Isolation and Identification of BPIV3 Genotype C

To identify the infection agents from Ningxia Hui Autonomous region, where feedlot cattle indicated bovine respiratory disease complex (BRDC), the M gene of the bovine parainfluenza virus type 3 was amplified by RT-PCR.The PCR product was ligated to pMD18-T vector and cloned to E.coli DH5α.The positive clones were sequenced and compared with the reference strains in GenBank by the molecular biology software.Sequence alignment results showed that a BPIV3 strain was isolated from the samples and named NX49, the M gene of NX49 included 1 056 nucleotides.Evolutionary analysis showed that the NX49 belonged to BPIV3 C genotype and shared 99.4% nucleotide identity with that of the SD0835 isolated in Shandong province.The characterization of the NX49 demonstrated that it was sensitive to temperature, acid and organic matter.The presence of Mg²⁺ showed no protection against the treatment at high temperature.The HA test suggested that the NX49 enables to agglutinate the guinea pig RBC at 4 ℃ and the titer was 1∶4.The study isolated a BPIV3 genotype C strain successfully, which facilitate the study of molecular evolution and epidemiology of BPIV3 in China.     

Analysis on Polymorphisms of Three CNV Regions in Five Pig Breeds

The paper was aimed to investigate the polymorphism of copy number variation (CNV) in different pig breeds.Three CNV regions of CNVR91,CNVR92 and CNVR143 were chosen from the porcine SNP60 chip genotyping results.The polymorphisms of three CNVs were determined by Real-time quantitative PCR method,taking five pig breeds as samples,including Yorkshire pig,Xiang pig,Kele pig,Nuogu pig and Rongchang pig breeds.The results showed that the dominant status of CNVR91 was loss in Xiang pig,while it was normal in other four pig breeds.The major type of CNVR92 was deletion in Xiang pig,Yorkshire pig,Kele pig and Rongchang pig breeds,with a high normal percent in Nuogu pig.For CNVR143,the dominant event was gain in Xiang pig and Nuogu pig breeds,but it was not diverse in other three pig breeds.These results indicated that three CNV regions emerged with polymorphism in five pig breeds,which might have effects on gene expression in CNV regions and physiological function by dosage effect especially in Xiang pig,Nuogu pig and Kele pig breeds.     

管理措施对三江源区"黑土滩"土壤肥力及土壤酶活性的影响

对三江源区典型退化草地“黑土滩”采取施肥及种植垂穗披碱草的恢复措施,结果表明,土壤中的全氮与有机质的相关性显著,全磷与有机质的相关性较明显,变化趋势相一致。短期的恢复措施造成了恢复样地20~30 cm土壤层中有机质、全氮和全磷含量的下降,长期的恢复措施使恢复样地中的这3种营养物质的含量得到了上升;人工种植措施使土壤中的全钾含量显著下降(P<0.05)。此外,速效养分的变化不一致,其中速效氮的含量表现为先降低后升高,速效磷和速效钾的含量变化表现出升高趋势。植被恢复对土壤脲酶活性有促进作用,但蔗糖酶活性没表现出规律性变化;这2种酶的活性与速效养分的含量表现出较强的正相关,而与有机质和全价养分的含量相关性不显著。     

Reuolution and Research of Green Automobile

The green is as a symbol of no pollution,no public harm and environmental protection. The green automobile is the inevitable results that the automobile technique continuously developes. A new style vehicle--the green automobile is the urgent solicit of environmental protection is developed. The automobile industry can develop continuously. This article mainly discusses the importance of the green auto_mobile's research and development,the development status in quo and prospect of the green auto_mobile technique in the world,and states the relationship between the green automobile technique and environmental protection from green technical system theoretically clarify the study of green automobile,including its design and the system,the manufacture and its technology,the energy,the rebuild engineering and a series of subjects. How to evaluate the cycle and the evaluable mark of the green automobile,the prospect of green automobile are pointed out.     

An Estimate on the Casual Relation between Financial Development and Economic Growth

This paper firstly discusses the relationship among money,real interest rate and economic growth of China. Then estimates the causality between financial development and economic growth by Bivariate Granger Casual Relation Test. The conclusion suggests that economic growth stimulate financial development more in China.     

不同颜色粘虫板对葡萄园昆虫的诱集效应

为筛选出对葡萄害虫诱集效果好的粘虫板,选用黄色、蓝色、白色、紫色、绿色、红色、黑色和荧光色8种颜色的粘虫板对葡萄园内昆虫进行诱集试验。结果表明,共诱到昆虫7目18科22种,主要害虫有蓟马、蚜虫和叶蝉,其中蓟马数量占到昆虫总量的88.96%,天敌昆虫主要有寄生蜂、捕食性瓢虫、食蚜蝇和东亚小花蝽。紫板、蓝板和白板对蓟马的诱集效果强,黄板、荧光板和绿板次之,红板和黑板效果差;黄板对蚜虫诱集效果强,绿板和荧光板次之,黄板与其他色板之间差异显著;黄板、荧光板和绿板对叶蝉的诱集效果好,3种色板与蓝板之间差异不显著、与其他色板差异显著。白板、蓝板和黄板诱集到的天敌数量最多,占所有颜色粘虫板诱集天敌总量的69%左右。试验期间诱集到的昆虫益害比为0.036,各色板的益害比依次为黑色>荧光色>绿色>红色>黄色>白色>蓝色>紫色,其中紫板的益害比最低,仅为0.011。结合各色板的诱虫效果和益害比进行综合分析,建议选用紫板对蓟马进行诱杀,适时适量选择性使用黄板对蚜虫、叶蝉等进行诱杀。     

小型密集式烤房与普通气流下降式烤房的对比试验

以原烤房为基础改成增大容量的密集烤房,采用烤烟品种云烟87为材料,对气流下降式与小型密集烤房的烘烤成本和经济效益进行了对比分析。结果表明:每1 kg干烟叶小型密集烤房比普通气流下降式节约0．59元;每烘烤1炕烟叶比气流下降式烤房节约成本148．68元;667m2产值多137元。小型密集烤房是解决种烟大户和专业户烤烟烘烤的理想烤房。     

联苯肼酯与2种杀螨剂复配对柑橘全爪螨的联合毒力

柑橘全爪螨是一种世界性害螨, 目前对许多药剂已经产生抗性。药剂复配是治理抗性问题, 延长药剂使用寿命的重要手段。为筛选出增效明显的复配新产品, 本文选取联苯肼酯作为标准药剂, 与阿维菌素和氟啶胺分别进行复配, 并采用叶碟喷雾法测定其联合毒力及增效作用。结果表明, 联苯肼酯、阿维菌素和氟啶胺的单剂LC50分别为16.43、0.29 mg/L和12.63 mg/L。联苯肼酯与阿维菌素复配比例为19∶1和15∶5的共毒系数分别为135.02和158.40, 具有增效作用。联苯肼酯与氟啶胺在6∶4、7∶3、5∶5、9∶1、8∶2复配比例的共毒系数分别为466.28、410.61、370.22、311.15和275.85, 增效作用显著。     

北京鹫峰地区松栎混交群落结构与物种多样性

利用在北京鹫峰地区设置的8个样方的调查资料,对该地区松栎混交群落结构及物种多样性进行了分析研究。结果表明:群落土壤呈微酸性,自然肥力较低;调查共计维管束植物58种,隶属于36科56属,其中乔木树种占主导地位,植株的最大密度为1 975株/hm2;生活型谱以高位芽植物最多,1年生草本植物比例居中,反映出群落水分条件一般;群落结构分明,乔木层、灌木层和草本层层次清晰;乔木以栓皮栎占绝对优势,其重要值最高,优势度明显;乔木层物种多样性指数不高,群落物种丰富度和多样性指数主要受灌木层和草本层影响,群落处于恢复前期;群落的物种丰富度、物种多样性指数和均匀度指数反映出基本一致的趋势,不同层次的Shannon-Wiener多样性指数、Simpson优秀度指数、种间相遇机率和Pielou均匀度指数的趋势基本上是草本层>灌木层>乔木层。     

德国可再生能源发展的借鉴与启示

近年来,可再生能源逐渐成为世界各国关注的热点,并成为各国应对气候变化以及生态环境保护的共同选择,其在经济发展以及维护生态环境中开始被重视起来.德国能源转型的成功已经成为欧美国家中的典范,为此农业农村部科技教育司组团赴德国参加"农业废弃物资源化利用和农村清洁能源综合利用技术"培训项目,笔者结合培训内容和实地考察形成该篇文...