乳酸菌对麻羽肉鸡生长和免疫机能的影响研究

选用1日龄温氏麻鸡母雏2400羽,随机分成3组,每组5个重复,每个重复160只鸡。试验Ⅰ组采用基础日粮+0.0125%黄霉素(4%);试验Ⅱ组采用基础日粮+1.5%乳酸菌制剂饮水;试验Ⅲ组采用基础日粮+1.5%乳酸菌制剂拌料后发酵6h。试验期72d,分为3阶段:前期(1～4周)、中期(5￣8周)、后期(9～11周)。结果表明:发酵方式添加乳酸菌组与抗生素组在生长性能和免疫机能方面无显著差异(P>0.05),但均比饮水组有所提高,发酵组可显著提高雏鸡盲肠内乳酸杆菌的数量,降低其内容物氨的浓度(P<0.05)。     

养殖池塘里套放小体积网箱养殖黄颡鱼试验

1试验材料和方法 1．1池塘条件 养殖池塘面积为8340m^2,长方形,条石池埂,东西走向,平均水深2．30m,水中泥沙含量较少,透明度35cm左右,周围没有工矿企业污染,溶氧充足,一般在5．5mg/l左右。4～10月份的水温在16～32℃之间,平均水温20℃。     

有机酸的抗菌效应及其机制的研究进展

有机酸添加到日粮中能够抑制胃肠道细菌对可利用养分的竞争作用和减少可能的细菌代谢产物的毒性作用,有机酸还能够抑制饲料和胃肠道中潜在病原菌(大肠杆菌和沙门氏菌)的定植和繁殖,从而有益于动物的健康。文中就有机酸的抗菌作用作一综述,并分析其作用机制和微生物对有机酸的耐受性。     

林业血防生态工程在血吸虫病防治中的作用及展望

1990年以来, 我国一些林业学者在总结长期生产和科研实践基础上提出了"以林为主, 抑螺防病"的林业血防生态工程科学设想, 即运用生态经济学原理, 在因地制宜的基础上, 采取以林为主, 生态学、环境学、农学、系统工程学以及流行病学等众多学科结合, 林、农、副、渔等多种经营, 创立融抑螺防病、经济、生态三效一体的人工林生态系统——抑螺防病林。通过多年的试验研究, 提出了多物种、多层次、多结构、多功能的低丘滩地的治理与开发模式以及不同类型的抑螺防病综合治理模式, 形成了以生物为主与工程措施相互结合的一整套较为系统的抑螺防病林体系。目前在长江流域湖区5省建立抑螺防病林试验点19个, 试验区面积6万多hm2, 抑螺防病效果十分明显。同时, 林业血防生态工程还兼具湿地保护、促进滩地综合治理与开发、有效降低滩地钉螺密度和促进林业产业发展等多重功能。     

Effect of L-lysine Added in Diluent on the Fresh Semen Quality of Dorper Sheep

In order to explore the effect of different L-lysine levels added in semen dilution on the fresh Dorper sheep quality stored at liquid state,3 health Dorper ram were used to collect semen which were equal-packaged and added to solution containing different levels (0,0.1,0.2,0.3,0.4 and 0.5 g) L-lysine in 120 mL diluent and at 0,6,24,30,48,54,72 and 78 h,the sperm motility,membrane integrity and acrosome integrity were detected.The results showed that the sperm motility in groups added 0,0.1 and 0.2 g L-lysine were higher than other groups,and that in 0.4 and 0.5 g L-lysine groups decreased faster than other groups;The sperm motility in 0.5 g L-lysine group reduced to 0 at 30 h,and it reduced to 0 at 48 h in the group added 0.4 g L-lysine;The effective survival time and survival index in the group added 0.1 g L-lysine was higher than other groups (P< 0.05);The membrane integrity of 0.1 g group was the highest,that had no significant difference with control group from 0 to 54 h (P >0.05),while after 72 h the two groups had significant differences (P< 0.05).The membrane integrity in 0.4 and 0.5 g groups were the worst,and that was significant different with other groups (P< 0.05);The acrosome integrity of 0.1 g group was the highest which had no significant difference with control group (P >0.05),0.4 and 0.5 g groups were the worst,the difference between them was not significant (P >0.05),while was extremely significant difference with other groups except for 0 and 6 h (P< 0.01).The results suggested that dilution added a high concentration of L-lysine could inhibit sperm quality,when the count of L-lysine was more than 0.2 g,sperm quality was significantly decreased,adding 0.1 g L-lysine could improve Dorper sheep fresh sperm quality stored at liquid state.     

动物园常态化保护教育项目的评估和分析——以“澳洲区幕后之旅”为例

保护教育作为现代动物园的主要职能之一,常态化的保护教育项目的开展正是动物园这一职能的重要体现.近两年南京市红山森林动物园开发了6个动物场馆幕后之旅的常态化教育项目,以澳洲区幕后之旅为例,通过对2014年4～7月的活动参与者进行问卷调查,对教育项目进行评估.结果显示此类教育项目切合当下亲子游的市场需求,对于影响常态化教育项目初期的正常开展的主要因素有市场推广、项目内容和师资力量.其中市场推广效果较好的是朋友推荐和网络宣传;项目内容中受欢迎程度较高的是亲近动物或动物相关内容;关于项目形式幼儿（4～6岁）偏好游戏互动环节而小学低年级（7～9岁）偏好动物故事环节.     

浅析河北省太行山区生态经济沟的可持续发展

河北省太行山区生态经济沟经过20多年的发展已取得了一定的成绩,在促进农业经济发展方面发挥了重要作用.面对新的形势,如何推进生态经济沟健康持续发展是一个重要课题.文章以河北省太行山区生态经济沟发展的现状为切入点,以生态经济相互协调为前提,以山区环境和资源、人口为依据,提出了建设农村高效农业,促进农村支柱产业发展等一系列建...     

FGF4在肺癌细胞体内和体外中表达差异的研究

FGF4已经被证明是癌基因,它涉及肿瘤的生长和转移,为了解FGF4的表达与肿瘤微环境的关系。我们利用FGF4抗体通过免疫组化对一名肺癌患者癌旁,癌组织,癌组织小鼠移植瘤,二次移植瘤以及原代培养的细胞爬片进行FGF4检测,探究其表达差异。通过对比癌组织与对照组（癌旁组织）,FGF4在癌巢中高表达;同样,移植瘤与二次移植瘤的癌巢中与癌旁组织比较,FGF4表达相对较高;但是将癌组织进行原代培养后,免疫组化检测细胞爬片FGF4,发现仅有5%±0.21%的肿瘤细胞表达FGF4,对照蛋白Cytokine作为肿瘤标记物,则在100%的肿瘤细胞中表达。研究提示免疫组化检测到FGF4在体内和体外表达不同,提示肿瘤细胞FGF4的表达与肿瘤微环境调控密切相关,肿瘤微环境对肿瘤细胞的FGF4的调控有着重要作用。     

Comparison of phenolic profiles and antioxidant activities in skins and pulps of eleven grape cultivars(Vitis vinifera L.)

Eleven grape cultivars were analysed to explore the variety differences of fresh grape phenolic profiles. The results showed that free phenolics were predominant in grape skins and pulps, and showed the higher antioxidant activities than bound. In 11 cultivars, Muscat Kyoho extracts had the highest total phenolic content in skins(10.525 mg GAE g~(–1) FW) and pulps(1.134 mg GAE g~(–1) FW), and exhibited the highest DPPH radical scavening capacity(EC_(50)=11.7 μg mL~(–1)) and oxygen radical absorbance capacity(ORAC) value(190.57 μmol TE g~(–1) FW) of free phenolic in skin. In addition, the most abundant phenolics in grape skins were found to be flavonoids such as kaempferol in Kyoho skin(541.2 μg g~(–1) FW), rutin, catechin and epicatechin in Muscat Kyoho skin(262.3, 86.3 and 70.0 μg g~(–1) FW, respectively). Furthermore, the principal component analysis showed a strong difference of phenolic profiles with the cultivars, existing forms and distributions. Pearson correlation coefficient analysis showed a significant linear correlation between total phenolic content and antioxidant activity(P0.05). Therefore, both skins and pulps were rich sources of bioactive phenolic compounds, and Muscat Kyoho was the ideal source among all samples.