盘江黄牛及其杂交牛育肥中干物质、粗蛋白、综合净能需要研究

将３６ 头盘江黄牛及其杂交牛分成１２ 组，采用不同的日粮，进行两期育肥试验，得到不同的增重水平和实际营养物质摄入量。用各组实际采食的干物质（ ＤＭ） 、粗蛋白（ＣＰ） 、综合净能（ＮＥｍｆ） 与我国试行的《肉牛饲养标准》进行比较。结果表明，除干物质与标准相差较多（ － ６ ．３ ％ ） 外，粗蛋白和综合净能与标准相当接近（ 仅相差－ １ ．５ ％ 和２ ．８ ％ ） ，说明盘江黄牛及其杂交牛在育肥中的营养需要基本与标准相符，在配制日粮时，可以参考我国的《肉牛饲养标准》。     

Prokaryotic Expression and Bioinformatics Analysis of Nonstructure Protein 3C from Porcine Encephalomyocarditis Virus

To obtain the 3C protein of encephalomypcarditis virus (EMCV) and predict its structure,3C gene was amplified by RT-PCR with the RNA as template from HB10 strain. And the target gene inserted into pMD18-T vector. The plasmids were sequenced after verification by PCR and double enzyme digestion. The target gene was cleaved from the correct plasmid,and inserted into the pET32a vector. The recombinant pET32a-EMCV-3C was transformed into TranSetta (DE3) and then induced by IPTG. Then the size was identified by SDS-PAGE and the antigenicity was analyzed by Western blotting. According to the sequence results,the length of 3C gene was 615 bp,which encoded 205 amino acids. The results of SDS-PAGE showed that His-3C protein mainly existed in the form of inclusion body with the molecular of 40 ku. The Western blotting results verified that purified His-3C could react with rabbit anti-EMCV serum prepared with EMCV. Bioinformatics analysis indicated that 3C protein was non-secretory,and had multiple phosphorylation sites,but it had no transmembrane region. Thus,the 3C protein mainly involved in protein hydrolysis process.3C protease,as the only protease in the EMCV genome,played an indispensable role in viral replication. In this study,we successfully constructed the prokaryotic expression vector of 3C protein and predicted its structure,which provided a basis for further study of the role of 3C protein and catalytic mechanism.     

北方蚕业科研协作区第六批桑品种鉴定(陕西点)试验报告

2015—2016年对北方蚕业科研协作区4家单位的9个桑树新品种进行了共同鉴定。本批参鉴品种中,生长势旺、产叶量高、叶质好的品种有鲁插2号、鄂桑2号、陕桑9312、陕桑8826,其中鲁插2号叶片大而厚、发条力强,鄂桑2号节间密、片叶率高。     

密集烘烤后期不同风机转速对烤后烟叶内在品质的影响

为探索当前密集烘烤过程里循环风机不同转速对烤后烟叶内在品质的影响,分析烘烤后期循环风机转速与烟叶香吃味之间的关系,试验以烟草品种南江3号中部叶为材料,研究了干筋期烟叶烘烤循环风机转速对烤后烟叶主要化学成分协调性和评吸质量的影响.结果表明,烘烤开始至52℃范围内风机转速采用1 450 r/min、52～60℃范围内风机转速采用960 r/min、60～65℃至烘烤结束风机转速采用720r/min进行通风,可以提高烤后烟叶内在品质,促进化学成分协调性,有利于石油醚提取物含量的积累,能够明显改善烤后烟叶的香吃味.     

鹌鹑原始生殖细胞的分离及冷冻

以酶分解法消化孵化了5.5d的鹌鹑性腺得到鹌鹑PGCs,比较了3种不同酶消化时间所得的细胞数量;采用2种不同的冷冻方法冻存鹌鹑PGCs。结果表明：消化15min对于鹌鹑PGCs的分离最佳,而2种冷冻程序所得的活细胞数量差异不显著。     

Using the concept of ecological groundwater level to evaluate shallow groundwater resources in hyperarid desert regions

This paper,based on the analysis and calculation of the groundwater resources in an arid region from 1980 to 2001,put forward the concept of ecological groundwater level threshold for either salinity c...     

大豆萌发过程中谷氨酸脱羧酶活性变化研究

研究了大豆在浸泡、萌发两个过程中谷氨酸脱羧酶(GAD)的活力变化。通过测定大豆中γ-氨基丁酸(GABA)的含量变化,间接测定大豆中GAD的活性。结果表明,大豆在25℃,浸泡10h,大豆中的GAD活性达到浸泡中最高水平,酶活性为2.45U。浸泡后的大豆在30℃,萌发40h时,酶活性达到萌发中最高水平,为1.28U。大豆在浸泡和萌发两个状态下,GAD活性较储藏期都有很大提高,且浸泡时酶活总体水平高于萌发期酶活总体水平,其中大豆GAD在25℃,浸泡10h时活性最高,为2.45U。     

报废汽车回收拆解的绿色无害化管理

对我国报废汽车回收拆解过程中的生产者责任、回收拆解企业要求和零部件再制造进行了分析讨论,在此基础上提出了报废汽车回收拆解过程中的绿色无害化管理的改进意见和建议.     

Mcl-1 involves in G2/M arrest of HL-60 cells induced by diallyl disulfide

AIM: To investigate the role of Mcl-1 in the G₂/M arrest induced by diallyl disulfide (DADS) in leukemic HL-60 cells.METHODS: The inhibitory effect of DADS on human leukemic HL-60 cells was detected by CCK-8 method in vitro. Flow cytometry analysis was employed to observe the cycle arrest in HL-60 cells and the effect of DADS-induced G₂/M arrest on HL-60 cells with Mcl-1 gene knockdown by RNAi silencing. The expression of Mcl-1, PCNA and CDK1 in HL-60 cells treated with DADS was determined by Western blotting. The binding of Mcl-1 with PCNA and CDK1 was detected by coimmuno-precipitation. RESULTS: HL-60 cells were treated with DADS at concentration of 15, 30, 60, 120 or 240 μmol/L for 48 h. The inhibition rates of HL-60 cell proliferation were 31.15%, 55.88%, 66.14%, 75.29% and 80.35%, respectively, and gradually enhanced with the increase in the concentration of DADS (P<0.05). Flow cytometry analysis revealed that the proliferation of HL-60 cells was blocked by DADS in the G₂/M phase. Treatment with DADS for 24 h and 48 h at concentrations of 60 μmol/L and 120 μmol/L, the percentage of G₂/M phase cells increased as compared to the untreated cells (P<0.05). DADS induced arrest of HL-60 cells in G₂/M phase in a time- and dose- dependent manner (P<0.05). The results of Western blot analysis indicated that Mcl-1, PCNA and CDK1 in HL-60 cells were significantly reduced after treated with DADS (P<0.05). HL-60 cell cycle progression delayed by silencing Mcl-1 gene with siRNA technique, suggesting that silence of Mcl-1 gene led to G₂/M arrest. Compared to the cells treated with DADS only, the percentage of G₂/M cells raised in the cells with Mcl-1 gene silencing and treated with DADS (P<0.05), indicating that Mcl-1 gene silencing enhanced the effect of DADS-induced G₂/M arrest in HL-60 cells. The binding of Mcl-1 with PCNA and CDK1 was detected by coimmuno-precipitation and the formation of heterodimers was observed, which was decreased after treated with DADS for 4 h.CONCLUSION: DADS inhibits the proliferation of HL-60 cells and induces its G₂/M phase arrest. The decreased expression of PCNA is related to inhibiting the proliferation of leukemic cells. Knockdown of Mcl-1 gene enhances the effect of DADS-induced G₂/M arrest.