Identification of non-alkaloid acetylcholinesterase inhibitors from Ferulago campestris (Besser) Grecescu (Apiaceae)

Inhibition of Acetylcholinesterase (AChE) is still considered as a strategy for the treatment of neurological disorders such as Alzheimer's disease. Many plant derived alkaloids (such as galantamine and rivastigmine) are known for their AChE inhibitory activity. Recently, other classes of natural compounds such as terpenoids, sesquiterpene glycosides and coumarins have been studied as new AChE inhibitors, with the aim to discover less toxic compounds compared to alkaloidal ones. The Ferulago campestris roots dichloromethane extract was used for a bioassay-guided fractionation for the search of AChE inhibitors. Three coumarin derivatives (umbelliprenin 1, coladonin 2 and coladin 3), three daucane ester derivatives (siol anisate 4, ferutinin 5 and 1-acetyl-5-angeloyl lapiferol 6), two phenol derivatives (2-epilaserine 7 and epielmanticine 8) and one polyacetylene (9-epoxyfalcarindiol 9) were isolated by the bioassay-guided approach. Their structures were characterized on the basis of spectral methods (1D and 2D NMR, and MS spectroscopy). All the isolated compounds were able to inhibit the AChE (IC₅₀ 1.2–0.1 mM) although at higher doses if compared to galantamine (6.7 μM) measured in the same conditions. The most active compounds were the daucane derivative siol anisate 4 and the epielmanticine 8, with IC₅₀ of 0.172 and 0.175 mM respectively.     

Characterization of Novel Selected Microalgae for Antioxidant Activity and Polyphenols,Amino Acids,and Carbohydrates

The biochemical composition of three novel selected microalgae strains (Chlorophyta) was evaluated to confirm their potential possibilities as new sustainably produced biomass with nutritional, functional, and/or biomedical properties. Extracts from cultured Pseudopediastrum boryanum, Chloromonas cf. reticulata, and Chloroidium saccharophilum exhibited higher radical scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazyl) when compared to butylated hydroxytoluene (BHT), but lower than butylated hydroxyanisole (BHA). Total phenolic compounds and amino acids were determined by newly developed RP-HPLC methods. Total phenolic contents, as µg g⁻¹ of dry biomass, reached 27.1 for C. cf. reticulata, 26.4 for P. boryanum, and 55.8 for C. saccharophilum. Percentages of total analysed amino acids were 24.3, 32.1, and 18.5% of dry biomass, respectively, presenting high values for essential amino acids reaching 54.1, 72.6, and 61.2%, respectively. Glutamic acid was the most abundant free amino acid in all microalgae samples, followed by proline and lysine in C. saccharophilum and P. boryanum, and methionine and lysine in C. reticulata. Soluble carbohydrates in aqueous extracts ranged from 39.6 for C. saccharophilum to 49.3% for C. reticulata, increasing values to 45.1 for C. saccharophilum and 52.7% for P. boryanum in acid hydrolysates of dried biomass. Results confirmed the potential possibilities of these microalgae strains.     

Pesticide residues used for pest control in honeybee colonies located in agroindustrial areas of Argentina

Abstract

With the aim of mitigating the negative impact of pests on agriculture, different agrochemicals are being used to maintain pest populations below an acceptable threshold of economic viability. The use of these chemical compounds, or even their misuse, can generate undesirable effects in the ecosystems and non-target organisms. World agricultural production depends on pollinators to generate 35% of food. Bees provide almost 75% of the pollination services worldwide. The objective of this study was to determine the agrochemical residues in honey obtained from Apis mellifera colonies located in agro-industrial regions of Argentina. Fifty honey samples form beehive pools of Santa Fe, Córdoba, La Rioja, Formosa, Entre Rios, Corrientes, Buenos Aires, Neuquén, and Río Negro provinces were analyzed. Out of all the samples analyzed, 88% had pesticide residues, being endosulfan sulfate the most widely chemical product reported. This information can be used to identify the provinces at higher risk for developing beekeeping activities, since the presence of these products in beehives can have a negative effect on this industry.     

Identification of Bartonella henselae in 2 cats with pyogranulomatous myocarditis and diaphragmatic myositis

Most cats infected with Bartonella henselae remain outwardly healthy carriers for years; however, self-limiting fever, transient anemia, neurologic dysfunction, lymphadenopathy, reproductive disorders, aortic valvular endocarditis, and neutrophilic myocarditis have been described in experimentally or naturally infected cats. Two cats in a North Carolina shelter died with pyogranulomatous myocarditis and diaphragmatic myositis. Bacteria were visualized in the lesions by Warthin-Starry silver impregnation and by B. henselae immunohistochemistry. B. henselae DNA was amplified and sequenced from the heart of 1 cat and from multiple tissue samples, including heart and diaphragm, from the second cat. This study supports a potential association between B. henselae and what has been historically described as "transmissible myocarditis and diaphragmitis" of undetermined cause in cats.     

Effects of serine palmitoyltransferase inhibition by myriocin in ad libitum-fed and nutrient-restricted ewes

The fungal isolate myriocin inhibits serine palmitoyltransferase and de novo ceramide synthesis in rodents; however, the effects of myriocin on ceramide concentrations and metabolism have not been previously investigated in ruminants. In our study, 12 non-lactating crossbred ewes received an intravenous bolus of myriocin (0, 0.1, 0.3, or 1.0 mg/kg/body weight [BW]; CON, LOW, MOD, or HIGH) every 48 h for 17 d. Ewes consumed a high-energy diet from day 1 to 14 and were nutrient-restricted (straw only) from day 15 to 17. Blood was collected preprandial and at 1, 6, and 12 h relative to bolus and nutrient restriction. Tissues were collected following euthanasia on day 17. Plasma was analyzed for free fatty acids (FFAs), glucose, and insulin. Plasma and tissue ceramides were quantified using mass spectrometry. HIGH selectively decreased metabolizable energy intake, BW, and plasma insulin, and increased plasma FFA (Dose, P < 0.05). Myriocin linearly decreased plasma very-long-chain (VLC) ceramide and dihydroceramide (DHCer) by day 13 (Linear, P < 0.05). During nutrient restriction, fold-change in FFA was lower with increasing dose (P < 0.05). Nutrient restriction increased plasma C16:0-Cer, an effect suppressed by MOD and HIGH (Dose × Time, P < 0.05). Myriocin linearly decreased most ceramide and DHCer species in the liver and omental and mesenteric adipose, VLC ceramide and DHCer in the pancreas, and C18:0-Cer in skeletal muscle and subcutaneous adipose tissue (Linear, P ≤ 0.05). We conclude that the intravenous delivery of 0.3 mg of myriocin/kg of BW/48 h decreases circulating and tissue ceramide without modifying energy intake in ruminants.     

Detection of Bartonella henselae in the blood of 2 adult horses

BACKGROUND: Bartonella spp. are emerging zoonotic agents that have been found in a wide variety of domestic animals and wildlife and cause a number of clinical syndromes. Bartonella sp. infection has been identified in a growing number of animal species, including cats, rodents, porpoises, and canids, but has not been reported in horses. OBJECTIVE: To document the presence of Bartonella sp. in the blood of horses. ANIMALS: One horse with chronic arthropathy and 1 horse with presumptive vasculitis. METHODS: Blood samples were tested for the presence of Bartonella sp. by a combination of multiplex real-time polymerase chain reaction and enrichment culture technique. RESULTS: Bartonella henselae was isolated or detected in the blood of both horses. CONCLUSION AND CLINICAL IMPORTANCE: Bartonella henselae infection should be investigated as the cause of disease in horses.     

Novel hemotropic Mycoplasma species in white-tailed deer (Odocoileus virginianus)

Globally, hemotropic Mycoplasma spp. are emerging or re-emerging zoonotic pathogens that affect livestock, wildlife, companion animals, and humans, potentially causing serious and economically important disease problems. Little is known about hemotropic Mycoplasma spp. prevalence, host-specificity, or route of transmission in most species, including wildlife. DNA amplification by PCR targeting the 16SrRNA and the RNaseP genes was used to establish the presence and prevalence of hemotropic Mycoplasma spp. in a white-tailed deer (O. virginianus) population in eastern North Carolina. Sixty-five deer (89%) tested positive for hemotropic Mycoplasma spp. where sequence analysis of the 16SsRNA and the RNaseP genes indicated the presence of at least three distinct species. This study represents the first detection of three distinct hemotropic Mycoplasma species in white-tailed deer and the first report of two novel hemotropic Mycoplasma species.     

Comparison of two models for estimation of variance components in a sample of Spanish Holstein Friesians

SUMMARY: Two models for estimating genetic parameters in animal populations were compared. The cheapest, in terms of computing costs, was based on a sire model and the most expensive on an animal model. In order to check the accuracy of both methods they were applied to the best available sample of Spanish Friesian cattle. Milk production of each cow, as well as fat contents were simulated using prior estimates of genetic additive, permanent environment and residual variances, keeping the actual mating scheme, so that the simulation mimicked the actual population structure. Results, under the above premises, show no advantages of the more costly procedure over the cheaper one, suggesting that a sire model based estimating procedure for genetic parameters may be preferred when a small number of individuals, little pedigree information and highly disequilibrated distribution of effects characterize the data. ZUSAMMENFASSUNG: Vergleich zweier Methoden der Varianzkomponentensch?tzung beim Spanischen Holstein Zwei Modelle zur Sch?tzung von genetischen Parametern wurden verglichen. Das im Hinblick auf die Rechenleistung günstigere Modell war ein Vatermodell, das aufwendigere Modell ein Tiermodell. Um die Genauigkeit der Modelle in der Praxis zu prüfen, wurden sie auf verfügbares Material der spanischen Holstein-Friesian Population angewendet. Sowohl die Milchleistung jeder Kuh als auch der Fettgehalt wurden simuliert mit Hilfe von Sch?t- zwerten der additiv-genetischen Varianz, der permanenten und der residuellen Umweltvarianz. Dabei wurde das tats?chliche Ungleichgewicht in der Population berücksichtigt. Unter den obengenannten Voraussetzungen zeigen die Ergebnisse keinen Vorteil der rechnerisch aufwendigeren Prozedur gegen?ber der einfacheren. Die Schlu?folgerung lautet, da? das Vatermodell unter den Bedingungen der kleinen Tierzahlen, wenig Pedigree-Informationen und einer stark unbalan- cierten Verteilung der Tiere auf die einzelnen Effektklassen zu bevorzugen ist.