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81.
Lauritzen B Lykkesfeldt J Skaanild MT Angen Ø Nielsen JP Friis C 《Research in veterinary science》2003,74(3):261-270
Biomarkers of infection were screened for their possible role as evaluators of antibiotic treatment in an aerosol infection model of porcine pneumonia caused by Actinobacillus pleuropneumoniae (Ap). Following infection of 12 pigs, clinical signs of pneumonia developed within 20 h, whereafter the animals received a single dose of either danofloxacin (2.5mg/kg) or tiamulin (10 mg/kg). To test the discriminative properties of the biomarkers, the dosage regimens were designed with an expected difference in therapeutic efficacy in favour of danofloxacin. Accordingly, the danofloxacin-treated pigs recovered clinically within 24h after treatment, whereas tiamulin-treated animals remained clinically ill until the end of the study, 48 h after treatment. A similar picture was seen for the biomarkers of infection. During the infection period, plasma C-reactive protein (CRP), interleukin-6 and haptoglobin increased, whereas plasma zinc, ascorbic acid and alpha-tocopherol decreased. In the danofloxacin-treated animals, CRP, interleukin-6, zinc, ascorbic acid and alpha-tocopherol reverted significantly towards normalisation within 24h of treatment. In contrast, signs of normalisation were absent (CRP, zinc and ascorbic acid) or less marked (interleukin-6 and alpha-tocopherol) in the tiamulin-treated animals. Plasma haptoglobin remained elevated throughout the study in both groups. This indicates that CRP, zinc, ascorbic acid and to a lesser extent interleukin-6 and alpha-tocopherol might be used to evaluate antibiotic treatment of acute Ap-infection in pigs. The present model provides a valuable tool in the evaluation of antibiotic treatments, offering the advantage of clinical and pathological examinations combined with the use of biochemical infection markers. 相似文献
82.
Resistance of porcine mycoplasmas to heat 总被引:1,自引:0,他引:1
N F Friis 《Acta veterinaria Scandinavica》1974,15(2):283-285
83.
N F Friis 《Acta veterinaria Scandinavica》1972,13(2):284-286
In a study on the distribution of Mycoplasma sui- (hyo-) pneumoniae (M. suip.) among Danish swine it was found that most isolates possessing the characteristic colonial morphology of M. suip. would be inhibited significantly in the growth inhibition (g.i.) and metabolic inhibition (m.i.) tests by antiserum for a type strain* of this species. However, a few isolates were found to be completely unaffected by this antiserum. Five such strains have been recovered, viz. 4 from cases of catarrhal pneumonia in bacon pigs, 1 from the nasal cavity of a 40-kg pig. The pigs in question originated from 5 different herds. The recovery of 1 of the strains has been reported (Friis 1971b). 相似文献
84.
85.
86.
Anita Wengel Niels Jacobsen Hans Kolind-Andersen Poul Bjerregaard 《Pest management science》1990,30(2):223-233
Several thiosemicarbazones of 2-formyl- and 2-acetylpyridine dialkylated on N4 and non-alkylated on N2 were found to be broad-spectrum protectant fungicides with activity particularly against oomycetes. The effect of some of the compounds on chitin biosynthesis was studied, but the low inhibitory activity observed combined with the fungicidal activity spectrum—particularly the high activity against oomycetes—excludes this as their main mode of action. Attempts at enhancing the systemic properties of the compounds by chemical modifications failed. 相似文献
87.
88.
The aim of the study was to examine the ability of Haemophilus somnus and Mycoplasma dispar to induce pneumonia in healthy calves under conditions closely resembling the supposed natural way of infection, viz. by inhalation of aerosol droplets containing the microorganisms. The infections were investigated by recording clinical data, cytokine expression of peripheral blood cells and pathology. Twelve calves were included in the study: Three animals were exposed to H. somnus only, and two to M. dispar only, whereas five were challenged to M. dispar followed by exposure to H. somnus 11-14 days later. Also, one calf was exposed to M. dispar followed by exposure to a sterile saline solution 11 days later, and one calf was only exposed to a sterile saline solution. Just one animal, only challenged with H. somnus, developed a focal necrotizing pneumonia, from which H. somnus was isolated. Thus, the ability of H. somnus and M. dispar to act as primary pathogens under these conditions were minimal and inconsistent.However, a transient rise in body temperature, a marked granulocytosis and increased levels of interleukin-8 in peripheral blood after inoculation with H. somnus indicated a clear systemic response, probably as a consequence of the natural non-specific local and systemic defence mechanisms acting in healthy calves. 相似文献
89.
The pharmacokinetics and penetration of danofloxacin into the gastrointestinal tract in healthy pigs and in pigs experimentally infected with Salmonella typhimurium were studied.In the infected pigs, a decrease in body clearance and an increase in mean elimination half-life was observed (P < 0.01). Moreover a significant reduction in the volume of the peripheral compartment was found. Danofloxacin distributed well to the gastrointestinal tract achieving high AUC / AUC(plasma)ratios in both groups of pigs. However, compared to the healthy pigs AUC / AUC(plasma)ratios decreased in the infected pigs. Salmonella infection led to an increase in mean residence time (MRT) in the small intestines and lymph nodes and a decrease in MRT in caecum and colon. 相似文献
90.
Narong TIPTANAVATTANA Araya RADTANAKATIKANON Poul HYTTEL Hanne HOLM Supranee BURANAPRADITKUN Piyathip SETTHAWONG Mongkol TECHAKUMPHU Theerawat THARASANIT 《The Journal of reproduction and development》2015,61(6):581-588
The development of germ cells has not been entirely documented in the cat especially the transition phase of
the gonocyte to the spermatogonial stem cell (G/SSC). The aims of study were to examine testicular development
and to identify the G/SSC transition in order to isolate and culture SSCs in vitro. Testes
were divided into 3 groups according to donor age (I, < 4 months; II, 4–6 months; and III, > 6 months).
In Exp. 1, we studied testicular development by histology, transmission electron microscopy and
immunohistochemistry. In Exp. 2, we determined the expression of GFRα-1, DDX-4 and c-kit and performed flow
cytometry. The SSCs isolated from groups II and III were characterized by RT-PCR and TEM (Exp. 3).
Chronological changes in the G/SSC transition were demonstrated. The size, morphology and ultrastructure of
SSCs were distinguishable from those of gonocytes. The results demonstrated that group II contained the
highest numbers of SSCs per seminiferous cord/tubule (17.66 ± 2.20%) and GFRα-1+ cells (14.89 ±
5.66%) compared with the other groups. The findings coincided with an increased efficiency of SSC derivation
in group II compared with group III (74.33 ± 2.64% vs. 23.33 ± 2.23%). The colonies expressed
mRNA for GFRA1, ZBTB16, RET and POU5F1.
Our study found that the G/SSC transition occurs at 4–6 months of age. This period is useful for isolation and
improves the establishment efficiency of cat SSCs in vitro. 相似文献