影响锤片式粉碎机工作性能的主要因素

<正>粉碎工段是饲料厂生产的关键工段之一,是饲料厂的耗能大户,该工段工作的好坏直接关系到饲料厂的质量、产量、成本。随着饲料工业的高度发展,大量的锤片式粉碎机被饲料厂选用,但在使用中出现许多问题,现在我就把影响锤片式粉碎机工作性能的主要因素简单地分析一下,以供大家参考。     

安徽省一起非洲猪瘟疫情的暴发调查

2018年9月,安徽省铜陵市某猪场暴发非洲猪瘟疫情。为查明疫情可能的来源、波及范围,继而提出有针对性的预防控制建议,对该猪场的饲养管理、猪群发病现状以及相关流行病学关联场点进行了现场调查。调查发现,此次暴发可能由饲喂泔水引起。由此建议:暂时关闭辖区内生猪屠宰场,并进行彻底消毒;停止疫情相关区域生猪及其产品调运;监测受威胁区猪场,做好生物安全防护;停止饲喂泔水,并做好餐厨剩余物的无害化处理工作。     

家蚕丝质性状选择效果研究

本文采用系统选择、杂交改良等方法。对解舒、净度、强伸力、茧丝长等家蚕主要丝质性状进行了研究。结果表明,解舒、净度、茧丝长系统选择与杂交改良是有效的;对解舒率和茧丝长进行严格综合系统选择,在一定程度上能克服解舒率与茧丝长的负相关关系;系统选择法所建立的解舒好系与解舒差系能稳定遗传;解舒率与强力、伸长度成正相关,但相关程度很低,一代杂种的强力高于原种。     

Expression and functional analysis of the Follistatin-like 3 (FSTL3) gene in the sheep ovary during the oestrous cycle

Follistatin-like 3 (FSTL3) is a regulator of cellular apoptosis and was previously identified via RNA-Seq to be associated with follicular development in mammalian ovaries. However, the mechanism underlying the FSTL3 regulation of oestrus in sheep remained poorly understood. In this study, the oestrogen (E2) and progesterone (P4) concentrations in blood were detected, and the expression level and functional analysis of FSTL3 in the ovary were studied during the different reproductive stage in Aohan fine wool sheep (seasonal breeding breed in China). The concentrations of E2 and P4 at the anestrus were significantly lower compared to dioestrus, proestrus and oestrus stages. Higher expression levels of FSTL3 were observed in the sheep ovary, hypothalamus, and thyroid. During different reproductive stages, higher expression levels were found during the stages of dioestrus and proestrus, while lower levels were found during the oestrus and anestrus stages. Functional analysis of FSTL3 was performed in primary granulosa cells (GCs) of sheep. The concentration of E2 increased significantly after RNAi interference of FSTL3, while the P4 level decreased. FSTL3 can decrease P4 levels, which might be involved in mediating oestrous cycle in sheep.     

猪流行性腹泻病毒实时荧光定量RT-PCR检测方法的建立

根据Gen Bank报道的N基因高度保守核苷酸序列,设计并合成一对引物。上下游引物与Gen Bank中登录的153株猪流行性腹泻病毒(PEDV)N基因全长序列匹配度分别是100%和97%。以本实验室分离流行毒株为模板,利用SYBR Green I荧光染料法进行RT-PCR扩增,获得扩增产物构建重组质粒作为阳性对照,建立检测猪流行性腹泻病毒核酸的方法。同一样品进行3次重复试验,变异系数0.9%。通过对临床样品进行检测和测序验证,核酸检测结果中的阳性样品准确率为100%。本研究所建立的荧光定量PCR检测方法具有快速、灵敏、准确等优点,可用于临床PEDV的检测及分子流行病学调查。     

Pharmacokinetics and bioavailability of valnemulin in broiler chickens

Wang, R., Yuan, L.G., He, L.M., Zhu, L.X., Luo, X.Y., Zhang, C.Y., Yu, J.J., Fang, B.H., Liu, Y.H. Pharmacokinetics and bioavailability of valnemulin in broiler chickens. J. vet. Pharmacol. Therap. 34 , 247–251. The objective of this study was to investigate the pharmacokinetics and bioavailability of valnemulin in broiler chickens after intravenous (i.v.), intramuscular (i.m.) and oral administrations of 10 mg/kg body weight (bw). Plasma samples were analyzed by high‐performance liquid chromatography–tandem mass spectrometry (HPLC‐MS/MS). Pharmacokinetic characterization was performed by non‐compartmental analysis using WinNonlin program. After intravenous administration, distribution was wide with the volume of distribution based on terminal phase(V_z) of 4.27 ± 0.99 L /kg. Mean valnemulin t_1/2β(h), Cl_β(L /h /kg), V_ss (L /kg) and AUC_(0–∞)(μg·h /mL) values were 2.85, 0.99, 2.72 and 10.34, respectively. After intramuscular administration, valnemulin was rapidly absorbed with a C_max of 2.2 μg/mL achieved at 0.43 h (t_max), and the absolute bioavailability (F) was 88.81%; and for the oral route the same parameters were 0.66 ± 0.15 μg/mL, 1.54 ± 0.27 h and 74.42%. A multiple‐peak phenomenon was present after oral administration. The plasma profile of valnemulin exhibited a secondary peak during 2–6 h and a tertiary peak at 32 h. The favorable PK behavior, such as the wide distribution, slow elimination and acceptable bioavailability indicated that it is likely to be effective in chickens.     

我国茸鹿育种研究进展及现代茸鹿育种探讨

综述了我国茸鹿育种自 1 958年始至今历经 42年 3个阶段 ,成绩显著 ,取得了令世界瞩目的成就     

益生菌对鲤鱼免疫功能影响的研究

本研究采用有益芽孢杆菌制成微生物添加剂 ,对鲤鱼的生长和免疫功能进行试验。试验鲤鱼苗分两组 ,试验组添加1 %生态制剂 ,经100d饲喂试验 ,结果 :①益生菌生态制剂对鲤鱼有促生长作用 ,试验组较对照组提高增重11.8 % ,饵料系数下降0.24 ;②试验组免疫器官胸腺、脾脏生长发育较对照组迅速、成熟快 ,电镜观察免疫器官内T、B淋巴细胞较对照组成熟快、数量增多、产生抗体增多 ,免疫功能增强 ;③试验组前肠粘膜皱褶增多、隐窝加深、微绒毛长而密集 ,肠吸收面积增大 ,为鲤鱼增重、防病治病提供理论依据     

Experimental studies with foot-and-mouth disease virus type Asia-1, responsible for the 2005 epidemic in China

This study was carried out to investigate the biological characteristics of the foot-and-mouth disease (FMD) virus strain Asia-1 China/2005, which is responsible for the 2005 epidemic in China. The result showed that this strain is not host restricted, and could not only cause FMD in cattle and sheep but also in pigs by either inoculation or direct contact.     

新训犬员"四做到"

新训犬员由于初次带犬,还不能很好的掌握犬的行为特点和生活习性,个别人甚至对犬存在畏惧心理,在操作技能上也是一片空白,有时会觉得无从下手。如何提高新训犬员的基本技能,笔者认为应做到以下四个方面：