基于MART模型的辽宁"数字林业"建设

电子政务是提高政府行政管理和公共服务能力的有效途径。“数字林业”是辽宁省林业现代化建设的重要内容，也是林业电子政务系统工程的重要组成部分。通过MART模型在辽宁林业体系中的研究应用，指导全省“数字林业”的开发与设计，实现有效整合，避免重复建设。     

Effect of lipoic acid on redox regulation,digestive and absorptive function in intestine of mice fed high fat diet

AIM:To elucidate the molecular mechanisms of lipoic acid (LA) on redox regulation and digestive function in intestine of C57BL/6 mice fed high fat diet (HFD).METHODS:C57BL/6 mice were randomly assigned to one of three groups (n=8).The control group consumed an ordinary diet.The other two experimental groups were fed with a high fat diet, high fat plus 0.1% LA.After 6 weeks, the activities of digestive enzymes were examined.In order to evaluate the antioxidant status of the mice, superoxide dismutase (SOD), malondialdehyde (MDA), total antioxidant capacity (TAC) and reactive oxygen species (ROS) in intestinal homogenate were measured.To investigate the molecular mechanisms underlying the effects of LA, the gene expression profiles in intestine were examined using the GeneChip microarray system.RESULTS:A depressed antioxidant defense system, accompanied by digestive and absorptive function impairment, was observed in HFD-fed mice.These changes were partially restored in the LA-treated group.DNA microarray analysis of intestine showed that LA ingestion up-regulated the expression of genes related to free-radical scavenger enzymes, digestive enzymes and transporters.CONCLUSION:Treatment with LA improves redox homeostasis and the function of intestine in mice fed HFD.The mechanism may involve preventing oxidative stress by scavenging ROS directly and increasing those of free-radical scavenger enzymes gene expression indirectly.     

水库网箱养殖锦鲤常见病害及防治

锦鲤是当今世界上最具有欣赏价值的观赏鱼类之一,具有“观赏鱼之王”的美称,是一种高贵的大型观赏鱼,它以其艳丽的体色、灿烂的斑纹、高雅优美的游姿、雄健英武的风度,博得了越来越多国内外广大养鱼爱好者的喜爱。笔者从2003年起与浙江省宁波市宁海欣观赏鱼养殖场合作,从香港引进锦鲤鱼苗进行水库网箱养殖。水库网箱养殖是近几年兴起的锦鲤养殖方式,由于养殖环境为开放式,受环境因子影响大,因此出现的病害也较多,现将笔者在养殖中常见的几种病害及防治方法介绍如下:     

盱眙铁山寺自然保护区植物区系及植物资源分析研究

在对盱眙铁山寺自然保护区进行植物调查和标本采集的基础上,分析了该地区植物区系基本组成成分及其植物区系特点。并对植物资源进行了分析,按其经济用途,分为纤维植物、淀粉和糖类植物、油脂和芳香油植物、药用植物、野菜植物和观赏绿化植物6大类。最后提出了植物资源开发利用和保护的建议。     

Ni—Si—B系非晶合金的结晶度与退火时间的关系

对Ｎｉ６４Ｓｉ１５Ｂ２１非晶合金条带在２５０℃不同退火时间下的结晶度进行了测定。得到了结晶与度火保温时间呈抛物线关系的实验规律。     

一个对播种期反应敏感的不育大豆特征分析

Brim和Young最早发现并报道了大豆雄性不育、雌性可育突变体,即msl系统(Brim and Young 1971)。其特点是雌性大部分可育,雄性几乎完全不育;产生大量双胚苗(Kenworthy等,1973);由减数分裂的异常引起(Albertsen,1976)。此外,在大豆中还发现了其它完全或部分雄性不育突变。这包括Arkansas部分雄性不育(Cavines等,1970;Cavines and Fagala,1973)、ms2突变休(Bernard and Cremeens,1975)和Wabash雄性不育(Chaudhari and Davis,1977)。Palmer证实,在msl位点的4个独立突变均引致雄性不育(Reid G. Palmer等,1978)。作者曾对一个核质互作雄性不育大豆的发现、特征及表现进行了描述,并进行初步遗传分析(Yuhua Peng el al.,1993)。本文对一新的不育材料进行了详细的描述和分析。     

含钛高炉渣水解制取钛白的动力学研究

在实验基础上确立了含钛高炉渣水解制取钛白过程中钛液ＴｉＯＳＯ４浓度随时间的变化关系，经数学分析建立了动力学方程并讨论了方程的适应性。为信水解过程听进一步研究和生产实践提供了数据。     

Mass mortalities associated with viral nervous necrosis (VNN) disease in two species of hatchery-reared grouper, Epinephelus fuscogutatus and Epinephelus akaara (Temminck & Schlegel)

Mass mortalities of hatchery-reared juvenile groupers have occurred in southern Taiwan. The diseased fish swam in a darting, corkscrew fashion. Light microscopy revealed vacuolation in the brain tissue. Electron microscopy showed numerous non-enveloped, cytoplasmic viral particles (20–25 nm in diameter) in the brain cells, and many virions were enclosed in the membrane-bound organelles of the cells. Two structural proteins of the purified grouper virus, with molecular weights of 44 and 43 kDa, were revealed by SDS-PAGE. Moreover, the results of RT-PCR and nested PCR diagnosis using primers specific to the T2 and T4 target segments of striped jack nervous necrosis virus (SJNNV) RNA2 genes suggest that this virus is a fish nodavirus, and is designated as GNNV 9410 strain (grouper nervous necrosis virus strain 9410). This is the first case report of viral nervous necrosis among marine fish in Taiwan.     

中国南方荷斯坦奶牛κ-CN基因第四、第五外显子的多态性研究

本文利用PCR-RFLP技术，检测了中国南方荷斯坦牛κ-酪蛋白（κ-casein，κ-CN）基因第4、第5外显子的遗传多态性。结果表明：κ-CN基因第4、第5外显子均存在遗传多态性，第4外显子上存在突变位点g．10943A〉C，第5外显子上存在突变位点g．15153T〉C。第4外显子有两种基因型（AA，AB），等位基因频率分别为0．9379和0．0621，第5外显子有三种基因型（AA，BB，AB），等位基因频率分别为0．6512和0．3488。κ-CN两个外显子PCR-RFLP位点的基因型分布符合Hardy-Weinberg平衡定律（p〉0．05）。κ-CN基因第4、第5外显子座位的多态信息含量分别为0．1097和0．3511，第4外显子呈现低度多态，第5外显子呈现中度多态；其杂合度、有效等位基因数分别为0．1165、1．1318和0．4543、1．8324。