Fruit Cracking in Mango (<Emphasis Type="Italic">Mangifera indica</Emphasis> L.) cv. ‘Dashehari’

The mango (Mangifera indica L.) is native to South and Southeast Asia, from where it has been distributed worldwide to become one of the most cultivated fruits in the tropics. It is the national fruit of India. In India, harvest and sale of mangoes take place during March-May and the fruits have high economic value in India. Studies were first time conducted on mango fruits to observe the severity of fruit cracking incidence. The stone was exposed in case of longitudinal type cracking and never exposed in star type cracking. The longitudinal cracking was 3.8, 3.4 and 1.7 % and star type cracking was 0.3, 1.8 and 0.02 % during 2009, 2010 and 2011, respectively. The lesser pulp thickness and higher stone weight in fruit cracked samples may have responsible for fruit cracking. There was a close relationship between fruit cracking and 3 months average weather conditions. The maximum temperature had positive relation with total fruit cracking.     

Identification of sugarcane grassy shoot-associated phytoplasma and one of its putative vectors in India

During a survey of sugarcane fields at the Sugarcane Research Institute, Shahjahanpur, Uttar Pradesh, India, in August–September 2012, 6% to 28% incidence of sugarcane grassy shoot disease was observed in different fields of sugarcane variety CoS 7250. The association of phytoplasma with symptomatic sugarcane was confirmed by direct and nested PCR amplification of phytoplasma ribosomal gene. Four different delphacid leafhopper species, viz. Cofana unimaculata Signoret, Exitianus indicus (Distant), Sogatella kolophon Kirkaldy and Hishimonus phycitis (Dist.) were the prevalent feeding species of the Auchenorrhyncha fauna in the symptomatic sugarcane fields. Out of these four leafhopper species, only E. indicus tested positive for phytoplasma presence. Phylogenetic analysis suggested that the phytoplasmas from sugarcane and E. indicus in the present study were members of 16Sr XI. The confirmation of association of sugarcane grassy shoot phytoplasma in E. indicus population is important to understand the secondary spread of this phytoplasma in sugarcane plants.     

Exploring Agrobiodiversity on Farm: A Case from Middle–Hills of Nepal

A survey was conducted to examine agrobiodiversity status of farms in the Pokhare Khola watershed of Dhading district in the Middle-Hills of Nepal. A total of 53 farmland tree and one crop species from 22 families were documented. The most commonly found species were fruit and fodder species (on over 60 % farms) including banana (Musa paradisiaca), khasrato (Ficus hispida) and khanayo (Ficus semicordata). Tree density was highest (226/ha) on marginal farms (farm area ≤0.25 ha) and lowest (165/ha) on small farms (farm area 0.26–0.5 ha). For the study area as a whole, the Shannon-Wiener species diversity index was 3.26 and the species evenness index 1.89. Large farms (farm area >1 ha) had the greatest tree species diversity (4.47 ± 0.52) and marginal farms the lowest (2.18 ± 0.37), indicating the positive relationship between farm size and species diversity. A total of six types of cereals and 18 types of vegetable crops were grown in the study area. The major livestock component of each household was chickens (average 8/household) and goats (6/household). The mean value of livestock in the large farm category was estimated as $2235, significantly higher than that of the other three categories. A significant relationship was found between agrobiodiversity and livelihoods, irrespective of annual production, and the critical role of the farmed landscape in agrobiodiversity conservation was apparent.     

Okra production with pine straw mulch

Conventional planted okra in Booneville, Arkansas and Lorman, Mississippi were mulched with loblolly pine straw (Pinus taeda L.) and longleaf pine straw (P. palustris Mill.), respectively, at a rate of 11 t/ha or left bare. At Booneville, plant stand, season yields (18.6 t/ha), pod weight (16.3 g), plant dry weight (2.3 kg), or stem diameter (3.5 cm) were not affected by the loblolly pine mulch. However, mulch application increased pod number (1.22 vs. 1.06×10⁶/ha) and plant height (1.5 vs. 1.6 m), while reducing weed competition (0.05 vs. 0.40 t/ha) and visible plant stress, during periods of soil moisture deficits. Soil temperatures at 5 and 15 cm depth were reduced by mulch until mid-August when plant canopies covered the rows. Seasonal moisture at 30 and 45 cm depths was similar between mulched and bare soils, based upon unreplicated neutron probe measurements. At Lorman, season okra yield (29.8 vs. 24.6 t/ha), number of pods per ha (1.24 vs. 1.07×10⁶) and weed competition were reduced and soil pH lowered 0.56 units by longleaf pine straw mulch. Mulch reduced early season yield at both locations.     

Haemorrhagic enteritis of turkeys – current knowledge

Haemorrhagic enteritis virus (HEV), an adenovirus associated with acute haemorrhagic gastro-intestinal disease of 6–11-week old turkeys predominantly hampers both humoral and cellular immunity. Affected birds are more prone to secondary complications (e.g. colibacillosis and clostridiosis) and failure to mount an effective vaccine-induced immune response. HEV belongs to the new genus Siadenovirus. Feco-oral transmission is the main route of entry of the virus and it mainly colonizes bursa, intestine and spleen. Both naturally occurring virulent and avirulent strains of HEVs are serologically indistinguishable. Recent findings revealed that ORF1, E3 and fib genes are the key factors affecting virulence. The adoption of suitable diagnostic tools, proper vaccination and biosecurity measures have restrained the occurrence of disease epidemics. For diagnostic purposes, the best source of HEV is either intestinal contents or samples from spleen. For rapid detection highly sensitive and specific tests such as quantitative real-time PCR based on Taq man probe has been designed. Avirulent strains of HEV or MSDV can be effectively used as live vaccines. Novel vaccines include recombinant hexon protein-based subunit vaccines or recombinant virus-vectored vaccines using fowl poxvirus (FPV) expressing the native hexon of HEV. Notably, subunit vaccines and recombinant virus vectored vaccines altogether offer high protection against challenge or field viruses. Herein, we converse a comprehensive analysis of the HEV genetics, disease pathobiology, advancements in diagnosis and vaccination along with appropriate prevention and control strategies.     

Duck virus enteritis (duck plague) – a comprehensive update

Duck virus enteritis (DVE), also called duck plague, is one of the major contagious and fatal diseases of ducks, geese and swan. It is caused by duck enteritis virus (DEV)/Anatid herpesvirus-1 of the genus Mardivirus, family Herpesviridae, and subfamily Alpha-herpesvirinae. Of note, DVE has worldwide distribution, wherein migratory waterfowl plays a crucial role in its transmission within and between continents. Furthermore, horizontal and/ or vertical transmission plays a significant role in disease spread through oral-fecal discharges. Either of sexes from varying age groups of ducks is vulnerable to DVE. The disease is characterized by sudden death, vascular damage and subsequent internal hemorrhage, lesions in lymphoid organs, digestive mucosal eruptions, severe diarrhea and degenerative lesions in parenchymatous organs. Huge economic losses are connected with acute nature of the disease, increased morbidity and mortality (5%–100%), condemnations of carcasses, decreased egg production and hatchability. Although clinical manifestations and histopathology can provide preliminary diagnosis, the confirmatory diagnosis involves virus isolation and detection using serological and molecular tests. For prophylaxis, both live-attenuated and killed vaccines are being used in broiler and breeder ducks above 2 weeks of age. Since DEV is capable of becoming latent as well as shed intermittently, recombinant subunit and DNA vaccines either alone or in combination (polyvalent) are being targeted for its benign prevention. This review describes DEV, epidemiology, transmission, the disease (DVE), pathogenesis, and advances in diagnosis, vaccination and antiviral agents/therapies along with appropriate prevention and control strategies.