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31.
Robert A. Mccleery 《Landscape Ecology》2009,24(4):483-493
Predator stimuli created by humans in the urban environment may alter animals’ anti-predator behaviors. I hypothesized that
habituation would cause anti-predator behaviors to decrease in urban settings in response to humans. Additionally, I hypothesized
that populations habituated to humans would show reduced responses to other predator stimuli. I observed three populations
of squirrels (urban, suburban and rural) responses to human approaches, red-tailed hawk vocalizations (Buteo jamaicensis) and coyote (Canis latrans) vocalizations. Mahalanobis distances of anti-predator behaviors in response to human approaches were consistent with the
urban–rural gradient. Flight initiation distances (X
2 = 26.33, df = 2, P < 0.001) and amount of time dedicated to anti-predator behavior (X
2 = 10.94, df = 2, P = 0.004) in response to human approaches were also consistent with the urban–rural gradient. Supporting the habituation hypothesis,
naive juvenile squirrels increased flight initiation distances (X
2 = 35.89, df = 1, P < 0.001) and time dedicated to anti-predator behaviors (X
2 = 9.46, df = 1, P = 0.002) relative to adult squirrels in the same urban environment. Time dedicated to anti-predator behaviors differed among
all three sites in response to both coyote (X
2 = 9.83, df = 2, P = 0.007) and hawk (X
2 = 6.50, df = 2, P = 0.035) vocalizations. Responses to both vocalizations on rural sites (coyote = 45%, hawk = 55%) greater than twice that
found on the urban sites (coyote = 11%, hawk = 20%). This is possibly the first case of a transfer of habituation demonstrated
under field conditions. 相似文献
32.
Sara Mello Pinho Giovanni Lemos de Mello Kevin M. Fitzsimmons Maurício Gustavo Coelho Emerenciano 《Aquaculture International》2018,26(1):99-112
Aquaponics is emerging as an alternative for high-health food production. Being able to identify the technical viability of non-conventional plants and fish species would help to increase the interest and possibilities in aquaponic systems. The goal of the present study was to evaluate the aquaponics production of two garnish species: scallion (S) and parsley (P), using effluents of pacu and red tilapia culture. Two aquaponics devices were used, differing according to the fish species, generating two different effluents. Thus, for plant performance, four treatments were evaluated in a factorial design (plant species and fish effluent as main factors), as followed: Pacu-S, Tilapia-S, Pacu-P, and Tilapia-P, with three replicates each, for 35 days. Fish performance was evaluated using Student’s t test. Each experimental device included a fish tank, filters, and six experimental units for the plants (floating rafts). Results indicated that feed conversion ratio (FCR) was higher in tilapia as compared to pacu (p < 0.05); however, fish productivity and survival were similar between species. Plant performance parameters were similar with no significant differences regardless of the fish effluent (p > 0.05), except for higher number of leaves per plant in scallion cultured using pacu effluent. Plant performance comparing both plant species indicated that scallion performed better as compared to parsley in all parameters. In addition, scallion also performed better related to the plant quality index. The results indicate that pacu presented a viable alternative for the aquaponics production, and regarding to the garnish, scallion performed better results as compared to parsley. 相似文献
33.
Within the framework of a larger project aimed to assess the potential of second generation hybrid striped bass for German
aquaculture the genotypic segregation of five microsatellite loci was analysed in two progeny lots (n = 74 and 76, respectively). There was no consistent correlation between microsatellite genotypes and phenotypic category
(white bass, hybrid, or striped bass). None of the individuals expressed neither only white bass nor only striped bass genotypes
at all five loci. On the other hand, only hybrid genotypes at all five loci were detected in three individuals of lot 1 and
four individuals of lot 2. Single loci tests for conformity of microsatellite genotypic segregation with Mendelian rules revealed
significant deviations (P < 0.05) in four cases for lot 1 and in three cases for lot 2. If pooled over all five loci, both lots displayed highly significant
deviations (P < 0.01) with an excess of hybrid genotypes and a deficiency of white bass genotypes. It is concluded that stabilizing selection
performed on hybrid genotypes might be a suitable approach for practical aquaculture in Europe if the goal is to become independent
of first generation hybrid fry supply and/or if establishing domesticated brood stocks of both parental species is impossible.
However, more detailed studies on the characteristics and performance of multiple hybrid generations are needed. 相似文献
34.
Kahori Arita Kiyoshi Isowa Takashi Ishikawa Hideo Aoki Hiromi Ohta 《Fisheries Science》2012,78(3):625-630
The post-thaw motility and fertility of Japanese pearl oyster sperm show large variances, even among sperm samples obtained
from the same individuals. This study aimed to clarify the factors that cause such differences. Spermatozoa were diluted 50
times with diluent comprising 10 % methanol, 18 % fetal bovine serum, and 72 % seawater, and dispensed into 0.25 ml straws.
A total of 59 straws were cooled, one by one, at 11 different heights from the surface of liquid nitrogen (LN) to −50 °C,
and then immediately immersed in LN. After thawing the straws, the relationships between the cooling rate and the post-thaw
motility and post-thaw fertility of the spermatozoa were examined. Both the post-thaw motility and the post-thaw fertility
showed a sharp peak when the straws were cooled at around −20 °C/min. There was a strong correlation between post-thaw motility
and fertility (P < 0.001). There was a large difference in the cooling rates and the post-thaw motilities and fertilities of the spermatozoa,
even between straws cooled at the same height. These results indicate that the optimum range for the cooling rate of oyster
spermatozoa is quite narrow, and the method of cooling straws at a fixed distance from the LN surface is unsuitable for the
cryopreservation of Japanese pearl oyster spermatozoa. 相似文献
35.
Nilnaj Chaitanawisuti Sirusa Kritsanapun Wannanee Santhaweesuk 《Aquaculture International》2011,19(5):865-872
A 120-day feeding trial was conducted to determine growth, feeding efficiency, and biochemical composition of juvenile spotted
babylon Babylonia areolata (initial mean weight of 1.48 ± 0.06 g) fed on conventional trash fish and a formulated moist diet in a flow-through grow-out
system. The snails readily accepted the formulated moist diet and showed good growth and survival during the trial. There
were no significant differences in specific growth rate, feeding efficiency, and survival between the snails fed on trash
fish or the formulated moist diet. At the end of the experiment, biochemical analysis of the snail flesh showed significant
differences in flesh quality, with the snails fed on trash fish having a significantly higher crude protein compared to those
fed on the formulated diet, but there was little difference in crude lipid content. Whole body tissue of snails fed on trash
fish had significantly higher total amino acid, essential amino acid, and non-essential amino acid levels compared to those
fed the formulated diet. This study showed that the formulated moist diet can be readily used for B. areolata growout for maximizing growth, survival, and feeding efficiency; however, further research is needed to improve the flesh
quality particularly amino acid and fatty acid levels before its suitability for B. areolata is assured. 相似文献
36.
Improved milk production through PG–PL system by provision of in‐house shelter management in lactating Murrah buffaloes during winter season 下载免费PDF全文
N. Haque M. Singh S. A. Hossain 《Journal of animal physiology and animal nutrition》2018,102(1):166-174
Comprehensive information on the role of β‐casein and plasminogen–plasmin (PG–PL) system in milk secretion of Murrah buffaloes during winter season is lacking, although effects of cold stress can be ameliorated to an extent by altering microclimate at farm level. Hence, this study was aimed to determine the changes in productivity along with PG–PL system of milk, plasma hormones and metabolites of buffaloes during winter (December–January) season under two different management systems. Average minimum temperature and wind chill index during this season were 7.02 and 12.74 °C respectively. Buffaloes were divided in two groups of six animals each: control and treatment, where treatment group animals were placed in‐house with floor bedding of paddy straw and the control group animals in loose housing system without straw bedding. Physiological responses were recorded, and milk and blood samples were collected at weekly intervals for six‐week experimental period. Under in‐house management system, buffaloes experienced better comfort by alleviating environmental stress as their physiological responses such as respiration rate and pulse rate were significantly reduced (p < 0.01) as compared to the control, which subsequently resulted higher milk yield by 9.92% (p < 0.05). Analysis of milk samples revealed higher concentration of plasminogen (10.6 vs. 8.05 μg/ml; p < 0.01) and β‐casein (p < 0.05), and lower plasmin level (0.299 vs. 0.321 μg/ml; p < 0.05) in buffaloes under treatment group. It was also found that plasma cortisol, glucose and non‐esterified fatty acids levels were higher (p < 0.01) in control group as compared to the treatment animals by 13.6%, 8.14% and 12.6% respectively. However, milk composition, growth hormone, epinephrine and norepinephrine level in plasma were similar in both the groups. Hence, it may be concluded that provision of in‐house shelter management with floor bedding of paddy straw during winter was effective to minimize environmental stress and improved milk production through manipulation of PG–PL system in buffaloes. 相似文献
37.
Concepta McManus Helder Louvandini Rosilene Gugel Luiz Cláudio Bastos Sasaki Eliandra Bianchini Francisco Ernesto Moreno Bernal Samuel Rezende Paiva Tiago Prado Paim 《Tropical animal health and production》2011,43(1):121-126
The objective of this study was to evaluate and compare physical parameters in groups of sheep with different phenotypic characteristics
in the Centre-west region of Brazil. Five groups of sheep, with nine animals per group, were selected, three groups of Santa
Inês animals with different coat colours (white, brown and black), one group with crossbred animals (Santa Inês × Bergamasca)
and one group with animals of the Bergamasca breed. The following traits were evaluated: coat thickness, number and length
of hair, pigmentation level in the coat and the skin as well as the percentage area of sweats glands in the skin tissue, carried
out by histological analysis. The number of hairs and the area of sweats glands were not significantly different between the
evaluated groups. The Bergamasca breed showed low pigmentation of the skin and long hairs. The levels of pigmentation of the
hair and of the skin were highly correlated. Between the Santa Inês groups, the group with white hair showed the better parameters
for heat adaptation, while the brown hair group showed the lower heat adaptation when compared with another hair breed groups. 相似文献
38.
Mi Jeong Park Jienny Lee Jeong Su Byeon Da-Un Jeong Na-Yeon Gu In-Soo Cho Sang-Ho Cha 《Veterinary research communications》2018,42(3):171-181
Mesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy fields. We optimize culture conditions of equine adipose tissue-derived MSCs (eAD-MSCs) for treatment of horse fractures. To investigate enhancing properties of three-dimensional (3D) culture system in eAD-MSCs, we performed various sized spheroid formation and determined changes in gene expression levels to obtain different sized spheroid for cell therapy. eAD-MSCs were successfully isolated from horse tailhead. Using hanging drop method, spheroid formation was generated for three days. Quantitative real-time PCR was performed to analyze gene expression. As results, expression levels of pluripotent markers were increased depending on spheroid size and the production of PGE2 was increased in spheroid formation compared to that in monolayer. Ki-67 showed a remarkable increase in the spheroid formed with 2.0?×?105 cells/drop as compared to that in the monolayer. Expression levels of angiogenesis-inducing factors such as VEGF, IL-6, IL-8, and IL-18 were significantly increased in spheroid formation compared to those in the monolayer. Expression levels of bone morphogenesis-inducing factors such as Cox-2 and TGF-β1 were also significantly increased in spheroid formation compared to those in the monolayer. Expression levels of osteocyte-specific markers such as RUNX2, osteocalcin, and differentiation potential were also significantly increased in spheroid formation compared to those in the monolayer. Therefore, spheroid formation of eAD-MSCs through the hanging drop method can increases the expression of angiogenesis-inducing and bone morphogenesis-inducing factors under optimal culture conditions. 相似文献
39.
Saxena MK Kumar AA Chaudhari P Shivachandra SB Singh VP Sharma B 《Veterinary research communications》2005,29(6):527-535
The applicability of ribotyping based on 16S and 23S rRNA was evaluated for molecular epidemiological studies. Forty-eight
isolates of Pasteurella multocida isolated from different hosts and geographical locations and one reference isolate were ribotyped. Only four ribotypes were
found. All the isolates including reference isolate from wild carnivores had the same ribotype, though they had different
serotypes. The isolate from a tiger had one band in addition to the bands present in the major ribotype. The isolates from
lions represented two ribotypes; of these ribotypes, one (r2) had an additional band of 3.6 kbp, which was absent in all other
ribotypes. The second ribotype (r4) from a lion had one band missing (6 kbp) that was present in the other ribotypes. These
isolates were further typed using ERIC-PCR and REP-PCR. With ERIC-PCR and REP-PCR, higher D values of 0.83 and 0.89 were obtained. The current study revealed that ribotyping is not a very efficient typing tool for
use in molecular epidemiology for differentiation of isolates. 相似文献
40.