Studies on Growth,Yield and Quality of Broccoli (Brassica Oleracea L.Var Italica Plenck) as Influenced by Boron and Molybdenum

Broccoli (Brassica oleracea L.var italica Plenck) is an important winter season exotic vegetable from the Brassicaceae family as well as heavy feeder of plant nutrients. Plant nutrition is one of the prime considerations for getting higher yield of any crop. Mineral fertilizer improves growth and yield of broccoli due to the role of nitrogen, phosphorus, and potassium on the meristematic activity. Boron and molybdenum are essential micronutrients required for normal plant growth and development. Plants differ widely in their requirements, but the ranges of deficiency and toxicity are narrow. Boron and molybdenum deficiencies are very common in Cole crops. Deficiency causes many anatomical, physiological, and biological changes. The deficiency of boron and molybdenum has threatened the ever increasing areas of broccoli. The effected heads become irregular in shape, smaller in size and bitter in taste which adversely affects the market demand of the crop. No systematic work so far has been done in production technology of sprouting broccoli in relation to response of nutrients in the gangetic plains of West Bengal. The present investigation was attempted to find out the growth, yield, and quality of broccoli on the aspects of minor mineral (boron and molybdenum) nutrition. The experiments were conducted at the Horticultural Research Station of Bidhan Chandra Krishi Viswavidyalaya, Mondouri, Nadia, West Bengal, during 2010–12 with four levels of boron (0, 15, 18, and 21 kg ha^?1) and molybdenum (0, 1.2, 1.5, and 1.8 kg ha^?1). Design of the experiment was a Factorial Randomized Block Design. Biometric observations were recorded from the selected plant from each plot and each replication of both the years. It was concluded from the results that under favorable agro-climatic conditions, the application of 18 kg borax and 1.8 kg ammonium molybdate ha^?1 was found beneficial for growth, yield, and quality of broccoli.     

Conservation and climate change: Assessing the vulnerability of snow leopard habitat to treeline shift in the Himalaya

Climate change is likely to affect the persistence of large, space-requiring species through habitat shifts, loss, and fragmentation. Anthropogenic land and resource use changes related to climate change can also impact the survival of wildlife. Thus, climate change has to be integrated into biodiversity conservation plans. We developed a hybrid approach to climate-adaptive conservation landscape planning for snow leopards in the Himalayan Mountains. We first mapped current snow leopard habitat using a mechanistic approach that incorporated field-based data, and then combined it with a climate impact model using a correlative approach. For the latter, we used statistical methods to test hypotheses about climatic drivers of treeline in the Himalaya and its potential response to climate change under three IPCC greenhouse gas emissions scenarios. We then assessed how change in treeline might affect the distribution of snow leopard habitat. Results indicate that about 30% of snow leopard habitat in the Himalaya may be lost due to a shifting treeline and consequent shrinking of the alpine zone, mostly along the southern edge of the range and in river valleys. But, a considerable amount of snow leopard habitat and linkages are likely to remain resilient to climate change, and these should be secured. This is because, as the area of snow leopard habitat fragments and shrinks, threats such as livestock grazing, retaliatory killing, and medicinal plant collection can intensify. We propose this approach for landscape conservation planning for other species with extensive spatial requirements that can also be umbrella species for overall biodiversity.     

Assessment of tillage erosion rates on steepland Oxisols in the humid tropics using granite rocks

Soil translocation by tillage may be an important factor in land degradation in the humid tropics. The objective of this study was to evaluate tillage-induced soil translocation on an Oxisol with 25% and 36% slopes in Claveria, Philippines for three tillage systems: contour moldboard plowing (CMP), moldboard plowing up and downslope (UMP), and contour ridge tillage (CRT). Small rocks 3–4 cm in “diameter” were used as soil movement detection units (SMDU). The SMDUs were placed at 10 cm intervals in a narrow 5-cm-deep trench near the upper boundary of each plot, the position of each rock recorded, and the trench backfilled. Five tillage operations used to produce one corn crop were performed during a one month period: two moldboard plowing operations for land preparation (except for CRT), one moldboard plowing for corn planting, and two inter-culture (inter-row cultivation) operations. After these operations, over 95% of the SMDU were recovered manually and their exact locations recorded. Mean annual soil flux for the 25% slope was 365 and 306 kg m⁻¹ y⁻¹ for UMP and CMP, respectively. For the 36% slope, comparable values were 481 and 478 kg m⁻¹ y⁻¹. Estimated tillage erosion rates for the 25% slope were 456 and 382 Mg ha⁻¹ y⁻¹ for UMP and CMP, respectively, and increased to 601 and 598 Mg ha⁻¹ y⁻¹, respectively, for the 36% slope. The mean displacement distance, mean annual soil flux, and mean annual tillage-induced soil loss for both slopes were reduced by approximately 70% using CRT compared to CMP and UMP.     

Transient co-expression with three <Emphasis Type="Italic">O</Emphasis>-glycosylation enzymes allows production of GalNAc-<Emphasis Type="Italic">O</Emphasis>-glycosylated Granulocyte-Colony Stimulating Factor in <Emphasis Type="Italic">N. benthamiana</Emphasis>

Background

Expression of economically relevant proteins in alternative expression platforms, especially plant expression platforms, has gained significant interest in recent years. A special interest in working with plants as bioreactors for the production of pharmaceutical proteins is related to low production costs, product safety and quality. Among the different properties that plants can also offer for the production of recombinant proteins, protein glycosylation is crucial since it may have an impact on pharmaceutical functionality and/or stability.

Results

The pharmaceutical glycoprotein human Granulocyte-Colony Stimulating Factor was transiently expressed in Nicotiana benthamiana plants and subjected to mammalian-specific mucin-type O-glycosylation by co-expressing the pharmaceutical protein together with the glycosylation machinery responsible for such post-translational modification.

Conclusions

The pharmaceutical glycoprotein human Granulocyte-Colony Stimulating Factor can be expressed in N. benthamiana plants via agroinfiltration with its native mammalian-specific mucin-type O-glycosylation.

     

Partnering with farmers to accelerate adoption of new technologies in South Asia to improve wheat productivity

There are many socioeconomic and technological constraints that affect the production of wheat and other staple cereals in South Asia. Wheat production is one of the economic mainstays in South Asia, but the yield gap between farmers’ fields and experimental yields is wide across the region. For the last 3 years, CIMMYT and the CAZS-NR have been collaborating with farmers, NARS, and other South Asian partners to promote improved wheat varieties and new resource conservation technologies (RCTs) in farmers’ fields. Participation fostered among farmers, scientists, extension specialists, NGOs and the private sector included variety selection (PVS), and evaluation of agronomic practices. Through PVS, several farmer-preferred technologies have been identified including wheat varieties for adverse conditions in eastern Uttar Pradesh (India) and for boron deficiency in parts of Nepal. There has been considerable improvement in the access of farmers to new varieties and technologies in the rural areas. Yield increases (15–70%) have been achieved by resource-poor farmers over the existing varieties through the adoption of new varieties and RCTs. The farmers have also made substantial cost savings and achieved higher yields through resource-conserving agronomic techniques such as zero till. Seed of the new farmer-selected cultivars has been multiplied by groups of collaborating farmers and widely distributed.     

EFFECT OF VENTILATION TECHNIQUE AND AIRWAY DIAMETER ON BRONCHIAL LUMEN TO PULMONARY ARTERY DIAMETER RATIOS IN CLINICALLY NORMAL BEAGLE DOGS

In dogs, a mean broncho‐arterial ratio of 1.45 ± 0.21 has been previously defined as normal. These values were obtained in dogs under general inhalational anesthesia using a single breath‐hold technique. The purpose of the study was to determine whether ventilation technique and bronchial diameter have an effect on broncho‐arterial ratios. Four healthy Beagle dogs were scanned twice, each time with positive‐pressure inspiration and end expiration. For each ventilation technique, broncho‐arterial ratios were grouped into those obtained from small or large bronchi using the median diameter of the bronchi as the cutoff value. Mean broncho‐arterial ratios obtained using positive‐pressure inspiration (1.24 ± 0.23) were statistically greater than those obtained at end expiration (1.11 ± 0.20) P = 0.005. There was a strong positive correlation between bronchial diameter and broncho‐arterial ratios for both ventilation techniques (positive‐pressure inspiration r_s = .786, P < 0.0005 and end expiration r_s = .709, P < 0.0005). Mean broncho‐arterial ratio for the large bronchi obtained applying positive‐pressure inspiration was 1.39 cm ± 0.20 and during end expiration was 1.22 cm ± 0.20. Mean broncho‐arterial ratio for the small bronchi obtained during positive‐pressure inspiration was 1.08 cm ± 0.13 and during end expiration was 1.01 cm ± 0.13. There was a statistically significant difference between these groups (F = 248.60, P = 0.005). Findings indicated that reference values obtained using positive‐pressure inspiration or from the larger bronchi may not be applicable to dogs scanned during end expiration or to the smaller bronchi.     

Phenotypic and genetic characterization of Erwinia rhapontici isolated from diseased Asian pear fruit trees

In 1995, a serious necrotic disease appeared in Asian pear trees in the orchards of Chuncheon, South Korea. Large numbers of bacterial samples were collected, and the causative microorganism was identified as a novel pathogen, Erwinia pyrifoliae. Among the samples, four bacterial isolates with atypical characteristics were further analyzed through biochemical tests and 16S rRNA gene sequence analysis. By phenotypic and genetic analysis these isolates were identified as E. rhapontici. Phylogenetic analysis using 16S rRNA sequence data revealed that E. rhapontici forms a discrete clade with high bootstrap value close to the E. amylovora group. Pathogenicity tests on leaves of tobacco plants (Nicotiana tabacum) elicited hypersensitivity responses, but artificial inoculations on immature fruits and shoots of Asian pear (Pyrus pyrifolia) did not show any necrotic symptoms. The developed primers showed no cross reactivity when tested against other phytopathogens and were able to detect E. rhapontici from mixed culture and in planta.     

Differences in the composition and diversity of bacterial communities from agricultural and forest soils

Differences in the bacterial communities of soils caused by disturbances and land management were identified in rRNA gene libraries prepared from conventional tilled (CT) and no tilled (NT) cropland, a successional forest after 30 y of regrowth (NF) and an old forest of >65 y (OF) at Horseshoe Bend, in the southern Piedmont of Georgia (USA). Libraries were also prepared from forests after 80 y of regrowth at the Coweeta Long Term Ecological Research site (CWT) in the Southern Appalachians of western North Carolina (USA). The composition of the bacterial communities in cropland soils differed from those of the Horseshoe Bend OF and CWT forest soils, and many of the most abundant OTUs were different. Likewise, the diversity of bacterial communities from forest was less than that from cropland. The lower diversity in forest soils was attributed to the presence of a few, very abundant taxa in forest soils that were of reduced abundance or absent in cropland soils. After 30 y of regrowth, the composition of the bacterial soil community of the NF was similar to that of the OF, but the diversity was greater. These results suggested that the bacterial community of soil changes slowly within the time scale of these studies. In contrast, the composition and diversity of the bacterial communities in the Horseshoe Bend OF and Coweeta soils were very similar. Thus, this forest soil bacterial community was widely distributed in spite of the differences in soil properties, vegetation, and climate as well as resilient to disturbances of the above ground vegetation.