. The molecular weight of BSH protein was approximately 37 ku. Activity of the expressed protein was 0.77 μmol · min-1. The successfully expressed proteins by genetic engineering technology made the function of lactic acid bacteria be abundant and laid the foundation for further researches into cholesterol-lowering lactic acid bacterium food and probiotics.     

基于模拟退火算法优化给水管线造价公式研究

给水管线造价公式的精确性对工程经济分析与给水管网优化的科学性和经济性有重大影响。通过五个地区的铸铁管综合单价数据,采用最小二乘法对各组数据进行多项式一至十次的拟合,确定了给水管线造价公式的最佳拟合次数为三次;进一步采用模拟退火算法对拟合的多项式参数进行优化计算,求解给水管线造价公式。结果表明:凭借模拟退火算法随机全局搜索模式以及不受函数性质影响的优势,能够克服传统算法难以求解多阶导数的困难,提高公式拟合精度。     

高寒地区蛹虫草大米培养基的优化

以引进西藏的6株蛹虫草为试材,对比研究了其菌丝生长速率、出草产出及长势,筛选优良的蛹虫草菌株,并通过正交实验筛选对蛹虫草大米培养基营养成分的最优水平组合。结果表明:蛹虫草"TAAAS1"的菌丝生长速率虽不是最快的,但出草整齐均匀,出草产出高于其它菌株,表明"TAAAS1"菌株是适宜在高寒地区栽培生产的好品种;蛹虫草大米培养基的最优水平组合为蛹粉4g/L,葡萄糖20g/L,硫酸镁2g/L,磷酸二氢钾1.5g/L。     

兰州引黄灌区玉米田杂草群落及生态位研究

对兰州引黄灌区玉米田杂草进行了研究,初步了解了其杂草种类和杂草种群的消长动态。主要杂草有打碗花、田旋花、藜、刺儿菜、荠菜、稗草、马齿苋、反枝苋、狗尾草。主要杂草的干重为藜、反枝苋、稗草分别列第1、第2和第3位。生态位的研究结果表明,藜的时间、水平、垂直生态位宽度值最高,分别为0.952 6,0.971 8,0.937 9,其危害最大;其次为反枝苋和稗草。藜、反枝苋和稗草为优势种群,与玉米存在时间、水平、垂直空间的竞争激烈,并且这3种杂草的生态位重叠值也最大,因此在使用一种除草剂时,可能会导致另两种杂草的发生与蔓延。马齿苋尽管数量最多,但其在垂直方位的生态位宽度值最小,对玉米影响小,处于劣势。除草处理各经济性状指标值均较不除草对照高,其折合产量为10 111.11 kg/hm2,较不除草处理增产36.19%。     

Vehicle Monitoring Control and Scheduling Management System in a Scenic Area

In order to help tourists choose satisfying scenic spots, content hotels, convenient lines and convenient vehicles, vehicle monitoring control and scheduling management system in a scenic area is presented, which dispatches vehicle by appointments and the statistic of tourists at a sight spot and recommends the tour line by broadcasting in vehicle. Vehicle monitoring control and scheduling management system in a scenic area, a integrative vehicle management system, is an integration of GPS, GIS, trunked radio system, a management system of booking room and a tourist statistic system at a sight spot.     

Research on a Novel Inverter of VSC

In order to improve waveform performance of light HVDC system, a lot of switches which operate in high frequency are used, which causes large switching losses and results high cost. A new Buck type voltage source converter(BVSC) is proposed. The new BVSC comprises a dual-buck converter and a three-phase inverter operating in grid frequency. Only two high frequency switches are used, which results good output waveform performance. In order to reduce the high frequency switching losses, a novel improved double-frequency Buck type voltage source converter(DBVSC) is also proposed. An additional dual-Buck converter is added to the BVSC, the added dual-Buck converter deals with the fundamental power that operates in low frequency, and BVSC only deals with harmonic power that operates in high frequency. The output waveform is enhanced and the switching losses are reduced in DBVSC, and the power rating is also increased. The DBVSC is very suitable for large power rating application. The one cycle control theory is adopted to control the proposed DBVSC with analog circuit, which is very simple. The proposed DBVSC and control method are verified by simulation.     

Tentative Research About the Macroscopic Regulating to Blast

The paper analyzes the difference of the pathogenic varieties and the relationship of the variety composition,allording to the historic material to the varieties of the main hybid fece varieties in Southeast Sichuan during 1992 to 1998.It's the first time to advance to use the camparison on rate of pathogenic isolates between norm and refrieving to define the susceptible extent of the varity.It's an scientific base to adjust the structures of the vartieties,to reach the objective of the macroscopic regulating to rice blast.