纽荷尔脐橙缺硼表现与其硼、糖含量年变化的关系

 研究了赣南‘纽荷尔’和‘朋娜’脐橙果实及叶片的硼和可溶性糖含量的年变化。结果表明: 幼果期两品种果皮硼含量均较高, 之后果皮与果肉硼含量均趋下降, 但在果实膨大中后期均出现显著上升。纽荷尔越冬老叶硼含量趋明显下降并居较低水平, 而朋娜老叶硼含量变幅小且相对较高。果实膨大中后期两品种果皮和果肉的可溶性糖含量均与果实硼含量出现同步积累, 此时二者老叶和朋娜春梢叶的糖含量均出现低谷, 而对应纽荷尔春梢叶糖含量并无明显下降。     

香蕉茎尖的玻璃化法超低温保存及其植株再生

 以香蕉(Musa spp. ) 为试材, 对其离体培养茎尖玻璃化法超低温保存影响因素进行研究。结果表明, 不定芽在MS + 3.0～5.0 mg/L 6-BA + 0.1 mg/L NAA的培养基上分化较好。香蕉茎尖超低温保存较佳体系是: 2.0～3.0 cm的茎尖在含0.4 mol/L蔗糖培养基上预培养2 d, 剥取带1～2个叶原基的茎尖(长1.0～1.5 mm) , 室温(25℃) 下装载液(MS + 2 mol/L甘油+ 0.4 mol/L蔗糖) 装载20～30 min, 然后用玻璃化溶液( PVS2 ) 于0℃下处理40 min, 换1次PVS2后迅速投入液氮。保存至少1 h后, 在40℃水浴中化冻90 s, 用1.2 mol/L蔗糖培养液洗涤2次, 每次10 min, 然后转入含0.3 mol /L蔗糖的MS培养基上,暗培养10～15 h后转移到含0.5 mg/L 62BA的MS培养基中, 暗培养1周后转移到正常光下, 3个香蕉品种(巴西蕉、广东香蕉2 号、广东粉蕉1 号) 的成活率分别为75.9%、40.0%和69.6% , 再生率分别为63.4%、35.0%和63.4%。再生植株生长和分化正常, 生根后可移栽成活。     

酚类物质与蝴蝶兰褐变关系初探

 利用高效液相色谱法对3个褐化程度不同的蝴蝶兰品种A1 (大白花红心) 、B3 (迷你型白花黄心) 和R4 (深红花红心) 进行了9种酚酸定性定量分析, 并研究了以叶片为外植体的初代培养过程中总酚含量的动态变化。结果表明: 绿原酸、邻苯二酚、儿茶酚、咖啡酸及没食子酸、对羟基苯甲酸、香豆酸可能与蝴蝶兰褐变相关, 苯甲酸对蝴蝶兰褐变影响很小; 在褐变过程中, 总酚含量越高, 褐变程度越严重。     

魔芋葡甘聚糖在环保、医药及食品中的应用

对魔芋葡甘聚糖的特性及在环保、医药、食品及其他领域中的广阔应用进行了综述,讨论了魔芋葡甘聚糖的环保性能、药理作用、食品加工等方面的应用前景.     

节瓜新品种夏冠1号的选育

夏冠1号是以自交系7213作母本,以自交系1632作父本配制而成的节瓜一代杂种。植株生长势较强,分枝性强,主侧蔓结瓜。早熟,春栽从播种至始收65~80d(天),夏秋栽从播种至始收45~55d(天)。瓜圆筒形,皮青绿色,有光泽,被茸毛,无棱沟,瓜长16~17cm,横径5.5cm,单瓜质量450~500g。肉质嫩滑,微甜。耐热、耐寒能力较强,高抗枯萎病,对疫病的抗性强于对照江心4号。每667m2产量3000~4000kg,适宜华南地区春、夏、秋季种植。     

Effect of TLR4 activation on endothelium adhesion function and atorvastatin intervention

AIM：To investigate the modulation of LOX-1 and monocyte-endothelium adhesion by TLR4 activation in human umbilical vein endothelial cells (HUVECs),and explore LOX-1’s role in mediating monocyte-endothelium adhesion,and the effect of atorvastatin.METHODS：TLR4 and LOX-1 mRNA were measured by RT-PCR.The expression percentage of TLR4 and LOX-1 positive cells were detected by flow cytometry.The adhesive percentage between monocytes and HUVECs were determined by counting.RESULTS：Incubation by LPS (1 mg/L) for 24 hours upregulated TLR4,LOX-1 mRNA and protein expression in HUVECs，and increased the percentage of monocyte adhesion to endothelium.Pretreatment of cell with anti-LOX-1 partly abolished the increase of monocyte adhesion to endothelium.Atorvastatin (10 μmol/L) inhibited LPS-mediated effects above.CONCLUSION：TLR4 activation upregulates LOX-1 expression and increases monocyte-endothelium adhesion.LOX-1 partly involves in LPS-induced monocyte-endothelium adhesion,atorvastatin may have protective effects on endothelium by inhibiting TLR4 and TLR4-induced LOX-1 expression.     

Mechanism of therapeutic effect of QingKaiLing-ingredient combination on focal cerebral ischemia-reperfusion injury

AIM:To elucidate the therapeutic effect and mechanism of QingKaiLing (QKL)-ingredient combination on cerebral ischemia. METHODS:Rat focal brain ischemia reperfusion injury was produced by occlusion in the middle cerebral artery. MRI and histological analysis were used to evaluate the brain injury induced by ischemia and the attenuations by the drugs including QKL-ingredient combination and the positive control nimodipine. In situ hybridization was used to measure the expression of BDNF mRNA in the brain. RESULTS:A significant neuroprotective effect was observed in the QKL-ingredient combination treatment group, which was superior to that in the nimodipine treatment group. The expression of BDNF mRNA in the brain was also significantly up-regulated by the QKL-ingredient combination treatment in a rat model of focal cerebral ischemia-reperfusion injury. CONCLUSION:The QKL ingredients show a neuro-protective effect, the mechanism may be related to the up-regulation of BDNF in the brain.     

Effect of endogenous H2S on pulmonary hypertension during acute lung injury induced by LPS and its interaction with NO

AIM: To investigate the effect of H2S on pulmonary artery hypertension during acute lung injury induced by LPS and the interaction between the systems of hydrogen sulfide (H2S)/cystathionine-β-lyase (CSE) and nitric oxide (NO)/nitric oxide synthase (NOS) in this process. METHODS: Seventy-two adult male rats were randomly divided into four groups: control group, LPS group, LPS+L-NAME group and LPS+propargylglycine (PPG) group. Mean pulmonary artery pressure (mPAP) of each rat was examined at 2 h, 4 h, 6 h and 8 h after treatment. H2S and NO contents in plasma, NO content, iNOS, cNOS and CSE activity in lung were measured at 4 h or 8 h after treatment, respectively. Expression of iNOS in lung tissue was also detected by immunohistochemistry technique, and the injury of lung was evaluated with morphological changes under microscope. RESULTS: LPS could induce severe lung injury, and mPAP, NO content, iNOS activity and its protein expression in LPS group significantly increased, but cNOS activity, H2S content and CSE activity decreased compared with those of control group. Administration of L-NAME before LPS could attenuate the changes induced by LPS. Pre-administration of PPG, a CSE inhibitor, exacerbated the injury by LPS, but there was no prominent variation in cNOS activity. CONCLUSION: Reduced endogenous H2S could increase pulmonary artery hypertension during acute lung injury induced by LPS. There is a negative effect between H2S/CSE system and NO/NOS system in this process.     

转基因植物对蜂类昆虫安全性

随着转基因植物的大规模商业化种植,其对蜂类等有益昆虫的影响成为研究转基因生物风险评估的主要内容。一方面蜜蜂等蜂类取食植物花粉,有可能直接接触到杀虫蛋白,产生毒性效应;另一方面转基因植物的性状、营养成分等可能发生变化,对蜜蜂的行为可能产生潜在影响。本文综述了目前转基因植物对蜂类昆虫安全性研究进展,并提出克服转基因植物对非靶标生物可能的负面效应的治理对策,以期对转基因植物生物安全性的研究提供科学依据。